Search Results : NOVA-4244-03 Num PRODUCTS MATCHED : 1

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NEXTflex™ Congenital Adrenal Hyperplasia Amplicon Panel for Illumina platforms (Barcodes 1- 96) Bioo Scientific NOVA-4244-03 96 rxns £2781.00
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Product Detail: The NEXTflex™ Congenital Adrenal Hyperplasia Amplicon Panel allows you to quickly target and sequence 10 exons of the CYP21A2 locus. This kit contains primer pairs and reagents needed to amplify these coding regions of the CYP21A2 gene from DNA isolated from fresh or frozen material, and prepare libraries for Illumina® sequencing.

Traditional amplicon sequencing of CYP21A2 can be confounded by pseudogene CYP21A1P, which has 99% homology with CYP21A2. In order to obtain ‘true’ CYP21A2 reads, the NEXTflex Congenital Adrenal Hyperplasia Amplicon Panel uses a two-step enrichment. In the first step, sample DNA is enriched for CYP21A2 DNA by long-range PCR. In the second step, ten canonical exons are targeted and amplified. During a gene conversion event, CYP21A2 and CYP21A1P can transfer pseudogene sequences into the CYP21A2 gene. To detect CYP21A2 mutations introduced by the pseudogene, enrichment primers are designed to amplify both CYP21A2 and CYP21A1P with the same efficiency.

While up to 384 barcodes are currently supplied with the NEXTflex Congenital Adrenal Hyperplasia Amplicon Panel, the panel has the capability of multiplexing up to 950 samples at 100x coverage for detection of germline mutations on a single Illumina® 2x300 MiSeq® lane. If you need the ability to multiplex more than 384 samples, contact nextgen@biooscientific.com to request a quote for a panel with additional barcodes.


A Complete Solution for Targeted CYP21A2 Sequencing

The NEXTflex Congenital Adrenal Hyperplasia Amplicon Panel is a complete, validated solution that targets 10 exons in the CYP21A2 locus using 20 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex Congenital Adrenal Hyperplasia Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.



[BENEFITS]
•100% coverage of the CYP21A2 exons and flanking intron-exon boundaries
•100% uniformity of amplicons on 0.2x coverage
•384 unique barcodes available
•Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
•Low input - Only 20 ng DNA isolated from fresh or frozen samples required

Product Type: NGS - Illumina Amplicon Panels
Applications: Congenital Adrenal Hyperplasia Amplicon Panel
Search Terms: Illumina Congenital Adrenal Hyperplasia Amplicon Panel
Application Details: Please use our contact form above to request a copy of the datasheet
Catalogue Number Update: Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4244-03
NGS Applications: Congenital Adrenal Hyperplasia Amplicon Panel