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Product - please click for more details Brand Cat No Size Price (£)
NEXTflex™ Diabetes-3 Amplicon Panel for Illumina platforms (8 Barcodes) Bioo Scientific NOVA-4243-01 8 rxns £489.00
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Product Detail: The NEXTflex™ Diabetes-3 Amplicon Panel allows you to quickly target and sequence 17 exons of the KLF11, NEUROD1, CEL, and PDX1 loci. This kit contains primer pairs and reagents needed to amplify these coding regions of the KLF11, NEUROD1, CEL, and PDX1 genes from DNA isolated from fresh or frozen material, and prepare libraries for Illumina® sequencing.

While up to 384 barcodes are currently supplied with the NEXTflex Diabetes-3 Amplicon Panel, the panel has the capability of multiplexing up to 750 samples at 100x coverage for detection of germline mutations on a single Illumina® 2x300 MiSeq® lane. If you need the ability to multiplex more than 384 samples, contact nextgen@biooscientific.com to request a quote for a panel with additional barcodes.


A Complete Solution for Targeted KLF11, NEUROD1, CEL and PDX1 Sequencing

The NEXTflex Diabetes-3 Amplicon Panel is a complete, validated solution that targets 17 exons in the KLF11, NEUROD1, CEL, and PDX1 loci using 44 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex Diabetes-3 Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.



[BENEFITS]
•100% coverage of 17 exons of the KLF11, NEUROD1, CEL and PDX1 exons and flanking intron-exon boundaries
•100% uniformity of amplicons on 0.2x coverage
•384 unique barcodes available
•Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
•Low input - Only 20 ng DNA isolated from fresh or frozen samples required

Product Type: NGS - Illumina Amplicon Panels
Applications: Diabetes-3 Amplicon Panel
Search Terms: Illumina Diabetes-3 Amplicon Panel
Application Details: Please use our contact form above to request a copy of the datasheet
Catalogue Number Update: Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4243-01
NGS Applications: Diabetes-3 Amplicon Panel