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Product - please click for more details Brand Cat No Size Price (£)
NEXTflex™ Mediterranean Fever Amplicon Panel Illumina-Compatible (8 Barcodes) Bioo Scientific NOVA-4237-01 8 rxns £277.00
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Product Detail: The NEXTflex™ Mediterranean Fever Amplicon Panel allows you to quickly target and sequence 11 exons of the MEFV loci. This kit contains primer pairs and reagents needed to amplify these coding regions of MEFV from DNA isolated from fresh or frozen samples and prepare libraries for Illumina sequencing.

While up to 384 barcodes are currently supplied with the NEXTflex Mediterranean Fever Amplicon Panel, the panel has the capability of multiplexing up to 1600 samples at 100x coverage for detection of germline mutations on a single Illumina 2x300 MiSeq lane.

A Complete Solution for Targeted MEFV Sequencing:

The NEXTflex Mediterranean Fever Amplicon Panel is a complete, validated solution that targets 11 exons in MEFV loci using 24 PCR primer pairs. This kit requires only 20 ng of genomic DNA. This kit can be easily automated on liquid handlers.

Kit Specs

The NEXTflex Mediterranean Fever Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® compatible sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

•100% coverage of MEFV and flanking intron-exon boundaries (4 kb total)
•100% uniformity of amplicons at 0.2X mean coverage
•Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads and up to 384 barcodes
•Low input - Only 20 ng DNA required
•Manufactured to the international quality standard ISO 13485

This kit is manufactured to the international quality standard ISO 13485.

Product Type: NGS - Illumina Amplicon Panels
Applications: Illumina Mediterranean Fever Amplicon Panel
Search Terms: Mediterranean Fever Amplicon Panel
Application Details: Please use our contact form above to request a copy of the datasheet
Catalogue Number Update: Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4237-01
NGS Applications: Mediterranean Fever Panel