Search Results : NOVA-4210-07 Num PRODUCTS MATCHED : 1

Product - please click for more details Brand Cat No Size Price (£)
NEXTflex 18S ITS Amplicon-Seq Kit (Barcodes 289 -384) Bioo Scientific NOVA-4210-07 192 rxns £2071.00
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Product Detail: The NEXTflex 18S ITS Amplicon-Seq Kit is designed to prepare multiplexed amplicon libraries that span the hypervariable Internal Transcribed Spacer (ITS) region of eukaryotic 18S ribosomal RNA (rRNA) genes for identification of uncultured fungal and micro-eukaryotic organisms. These libraries are compatible with paired-end sequencing on the Illumina® MiSeq platform.

Together, the ease of performing library preparation, extreme multiplexing capabilities obtained with the NEXTflex 18S ITS Amplicon-Seq Kit and the high sensitivity of NGS provide a convenient and powerful method to detect unique components of eukaryotic communities.
Background Info: All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type: NGS - 18s
Applications: 18S ITS, Metagenomics
Search Terms: 18S ITS, Metagenomics
Additional Info: [[ Fast Library Prep Protocol ]]

There are two steps involved in 18S ITS amplicon processing: an initial PCR amplification using customized PCR primers that target the ITS domains, and a subsequent PCR amplification that integrates relevant flow cell binding domains and unique 12 base pair sample indices. A limited number of cleanup steps ensures maximum recovery of amplicons for downstream sequencing. It is highly recommended that sequencing of 18S ITS libraries be performed using the MiSeq V3 reagent kit (2X300).


[[ Optimized 18S Amplicon-Seq Protocol Offers Lower PCR Bias and Fewer Off-target Reads ]]

The protocol incorporated in the NEXTflex 18S ITS Amplicon-Seq Kit offers better sequencing results than can be obtained using traditional 18S sequencing protocols. The incorporation of the second PCR step in the protocol for the addition of the sample-specific index reduces the number of off-target reads typically encountered during amplicon sequencing.


[[ Sequencing of the 18 S rRNA Gene ]]

The internal transcribed spacer region (ITS) within the highly conserved 18Ss rRNA gene is the gold standard used in eukaryotic molecular phylogeny. The ITS region contains a high level of sequence variation between closely related species and is an easy target to amplify from minimal quantities of DNA due to the high number of copies found per genome, ranging from 10-200. This is an excellent marker for detecting both rare species and those in great abundance within complex communities.

[BENEFITS]
>>> Low PCR bias and high on-target reads
>>> Fast library prep kit protocol
>>> Low input – As low as 1 ng of genomic DNA
>>> Flexible barcode options– Up to 384 unique barcodes available for multiplexing of libraries
>>> Compatible with regular Illumina sequencing primers
>>> Functionally validated with Illumina HiSeq 2000 and MiSeq sequencing platforms
>>> Automation-friendly workflow is compatible with liquid handlers

For larger volume requirements, customized and bulk packaging is available. Please email us to tech@nktscientific.com for further information.
Application Details: Please use our contact form above to request a copy of the datasheet
Catalogue Number Update: Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4210-07