| Product - please click for more details |
Brand |
Cat No |
Size |
Price (£) |
|
| MaxSuppressor In Vivo RNA-LANCEr II |
Bioo Scientific |
3410-01 |
20 injections |
£550.00
|
|
| Product Detail: |
MaxSuppressor In Vivo RNA-LANCEr II is a patent-pending, proprietary formulation composed of neutral lipid, non-ionic detergent, oil and small molecules which enable highly efficient delivery of RNAi agents into animals. This novel product has been shown to be more robust and efficient at penetrating tissues and organs than other delivery agents.
The procedure to package RNAi agents into the phospholipid-oil emulsion takes less than 1-hour. To use MaxSuppressor In Vivo RNA-LANCEr II simply mix 100 ug of your RNAi agent with a single tube of semi-dry MaxSuppressor In Vivo RNA-LANCEr II agent in total volume of less than 300 uL, in 1X PBS; extrude using the Lipid Extruder (Bioo Scientific cat. #: 341007) with the supplied 100 nm pore sizing filters (or sonicate) and then directly inject into the animal.
BENEFITS:-
Robust in vivo delivery agent - efficient at penetrating tissues and organs
Flexible works with multiple RNAi agents and routes of delivery
Gentle and non-toxic
Custom solutions for delivery available
|
| Background Info: |
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans. |
| Product Type: |
Small RNA Cloning & Delivery |
| Additional Info: |
TESTS PER KIT: The kit has the capacity for 20 injections.
SHELF LIFE: The shelf life of the kit is 12 months at -20ºC before RNAi agent is added to the vial and 3 months at -80ºC once the RNAi agent has been added to the vial. |
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| Lipid Extruder |
Bioo Scientific |
341007 |
each |
£680.00
|
|
| Product Detail: |
The Lipid Extruder is intended for use with MaxSuppressor RNA-LANCEr II (Bioo Scientific cat. # 3410-01) for the purpose of homogenizing lipid particles to 100 nm prior to the in vivo administration of the RNAi agent. Upon testing with MaxSuppressor In vivo RNA LANCEr II, we have found that extrusion to 100 nm particle size generates a homogeneous size range of particles and increases the delivery efficiency of the agent.
Lipid extrusion is a technique in which a lipid suspension is forced through a polycarbonate filter with a defined pore size to yield particles having a diameter near the pore size of the filter used. Extrusion through filters with 100 nm pores typically yields large, unilamellar vesicles with a mean diameter of 120-140 nm. Mean particle size also depends on the lipid composition and is quite reproducible from batch to batch. The extrusion should be done at a temperature above the Tc of the lipid. Attempts to extrude below the Tc will be unsuccessful as the membrane has a tendency to clog with rigid lipid particles that cannot pass through the pores. The Lipid Extruder allows researchers to prepare unilamellar vesicles by extrusion in an efficient, rapid manner. The transition temperature (Tm) of MaxSuppressor RNA LANCEr II is below room temperature and thus, no heating or temperature regulation of the device is required. The filter housing is constructed of stainless steel and Teflon allowing for rapid cleaning and autoclaving of all parts.
BENEFITS:-
Increases RNAi agent delivery efficiency
Produces homogeneously sized particles
Relatively inexpensive
|
| Background Info: |
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans. |
| Product Type: |
Small RNA Cloning & Delivery |
|
