Product Search Results : Fructose Assay


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Bioo Scientific 1309-01 48 rxns Call for Pricing
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Product Detail: The MaxSignal Fructose Assay Kit is a cuvette-based enzymatic assay for the determination of fructose across a variety of food samples. The assay is useful for analysis of inverted sugar, a mixture of equal parts of glucose and fructose resulting from the hydrolysis of sucrose; this sugar is found naturally in fruits and honey and produced artificially for use in the food industry. The assay can also be performed using a microtiter plate for high-throughput sample analysis.

The MaxSignal Fructose Assay Kit uses a highly-specific coupled enzymatic reaction to detect fructose in food samples: Fructose is first specifically converted to fructose-6-phosphate (F-6-P) using hexokinase. Next the F-6-P is converted to glucose-6-phosphate (G-6-P) using the enzyme phosphoglucose isomerase (PGI). The G-6-P compound is then used as a substrate by the enzyme glucose-6-phosphate dehydrogenase to convert NADP+ to NADPH. This conversion, measured at 340 nm, is proportional to the level of fructose in the sample.

The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of fructose in each sample is then directly determined from the change in absorbance at 340 nm within 30 minutes.

This kit is manufactured to the international quality standard ISO 9001:2008.
ISO CI#: SARA-2009-CA-0114-01-B
• High sensitivity and low detection limit: 0.3 mg/L (ppm) fructose
• A rapid (30 minutes), robust enzyme-based assay
• High reproducibility
• Flexible assay format

Background Info: All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type: Food safety - Constituents
Additional Info: The MaxSignal Fructose Assay Kit has the capacity for 48 determinations or 24 determinations in duplicate. Store the kit at 4°C. The shelf life of the kit is 12 months, after receipt, when the kit is properly stored.
Application Details: Please use our contact form above to request a copy of the datasheet and pricing