CpxA (Conjugative Plasmid Expression) is the 458 amino acid protein, a bacterial inner membrane histidine protein kinase, responsible for sensing misfolded proteins in the periplasm. It is part of CpxA/CpxR two component signal transduction system, where CpxR is the cognate response regulator. In sensing bacterial envelope stress, the Cpx pathway regulates the production of a number of periplasmic folding and trafficking factors to maintain outer membrane integrity. It is also responsible for global control of virulence factor expression. Alternative name: Integral membrane sensor signal transduction histidine kinase.
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Yersinia pestis, Yersinia enterocolica and related species
Recombinant CpxA (residues 26 to 168) over-expressed as a N-terminal fusion with His(x6)-tag, which was removed prior to immunization. The antigen originates from enteropathogenic Yersinia pseudotuberculosis YPIII; B1JQV1, NCBI annotated locus tag YPK_4133.
Plant protein extraction buffer Secondary antibodies
Expected | apparent MW:
51.8 | 52-53 kDa
Not reactive in:
no confirmed exceptions from predicted reactivity are currently known
to be added when available, antibody released in April 2013
Special application note:
The strains were grown in LB broth until late stationary phase (absorbance at 600nm of ~1.5). To avoid interference from cross-reacting bands, aim to maximize protein separation with the region of 35 to 55 kDa. CpxA could be routinely detected from a 20 µL of sample that was derived from 1ml of pelleted bacteria resuspended in 200 µl of 1x loading buffer. Re-use of diluted working strength antibody is not recommended.
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