Dynactin subunit 1 (DCTN1) is the largest (approximately 150 kD) subunit of dynactin, a macromolecular complex consisting of multiple subunits ranging in from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse range of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis.
DCTN1 contains N-terminal CAP-Gly and basic domains, followed by the coiled-coil (CC) 1 and 2 domains [1]. The CAP-Gly and basic domains form the microtubule binding domain (MTBD) [2] and the CC1 and CC2 domains mediate the interactions with dynein intermediate chain (DIC) and the other dynactin subunits [1]. The MTBDs of DCTN1 allow microtubule binding during spindle formation and chromosome movement making them essential for cell division[3],
Mutations in the DCTN1 gene are associated with amyotrophic lateral sclerosis (ALS) and distal hereditary motor neuronopathy type VIIB (HMN7B/spinal and bulbar muscular atrophy/dSBMA).
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 100 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Guinea Pig, Sheep, Goat, Golden Hamster, Cat, Pig
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
Immunogen:
Partial length recombinant human DCTN1 from the C-terminal region
If you would like further information regarding the immunogen used in the productiion of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
142kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Subcellular location:
Cytoplasm cytoskeleton
Purification:
Affinity purification
References:
1: Schroer TA. Dynactin. Annu Rev Cell Dev Biol. 2004;20:75979. 2: Culver-Hanlon TL, Lex SA, Stephens AD, Quintyne NJ, King SJ. A microtubule-binding domain in dynactin increases dynein processivity by skating along microtubules. Nat Cell Biol. 2006;8(3):26470. 3: Robinson RW, Snyder JA. Colocalization studies of Arp1 and p150Glued to spindle microtubules during mitosis: the effect of cytochalasin on the organization of microtubules and motor proteins in PtK1 cells. Cell Biol Int. 2006;30(7):6319.
Dynactin subunit 1 (DCTN1) is the largest (approximately 150 kD) subunit of dynactin, a macromolecular complex consisting of multiple subunits ranging in from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse range of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis.
DCTN1 contains N-terminal CAP-Gly and basic domains, followed by the coiled-coil (CC) 1 and 2 domains [1]. The CAP-Gly and basic domains form the microtubule binding domain (MTBD) [2] and the CC1 and CC2 domains mediate the interactions with dynein intermediate chain (DIC) and the other dynactin subunits [1]. The MTBDs of DCTN1 allow microtubule binding during spindle formation and chromosome movement making them essential for cell division[3],
Mutations in the DCTN1 gene are associated with amyotrophic lateral sclerosis (ALS) and distal hereditary motor neuronopathy type VIIB (HMN7B/spinal and bulbar muscular atrophy/dSBMA).
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
50 µg in 50 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Guinea Pig, Sheep, Goat, Golden Hamster, Cat, Pig
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
Immunogen:
Partial length recombinant human DCTN1 from the C-terminal region
If you would like further information regarding the immunogen used in the productiion of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
142kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Subcellular location:
Cytoplasm cytoskeleton
Purification:
Affinity purification
References:
1: Schroer TA. Dynactin. Annu Rev Cell Dev Biol. 2004;20:75979. 2: Culver-Hanlon TL, Lex SA, Stephens AD, Quintyne NJ, King SJ. A microtubule-binding domain in dynactin increases dynein processivity by skating along microtubules. Nat Cell Biol. 2006;8(3):26470. 3: Robinson RW, Snyder JA. Colocalization studies of Arp1 and p150Glued to spindle microtubules during mitosis: the effect of cytochalasin on the organization of microtubules and motor proteins in PtK1 cells. Cell Biol Int. 2006;30(7):6319.
Uniprot ID:
Q14203
Gene ID:
1639
Gene symbol:
DCTN1
Release Date:
May-19
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