| Product Detail: |
Anti-CPT1A Antibody (Polyclonal) |
| Background Info: |
Carnitine palmitoyltransferase I (CPT1) is a liver enzyme involved in fatty acid oxidation. Three isoforms of CPT1 are currently known: CPT1A, CPT1B, and CPT1C. This antibody recognises CPT1A.
The carnitine palmitoyltransferase (CPT) enzyme system, with acyl-CoA synthetase and carnitine/acylcarnitine translocas, provide the mechanism for long-chain fatty acids to be transferred from the cytosol to the mitochondrial matrix to undergo beta-oxidation for energy production.
The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase.
Carnitine palmitoyltransferase I is responsible for the formation of acyl carnitines, catalysing the transfer of the acyl group of a long-chain fatty acyl-CoA from coenzyme A to l-carnitine, allowing movement of the acyl carnitine from the cytosol into the intermembrane space of mitochondria. CPT I is key for carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. |
| Product Type: |
NS Reagents Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
100 µg in 100 µl PBS containing 0.02% sodium azide. |
| Storage Temp: |
6 months: 4°C. Long-term storage: -20°C. Avoid multiple freeze and thaw cycles. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Human, Mouse |
| Expected Species: |
Rat, Bovine, Goat
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you. |
| Immunogen: |
Partial length recombinant human CPT1A from the C-terminal region |
| Applications: |
WB ¦ IF ¦ IP |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you. |
| Antibody Isotype: |
IgG |
| Application Details: |
WB 1:500-2000. IF 1:50-200. IP 1:50-100. |
| Category: |
Primary Antibodies |
| Other names: |
CPT1, CPT1-L, L-CPT1, Carnitine palmitoyltransferase IA |
| Research Areas: |
Cell Signalling and Transport, Transcription and Translation, Protein Modification, Transcription, Chromatin |
| NS Reagents Product Area: |
Cell Signalling |
| Peptide Sequence (Internal Use only): |
GYAEDGHCKGDINPNIPYPTRLQWDIPGECQEVIETSLNTANLLANDVDFHSFPFVAFGKGIIKKCRTSPDAFVQLALQLAHYKDMGKFCLTYEASMTRLFREGRTETVRSCTTESCDFVRAMVDPAQTVEQRLKLFKLASEKHQHMYRLAMTGSGIDRHLFCLYVVSKYLAVESPFLKEVLSEPWRLSTSQTPQQQVELFDLENNPEYVSSGGGFGPVADDGYGVSYILVGENLINFHISSKFSCPETGIISQGPSSDT
Recombinant fusion protein containing a sequence corresponding to amino acids 497-756 of human CPT1A.
Full length protein is 770 aa long (Variant 1) but variant 2 (P50416-2) is the full sequence above. Antibody should bind both variants due to sequence homology
10 20 30 40 50
MAEAHQAVAF QFTVTPDGID LRLSHEALRQ IYLSGLHSWK KKFIRFKNGI
60 70 80 90 100
ITGVYPASPS SWLIVVVGVM TTMYAKIDPS LGIIAKINRT LETANCMSSQ
110 120 130 140 150
TKNVVSGVLF GTGLWVALIV TMRYSLKVLL SYHGWMFTEH GKMSRATKIW
160 170 180 190 200
MGMVKIFSGR KPMLYSFQTS LPRLPVPAVK DTVNRYLQSV RPLMKEEDFK
210 220 230 240 250
RMTALAQDFA VGLGPRLQWY LKLKSWWATN YVSDWWEEYI YLRGRGPLMV
260 270 280 290 300
NSNYYAMDLL YILPTHIQAA RAGNAIHAIL LYRRKLDREE IKPIRLLGST
310 320 330 340 350
IPLCSAQWER MFNTSRIPGE ETDTIQHMRD SKHIVVYHRG RYFKVWLYHD
360 370 380 390 400
GRLLKPREME QQMQRILDNT SEPQPGEARL AALTAGDRVP WARCRQAYFG
410 420 430 440 450
RGKNKQSLDA VEKAAFFVTL DETEEGYRSE DPDTSMDSYA KSLLHGRCYD
460 470 480 490 500
RWFDKSFTFV VFKNGKMGLN AEHSWADAPI VAHLWEYVMS IDSLQLGYAE
510 520 530 540 550
DGHCKGDINP NIPYPTRLQW DIPGECQEVI ETSLNTANLL ANDVDFHSFP
560 570 580 590 600
FVAFGKGIIK KCRTSPDAFV QLALQLAHYK DMGKFCLTYE ASMTRLFREG
610 620 630 640 650
RTETVRSCTT ESCDFVRAMV DPAQTVEQRL KLFKLASEKH QHMYRLAMTG
660 670 680 690 700
SGIDRHLFCL YVVSKYLAVE SPFLKEVLSE PWRLSTSQTP QQQVELFDLE
710 720 730 740 750
NNPEYVSSGG GFGPVADDGY GVSYILVGEN LINFHISSKF SCPETDSHRF
760 770
GRHLKEAMTD IITLFGLSSN SKK |
| Molecular Weight: |
88kDa (Intended as a general guide and does not allow for all isoforms and species variations) |
| Subcellular location: |
Mitochondrion outer membrane, Multi-pass membrane protein |
| Purification: |
Affinity purification |
| Uniprot ID: |
P50416 |
| Gene ID: |
1374 |
| Gene symbol: |
CPT1A |
| Release Date: |
November 2018 |
