Product Search Results : 5619-01


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Bioo Scientific 5619-01 1 x 96 wells £323.00
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Product Detail: • High sensitivity and wide linear range (0 - 4.5 mM)
• A rapid (5 minute) and robust enzyme-based assay which does not require expensive instrumentation
• High reproducibility.
The MaxDiscovery ?-Hydroxybutyrate Assay Kit is a plate-based colorimetric enzymatic assay for the quantitative determination of ?-Hydroxybutyrate in serum samples from rodents or other mammals.. The kit uses a spectrophotometric assay to detect ?-Hydroxybutyrate directly from serum samples, enabling researchers to detect ?-Hydroxybutyrate levels in animal serum and other tissue matrices such as urine and ascites.

The MaxDiscovery ?-Hydroxybutyrate Assay Kit is a simple, accurate and automation-compatible method for measuring ?-Hydroxybutyrate (BHBA) levels in serum samples. The kit uses a coupled enzyme reaction to detect BHBA as follows. First the BHBA is converted (oxidized) to acetoacetate by the enzyme BHBA dehydrogenase (BHBADH); in the process NAD+ is reduced to NADH. In the second step, the NADH product is used by the enzyme diaphorase to reduced a colorless tetrazolium salt, INT, to a brightly colored (red) formazan product, which absorbs light at 505 nm. Therefore the observed color change at 505 nm is directly proportional to the ?-Hydroxybutyrate concentration in the serum sample.

The kit contains sufficient materials to rapidly test 42 serum samples in duplicate. It also comes with a control solution containing a ?-Hydroxybutyrate standard (1 mM) which can be used to calibrate the assay.

?-Hydroxybutyrate, also named 3-hydroxybutyrate, is a ketone body primarily synthesized in the liver from the oxidation of fatty acids. Blood ?-Hydroxybutyrate (blood ketone) values provide information about impending or present ketoacidosis and ketotic states due to diabetes or other conditions. The measurement of ?-Hydroxybutyrate is essential for determining the severity of diabetic coma in diabetics. The insulin requirements are also based on the blood levels of ?-Hydroxybutyrate.
Background Info: All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type: Preclinical Chemistry - Blood Chemistry
Additional Info: The MaxDiscovery Beta-Hydroxybutyrate Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards). Store the kit at 4°C. The shelf life is 6 months after receipt when the kit is properly stored.