High sensitivity kit with a low detection limit (0.5 g/dL) A rapid 10 minute, robust enzyme-based assay which does not require expensive instrumentation High reproducibility
The MaxDiscovery Total Protein Assay Kit is a simple, direct and automation-compatible method for measuring total protein levels in serum samples. After preparing the sera, the assay is performed by adding Total Protein Reagent into microplate wells containing 6 µL sera. After a brief incubation, the absorbance of each well at 540 nm is then measured using a plate reader.
This method, commonly referred to as the Biuret assay, is based on the interaction of cupric ion (Cu2+) with protein in an alkaline solution.
Protein + Cu2+ ? blue-violet complex
The intensity of the color, and hence the absorption at 540 nm, is directly proportional to the protein concentration. This kit provides direct determination of total protein in serum, plasma, and other fluid samples.
Proteins form the major portion of the macromolecular solutes in the blood. Protein in the plasma is primarily comprised of albumin and globulin. Changes in blood protein levels are associated with various disease states. Elevated levels of total protein in plasma have been strongly associated with a variety of diseases involving the liver, kidney, or bone marrow, as well as other metabolic or nutritional disorders. Low serum protein concentrations can be symptoms of overhydration, kidney damage, severe burns, or failure of protein synthesis (starvation, protein malnutrition, or liver cell damage).
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.