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MaxDiscovery Aspartate Transaminase (AST) Color Endpoint Assay Kit Bioo Scientific 5605-01 1 x 96 wells £300.00
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Product Detail: The MaxDiscovery Aspartate Transaminase (AST) Color Endpoint Assay Kit is a plate-based colorimetric enzymatic assay for the determination of the aspartate transaminase enzyme in serum samples. Aspartate transaminase (AST) also known as aspartate aminotransferase or (sGOT) is a metabolic enzyme expressed primarily in the liver. Elevation of AST levels is an indication of liver damage and has been associated with liver injury. AST levels are monitored routinely in patients with liver diseases. AST is also a very useful tool for preclinical investigation of experimental drug formulations and AST levels are commonly used to monitor and attenuate the hepatotoxic effects of experimental drugs in rodents.

The MaxDiscovery Alanine Transaminase (AST) Color Endpoint Assay Kit uses a colored reaction scheme to detect AST enzymatic activity in serum samples. In this method aspartate and ?-ketoglutarate are first converted to glutamate and oxaloacetate. The oxaloacetate then reacts with diazonium salt to form a colored product. The concentration of AST in each sample is then directly determined from the absorbance at 510 nm measured with a plate reader. Dilutions of the oxaloacetate control, included in the kit, can be used to construct a standard curve to calibrate the assay and confirm assay linearity.

BENEFITS:
• High sensitivity and low detection limit (10 U/L)
• A rapid (20 min), robust enzyme-based assay
• Does not require expensive instrumentation
• High reproducibility
• Only requires 5 uL of serum
Background Info: All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type: Preclinical Chemistry - Toxicity
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
Additional Info: The MaxDiscovery Aspartate Transaminase (AST) Color Endpoint Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards). The kit also contains enough material to construct four standard curves. The shelf life of the kit is 6 months, after receipt, when the kit is properly stored.
Cat_no: 560501