| Product Detail: |
Rabbit Anti-Rat Aquaporin 4 Polyclonal |
| Background Info: |
Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 4 is constitutively expressed in the basolateral cell membrane of principal collecting duct cells in the kidney and provide a pathway for water to exit these cells (3). It is also expressed in astrocytes and is up regulated by direct insult to the CNS (4). Aquaporin 4 has been proposed as the primary autoimmune target in neuromyelitis optica ( 5, 6). |
| Product Type: |
Antibodies |
| Format: |
PBS, 50% glycerol, 0.09% sodium azide |
| Storage Temp: |
-20ºC |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Hu | Ms | Rt |
| Immunogen: |
Produced against the C-terminal peptide (Sequence N-CTKGSYMEVEDNRSQVETED) of rat aquaporin 4 |
| Applications: |
WB | IHC | ICC/IF |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
WB (1:2000), IHC (1:200), ICC/IF (1:400); optimal dilutions for assays should be determined by the user. |
| Target: |
Aquaporin 4 |
| Conjugate: |
HRP |
| Research Area(s): |
Neuroscience | Neurotransmission | Ion Channels | Aquaporins |
| Alternative Name(s): |
AQP 4 Antibody, AQP-4 Antibody, AQP4 Antibody, AQP4_HUMAN Antibody, Aquaporin type 4 Antibody, Aquaporin-4 Antibody, Aquaporin4 Antibody, HMIWC 2 Antibody, HMIWC2 Antibody, Mercurial insensitive water channel Antibody, Mercurial-insensitive water channel Antibody, MGC22454 Antibody, MIWC Antibody, WCH 4 Antibody, WCH4 Antibody |
| Category: |
Polyclonal |
| Immunogen Species: |
Rat |
| Accession Number: |
NP_036957.1 |
| Gene ID: |
25293 |
| Swiss-Prot: |
P47863 |
| Species Reactivity Full Name: |
Human | Mouse | Rat |
| Purification: |
Protein A Purified |
| Concentration: |
1 mg/ml |
| Specificity: |
Detects ~35kDa. May detect larger glycosylated bands ~50kDa. |
| Shipping Temperature: |
Blue Ice or 4ºC |
| Certificate of Analysis: |
0.5 µg/ml of SPC-505 was sufficient for detection of aquaporin 4 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody. |
| Cellular Localization: |
Membrane |
| References: |
1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396.
2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258.
3. Agre P., Nielsen S. (1996) Nephrologie. 17(7): 409-415.
4. Nagelhus E.A., Mathiisen T.M., Ottersen O.P. (2004) Neuroscience. 129(4): 905-913.
5. Lennon V.A., Kryzer T.J., Pittock S.J., Verkman A.S., Hinson S.R. (2005) J Exp Med. 202(4): 473-477.
6. Bradl M., Lassmann D.H. (2008) Int. MS Journal/MS Forum. 15(3): 75-78. |
| Launch Date: |
6/Dec/2011 |
| Tarriff Code: |
3002.15.0000 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| PubChem CID: |
27413198 |
|
| Product Detail: |
Aquaporin 5 functions as a water-specific channel and is expressed in secretory glands, lung and eye where it works in modulating the rate of fluid release. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 |
| Storage Temp: |
At least 12 months after purchase at 2 - 4 °C (lyophilized formulations). After reconstitution, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Rat; predicted to react with Human and Mouse due to sequence homology; |
| Immunogen: |
A synthetic peptide (PDEDWEEQREERKKT) corresponding to a region (245-259) from human Aquaporin 5. To enhance the immunological response, this peptide was coupled to carrier protein BSA. |
| Applications: |
WB; |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
Affinity purified on antigen column |
| Application Details: |
Western Blotting (WB). A concentration of 1.0 ug/ml is recommended for WB. Human Aquaporin 5 has a predicted length of 265 amino acids and MW of 28 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Alternative Names: |
Aquaporin-5; AQP-5; AQP5; |
| Accession Number: |
P55064 AQP5_HUMAN P47864 AQP5_RAT |
| Reconstitution: |
Reconstitute in 100 ul of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material. |
| Shelf Life: |
12 months after purchase |
| Specificity: |
The specificity of this antibody has been confirmed by WB against the antigen. | Rat; predicted to react with human and mouse due to sequence homology; |
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