| Product - please click for more details |
Brand |
Cat No |
Size |
Price (£) |
|
| HSP70 Antibody: FITC |
StressMarq |
SMC-113B-FITC |
200 µg |
£346.00
|
|
| Product Detail: |
HSP70 Antibody, Clone 1.86: FITC |
| Background Info: |
HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5).
All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com. |
| Product Type: |
Antibodies |
| Format: |
PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Storage Temp: |
-20ºC |
| Host Animal: |
Mouse |
| Species Reactivity: |
Hu | Ms | Rt | Bv | Pg |
| Immunogen: |
Bovine HSP70 |
| Applications: |
WB | ICC/IF | ELISA |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Clone number: |
1.86 |
| Antibody Isotype: |
IgG1 |
| Application Details: |
WB (1:500), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
| Target: |
HSP70 |
| Conjugate: |
FITC |
| Research Area(s): |
Cell Signaling | Protein Trafficking | Chaperone Proteins | Cancer | Tumor Biomarkers | Cancer | Heat Shock |
| Alternative Name(s): |
HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody |
| Category: |
Monoclonal |
| Immunogen Species: |
Bovine |
| Accession Number: |
NP_776975.1 |
| Gene ID: |
281825 |
| Swiss-Prot: |
Q27975 |
| Species Reactivity Full Name: |
Human | Mouse | Rat | Bovine | Pig |
| Purification: |
Protein G Purified |
| Concentration: |
1 mg/ml |
| Specificity: |
Detects 70kDa. Does not cross react with HSC70. |
| Shipping Temperature: |
Blue Ice or 4ºC |
| Certificate of Analysis: |
1 µg/ml of SMC-113 was sufficient for detection of HSP70 in 20 µg of Hela lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
| Cellular Localization: |
Cytoplasm |
| References: |
1. Welch W.J. and Suhan J.P. (1986) J Cell Biol. 103: 2035-2050.
2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J.Mol. Evol. 38(1): 1-17.
3. Rothman J. (1989) Cell 59: 591-601.
4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887.
5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad.Sci. USA 89: 7290-7294.
6. Fink A.L. (1999) Physiol. Rev. 79: 425-449.
|
| Launch Date: |
14-Apr-2012 |
| Tarriff Code: |
3002.10.0290 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| Cite this Product: |
StressMarq Biosciences Cat# SMC-113B-FITC, RRID: AB_2697984 |
|
| Ksr2 Antibody (pSer490): Alkaline Phosphatase |
StressMarq |
SPC-1004D-ALP |
100 µl |
£306.00
|
|
| Product Detail: |
Rabbit Anti-Human Ksr2 (pSer490) Polyclonal |
| Product Type: |
Antibodies |
| Format: |
PBS pH7.4, 50% glycerol, 0.025% Thimerosal |
| Storage Temp: |
-20ºC |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Hu |
| Immunogen: |
A phospho-specific peptide corresponding to residues surrounding Ser490 of human Ksr2 (AA487-493) |
| Applications: |
WB | AM |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
WB (1:250); optimal dilutions for assays should be determined by the user. |
| Target: |
Ksr2 (pSer490) |
| Conjugate: |
Alkaline Phosphatase |
| Research Area(s): |
Cell Signaling | Protein Phosphorylation | Serine / Threonine Kinases | MAPK Pathway |
| Alternative Name(s): |
FLJ25965 Antibody, hKSR 2 Antibody, Kinase suppressor of Ras 2 Antibody, KSR 2 Antibody, Ksr2 Antibody, KSR2_HUMAN Antibody
|
| Category: |
Polyclonal |
| Immunogen Species: |
Human |
| Accession Number: |
NP_775869 |
| Gene ID: |
283455 |
| Swiss-Prot: |
Q6VAB6 |
| Species Reactivity Full Name: |
Human |
| Purification: |
Peptide Affinity Purified |
| Specificity: |
Detects 107.632 kDa. |
| Shipping Temperature: |
Blue Ice or 4ºC |
| Certificate of Analysis: |
A 1:250 dilution of SPC-1004 was sufficient for detection of Ksr2 (pSer490) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
| Cellular Localization: |
Cytoplasm | Membrane |
| Tissue Specificity: |
Mainly expressed in brain and kidney. |
| Launch Date: |
29/Aug/2016 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| Cite this Product: |
StressMarq Biosciences Cat# SPC-1004D-ALP, RRID: AB_2708480 |
|
| MARCKS Antibody (pSer158): Streptavidin |
StressMarq |
SPC-1396D-STR |
100 µl |
£309.00
|
|
| Product Detail: |
Rabbit Anti-Human MARCKS (pSer158) Polyclonal |
| Background Info: |
MARCKS the most prominent cellular substrate of protein kinase C. Binds calmodulin, actin, and synapsin. A filamentous actin cross-linking protein. Critical for normal brain development. |
| Product Type: |
Antibodies |
| Format: |
PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide |
| Storage Temp: |
-20ºC |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Hu | Ms | Rt |
| Immunogen: |
Synthetic phospho-peptide from human MARCKS around the phosphorylation site of serine 158 (pSer158). |
| Applications: |
WB |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Antibody Isotype: |
IgG |
| Application Details: |
WB (1:250-1:1000); optimal dilutions for assays should be determined by the user. |
| Target: |
MARCKS (pSer158) |
| Conjugate: |
Streptavidin |
| Research Area(s): |
Cell Signaling | Protein Phosphorylation | Serine / Threonine Kinases | PKC | Neuroscience | Neurotransmission | Calcium Signaling | Calmodulin | Cardiovascular System | Cardiovascular Markers | Endothelial Cells | Cardiovascular System | Cardiovascular Markers | Smooth Muscle |
| Alternative Name(s): |
Protein kinase C substrate Antibody, MGC52672 Antibody, MACS Antibody, MARCS_HUMAN Antibody, 80K L Antibody, 80 kDa protein Antibody, protein kinase C substrate 80 kDa protein light chain Antibody, 80K L protein Antibody, PRKCSL Antibody, PKCSL Antibody, light chain Antibody, 81 kDa protein, 80K-L protein Antibody, Myristoylated alanine-rich C-kinase substrate Antibody, MARCKS Antibody, Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) Antibody, Phosphomyristin Antibody, 80 kDa protein, 80KL Antibody, myristoylated alanine rich C kinase substrate Antibody, Myristoylated alanine rich protein kinase C substrate Antibody, MARCS Antibody |
| Category: |
Polyclonal |
| Immunogen Species: |
Human |
| Accession Number: |
NP_002347.5 |
| Gene ID: |
4082 |
| Swiss-Prot: |
P29966 |
| Species Reactivity Full Name: |
Human | Mouse | Rat |
| Purification: |
Peptide Affinity Purified |
| Specificity: |
Detects endogenous levels of MARCKS only when phosphorylated at serine 158 (pSer158). Detects a band at ~. |
| Shipping Temperature: |
Blue Ice or 4ºC |
| Cellular Localization: |
Cytoskeleton | Membrane | Lipid-Anchor | Actin Cytoskeleton | Actin Filament Bundle | Centrosome | Extracellular Region Or Secreted | Extracellular Exosome | Nucleus | Germinal Vesicle | Plasma Membrane | Cell Cortex | Focal Adhesion |
| Launch Date: |
7/Mar/2018 |
| Tarriff Code: |
3002.15.0000 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| Cite this Product: |
Rabbit Anti-Human MARCKS (pSer158) Polyclonal (StressMarq Biosciences, Victoria BC, Cat# SPC-1396D-STR) |
|
| Rabbit polyclonal antibody to Aquaporin 5 (245-259): Affinity purified |
Biosensis |
R-1138-100 |
100 ug |
£270.00
|
|
| Product Detail: |
Aquaporin 5 functions as a water-specific channel and is expressed in secretory glands, lung and eye where it works in modulating the rate of fluid release. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 |
| Storage Temp: |
At least 12 months after purchase at 2 - 4 °C (lyophilized formulations). After reconstitution, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Rat; predicted to react with Human and Mouse due to sequence homology; |
| Immunogen: |
A synthetic peptide (PDEDWEEQREERKKT) corresponding to a region (245-259) from human Aquaporin 5. To enhance the immunological response, this peptide was coupled to carrier protein BSA. |
| Applications: |
WB; |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
Affinity purified on antigen column |
| Application Details: |
Western Blotting (WB). A concentration of 1.0 ug/ml is recommended for WB. Human Aquaporin 5 has a predicted length of 265 amino acids and MW of 28 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Alternative Names: |
Aquaporin-5; AQP-5; AQP5; |
| Accession Number: |
P55064 AQP5_HUMAN P47864 AQP5_RAT |
| Reconstitution: |
Reconstitute in 100 ul of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material. |
| Shelf Life: |
12 months after purchase |
| Specificity: |
The specificity of this antibody has been confirmed by WB against the antigen. | Rat; predicted to react with human and mouse due to sequence homology; |
|
