| Product - please click for more details |
Brand |
Cat No |
Size |
Price (£) |
|
| Mouse anti Mouse IgA, clone LO-MA-7 |
Monosan |
MON5050 |
1 ml |
£525.00
|
|
| Storage Temp: |
2-8°C |
| MONOSAN Brand Name: |
MONOSAN |
|
| Mouse anti Mouse IgA, Biotin, clone LO-MA-7 |
Monosan |
MON5050B |
1 ml |
£662.00
|
|
| Storage Temp: |
2-8°C |
| MONOSAN Brand Name: |
MONOSAN |
|
| Mouse anti Mouse IgA, Peroxidase, clone LO-MA-7 |
Monosan |
MON5050P |
1 ml |
£1220.00
|
|
| Storage Temp: |
2-8°C |
| MONOSAN Brand Name: |
MONOSAN |
|
| MaxSignal Flunixin ELISA Test Kit |
Bioo Scientific |
5050 |
1 x 96 wells |
£289.00
|
|
| Product Detail: |
Bioo Scientific offers test kits for the detection of regulated substances. Our Scientists have over 30 years of experience developing ELISA test kits. This allows us to produce high quality, dependable reagents with excellent reproducibility and sensitivity. Our expanding line of drug detection assays currently includes over 10 ELISA kits that can be used to screen over 20 drugs and/or their metabolites in a wide range of sample matrices. |
| Background Info: |
Bioo Scientific's enzyme-linked immunosorbent assay (ELISA) test kits are qualitative one-step kits designed as screening devices for the detection of drugs and/or their metabolites in forensic samples. |
| Product Type: |
Forensic Drug Detection |
| Additional Info: |
Intended for forensic toxicology samples only. It is recommended that all suspect samples be confirmed by a quantitative method such as GC/MS. |
| Application Details: |
Please use our contact form above to request a copy of the datasheet and pricing |
|
| MaxDiscovery Alkaline Phosphatase ELISA Kit |
Bioo Scientific |
5505-01 |
1 x 96 wells |
£513.00
|
|
| Product Detail: |
The MaxDiscovery Alkaline Phosphatase ELISA Kit is an enzyme immunoassay that analyzes the quantity of alkaline phosphatase in human cells, tissues, serum or urine. Alkaline phosphatase is a well known hydrolase enzyme responsible for removing phosphate groups from molecules including nucleotides, proteins and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the body, but is concentrated in the liver, bile duct, kidney, bone and placenta.
The normal range of alkaline phosphatase is 44-147 IU/L. High alkaline phosphatase levels can show that the bile ducts are blocked. Levels are significantly higher in children and pregnant women. Also, elevated alkaline phosphatase indicates that there could be active bone deposition occurring as alkaline phosphatase is a by-product of osteoblast activity.
Like most ELISA assays, the MaxDiscovery Alkaline Phosphatase ELISA Kit relies on a Horseradish Perioxidase (HRP) conjugated antibody and the TMB (3,3´,5,5´-tetramethylbenzidine) substrate. TMB is a chromogen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) that has major absorbances at 370 nm and 652 nm. The color then changes to yellow with the addition of acid with maximum absorbance at 450 nm. The relative amount of alkaline phosphatase protein in the cells will be directly proportional to the amount of signal that is obtained at 450 nm.
This kit contains materials for the extraction and quantitative detection of the alkaline phosphatase protein in cells, serum, tissues or urine. |
| Background Info: |
Benefits
>>> Ideal for the quantification of human alkaline phosphatase
>>> Highly sensitive and reproducible
>>> Fast and simple protocols
>>> Suited for automation
>>> Complete kits including coated plates
::
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans. |
| Product Type: |
Protein Detection - Reporter Assays |
| Additional Info: |
FORMAT: 1 x 96 well (Removable 8 wells x 12 strips)
MEASUREMENT: microtiter plate reader (450 nm)
TESTS PER KIT: The kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards and negative controls).
INCUBATION TIME: 2 hour and 15 minutes
SHELF LIFE: 6 months when stored under optimal conditions
SAMPLE TYPES: human cells, serum, urine and tissue
|
|
| Rhodopsin Antibody: ATTO 390 |
StressMarq |
SMC-176D-A390 |
100 µg |
£311.00
|
|
| Product Detail: |
Rhodopsin Antibody, Clone 4D2: ATTO 390 |
| Background Info: |
Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsins activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4).
Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5). |
| Product Type: |
Antibodies |
| Format: |
PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Storage Temp: |
-20ºC |
| Host Animal: |
Mouse |
| Species Reactivity: |
Mm | Fs | Av | Am | Shk |
| Immunogen: |
Bovine Rhodopsin |
| Applications: |
WB | IHC | ICC/IF | IP | ELISA |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Clone number: |
4D2 |
| Antibody Isotype: |
IgG1 |
| Application Details: |
WB (1:1000), IHC (1000); optimal dilutions for assays should be determined by the user. |
| Target: |
Rhodopsin |
| Conjugate: |
ATTO 390 |
| Research Area(s): |
Neuroscience | Neurotransmitter Receptors | Rhodopsins | Cell Signaling |
| Alternative Name(s): |
OPN2 Antibody, opsd Antibody, opsin 2 Antibody, opsin 2 rod pigment Antibody, opsin2 Antibody, RHO Antibody, RP4 Antibody, MGC138309 Antibody, Retinitis Pigmentosa 4 Antibody |
| Category: |
Monoclonal |
| Immunogen Species: |
Bovine |
| Accession Number: |
NP_001014890.1 |
| Gene ID: |
509933 |
| Swiss-Prot: |
P02699 |
| Species Reactivity Full Name: |
Mammals | Fish | Anchovies | Avian | Amphibians | Shark | Extinct Spiny Shark (Acanthodes bridgei) |
| Purification: |
Protein G Purified |
| Concentration: |
1 mg/ml |
| Specificity: |
Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates. |
| Shipping Temperature: |
Blue Ice or 4ºC |
| Certificate of Analysis: |
1 µg/ml of SMC-176 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Cellular Localization: |
Membrane |
| References: |
1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61.
2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867.
3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875.
4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742.
5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567. |
| Launch Date: |
29-Jun-2010 |
| Tarriff Code: |
3002.10.0290 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| Cite this Product: |
StressMarq Biosciences Cat# SMC-176D-A390, RRID: AB_2698806 |
|
| Ubiquitin Antibody |
StressMarq |
SPC-119A |
50 µl |
£133.00
|
|
| Product Detail: |
Rabbit Anti-Bovine Ubiquitin Polyclonal |
| Background Info: |
Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(3, 4). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (5). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as I?B? and p27 (6). |
| Product Type: |
Antibodies |
| Format: |
PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Storage Temp: |
-20ºC |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Hu | Ms | Rt | Bv | Mk | Hm | Rb | GP | Pg | Dg | Sh | Ck | Xe | Ys | Dr | Fs |
| Immunogen: |
Native bovine Ubiquitin, conjugated to KLH |
| Applications: |
WB | IHC | ICC/IF | IP |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
| Target: |
Ubiquitin |
| Conjugate: |
Unconjugated |
| Research Area(s): |
Cell Signaling | Post-translational Modifications | Ubiquitination |
| Alternative Name(s): |
Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody |
| Category: |
Polyclonal |
| Immunogen Species: |
Bovine |
| Accession Number: |
NP_776558.1 |
| Gene ID: |
281370 |
| Swiss-Prot: |
P0CG53 |
| Species Reactivity Full Name: |
Human | Mouse | Rat | Bovine | Monkey | Hamster | Rabbit | Guinea Pig (Cavia porcellus) | Pig | Dog | Sheep | Chicken | African clawed frog (Xenopus laevis) | Yeast | Fruit Fly (Drosophila melanogaster) | Fish | Rainbow Trout (Oncorhynchus mykiss) |
| Purification: |
Peptide Affinity Purified |
| Concentration: |
1 mg/ml |
| Specificity: |
Detects ~10kDa. It also recognizes ubiquinated proteins. |
| Shipping Temperature: |
Blue Ice or 4ºC |
| Certificate of Analysis: |
A 1:1000 dilution of SPC-119 was sufficient for detection of free ubiquitin in 15 µg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody. |
| Cellular Localization: |
Cell Membrane | Cytoplasm | Nucleus |
| References: |
1. Wilkinson K.D. (1995) Annu. Rev. Nutr. 15:161-189.
2. Smalle J., Vierstra R.D. (2004) Anu Rev Plant Biol. 55: 555-590.
3. Bonifacino J.S., et al. (1998) Annu Rev Cell Dev Biol. 14: 19-57.
4. Boston Biochem: "Ubiquitin Proteasome Pathway Overview http://www.bostonbiochem.com/upp.php
5. Yang M., et al. (1998) J Exp Med. 187: 1835-1846.
6. Chen Z.J., et al. (1996) Cell 84: 853-862. |
| Launch Date: |
1/Oct/2008 |
| Tarriff Code: |
3002.15.0000 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
|
| Abl1 Antibody (pTyr257): HRP |
StressMarq |
SPC-903D-HRP |
100 µl |
£300.00
|
|
| Product Detail: |
Rabbit Anti-Human Abl1 (pTyr257) Polyclonal |
| Background Info: |
ABL1 is a widely-expressed, non-receptor protein-tyrosine kinase. It is stabilized in the inactive form by an association between the SH3 domain and the SH2-TK linker region, interactions of the N-terminal cap, and contributions from an N-terminal myristoyl group and phospholipids. It is activated by autophosphorylation as well as by Src-family kinase-mediated phosphorylation. It can also be activated by RIN1 binding to the SH2 and SH3 domains. As it is stimulated by cell death inducers and DNA-damage, it is likely that the wild-type form of Abl1 is actually a tumour -suppressor protein. The normal active form of Abl1 is nuclear, and it is sequestered into the cytoplasm by interaction with 14-3-3 through Thr-735. It localizes to the mitochondria under conditions of oxidative stress. |
| Product Type: |
Antibodies |
| Format: |
PBS pH7.4, 50% glycerol, 0.025% Thimerosal |
| Storage Temp: |
-20ºC |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Hu |
| Immunogen: |
A phospho-specific peptide corresponding to residues surrounding Tyr257 of human Abl1 (AA254-260) |
| Applications: |
WB | AM |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
WB (1:250); optimal dilutions for assays should be determined by the user. |
| Target: |
Abl1 (pTyr257) |
| Conjugate: |
HRP |
| Research Area(s): |
Cell Signaling | Protein Phosphorylation | Tyrosine Kinases | Cell Signaling | Epigenetics and Nuclear Signaling | Transcription | Cancer | Tumor Biomarkers | Oncoproteins |
| Alternative Name(s): |
Abelson tyrosine protein kinase 1 Antibody, ABL Antibody, c ABL Antibody, p150 Antibody, bcr/abl Antibody |
| Category: |
Polyclonal |
| Immunogen Species: |
Human |
| Accession Number: |
NP_005148 |
| Gene ID: |
25 |
| Swiss-Prot: |
P00519 |
| Species Reactivity Full Name: |
Human |
| Purification: |
Peptide Affinity Purified |
| Specificity: |
Detects 122.945 kDa. |
| Shipping Temperature: |
Blue Ice or 4ºC |
| Certificate of Analysis: |
A 1:250 dilution of SPC-903 was sufficient for detection of Abl1 (pTyr257) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
| Cellular Localization: |
Cytoplasm | Cytoskeleton | Nucleus | Mitochondrion |
| Tissue Specificity: |
Widely expressed |
| Launch Date: |
29/Aug/2016 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| Cite this Product: |
StressMarq Biosciences Cat# SPC-903D-HRP, RRID: AB_2706666 |
|
| CDK6 Antibody (pTyr13): PE/ATTO 594 |
StressMarq |
SPC-944D-P594 |
100 µl |
£318.00
|
|
| Product Detail: |
Rabbit Anti-Human CDK6 (pTyr13) Polyclonal |
| Background Info: |
CDK6 is a protein-serine/threonine kinase. CDK6 is known to associate with cyclins D1, D2 and D3 and to be involved with the G1/S transition of the cell cycle. Multiple inhibitors of Cdk6 have been identified, including p18 and p19. These inhibitors bind to both free and complexed Cdk6, and they inhibit the activity of the cyclin D-bound Cdk6. |
| Product Type: |
Antibodies |
| Format: |
PBS pH7.4, 50% glycerol, 0.025% Thimerosal |
| Storage Temp: |
-20ºC |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Hu |
| Immunogen: |
A phospho-specific peptide corresponding to residues surrounding Tyr13 of human CDK6 (AA10-16) |
| Applications: |
WB | AM |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
WB (1:250); optimal dilutions for assays should be determined by the user. |
| Target: |
CDK6 (pTyr13) |
| Conjugate: |
PE/ATTO 594 |
| Research Area(s): |
Cancer | Cell Cycle | Cell Signaling | Epigenetics and Nuclear Signaling | Cell Signaling | Protein Phosphorylation | Cdks |
| Alternative Name(s): |
CDK6_HUMAN Antibody, Cell division protein kinase 6 Antibody, Crk 2 Antibody, Cyclin dependent kinase 6 Antibody, PLSTIRE Antibody, Serine/threonine protein kinase PLSTIRE Antibody, STQTL11 Antibody
|
| Category: |
Polyclonal |
| Immunogen Species: |
Human |
| Accession Number: |
NP_001250 |
| Gene ID: |
1021 |
| Swiss-Prot: |
Q00534 |
| Species Reactivity Full Name: |
Human |
| Purification: |
Peptide Affinity Purified |
| Specificity: |
Detects 36.938 kDa. |
| Shipping Temperature: |
Blue Ice or 4ºC |
| Certificate of Analysis: |
A 1:250 dilution of SPC-944 was sufficient for detection of CDK6 (pTyr13) in 10 µg of Jurkat cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
| Cellular Localization: |
Cytoplasmic |
| Launch Date: |
29/Aug/2016 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| Cite this Product: |
StressMarq Biosciences Cat# SPC-944D-P594, RRID: AB_2707405 |
|
| GW 441756 |
StressMarq |
SIH-450-50MG |
50 mg |
£619.00
|
|
| Product Detail: |
NGFR kinase inhibitor |
| Background Info: |
GW 441756 is a potent and selective NGF receptor tyrosine kinase A (TrkA) inhibitor. Specifically, it is shown to block apoptosis and cell death induced by TrkA. GW-441756 displays > 100-fold selectivity over a range of other kinases. |
| Product Type: |
Small Molecules |
| Storage Temp: |
-20ºC |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Research Area(s): |
Cell Signaling | Cancer | Apoptosis | Cancer Growth Inhibitors | Tyrosine Kinase Inhibitors |
| Alternative Name(s): |
1,3-Dihydro-3-[(1-methyl-1H-indol-3-yl)methylene]-2H-pyrrolo[3,2-b]pyridin-2-one |
| Category: |
Inhibitor |
| Shipping Temperature: |
Shipped Ambient |
| References: |
1. Wood E.R., et al. (2004) Bioorganic & Med. Chem. Ltr. 14(4): 953957.
2. Jung E.J., Kim C.W., & Kim D.R. (2008) Exp. Mol. Med. 40(3): 276285. |
| Launch Date: |
1-Oct-2014 |
| Tarriff Code: |
3822.00.1090 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| CAS No.: |
504433-23-2 |
| MolecularFormula: |
C17H13N3O |
| Solubility: |
Soluble to 15 mM in DMSO |
| Appearance: |
Red solid |
| Safety Phrases: |
Classification:
Not a hazardous substance or mixture.
Safety Phrases:
S22 - Do not breathe dust.
S24/25 - Avoid contact with skin and eyes.
S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. |
| Cite this Product: |
GW 441756 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-450-50MG) |
| PubChem CID: |
9943465 |
|
| AtpA | Alpha subunit of ATP synthase, chloroplastic |
Agrisera |
AS08 304 |
50 µl |
£352.00
|
|
| Background Info: |
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized |
| Storage Temp: |
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana, Capsicum annuum, Heliobacterium modesticaldum, Zea mays |
| Expected Species: |
Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Cyanobacteria |
| Immunogen: |
Recombinant maize chloroplast AtpA P05022 |
| Applications: |
Immunoprecipitation (IP), Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
1 : 5 000 (WB) |
| Purity: |
Serum |
| Reconstitution: |
For reconstitution add 100 µl of sterile water. |
| Related products: |
AS03 030 | anti-ATP synthase subunit beta hen antibodyAS08 312 | anti-ATP synthase subunit gamma antibodyAS05 071 | anti-ATP synthase subunit c antibodyAS08 370 | anti-ATP synthase whole enzyme |AgriseraSuperDeal |
| Expected | apparent MW: |
55 kDa |
| Not reactive in: |
No confirmed exceptions from predicted reactivity are currently known. |
| Selected references: |
Zhang et al. (2017). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989. Jeon et al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y. Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016. Camejo et al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370. Yang et al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006. |
| Special application note: |
Sequence of the protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757 |
| UniProt number: |
P05022 |
| TAIR number: |
ATCG00120 |
|
| UBQ11 | Ubiquitin |
Agrisera |
AS08 307 |
50 µl |
£270.00
|
|
| Background Info: |
Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS). |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized |
| Storage Temp: |
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana, Hordeum vulgare, Nicotiana benthamina, Solanum lycopersicum |
| Expected Species: |
Brachypodium distachyon, Brassica napus, Capsella rubella, Cochliobolus heterostrophus, Citrus clementina, Citrus sinensis, Coffea canephora, Cymbidium faberi, Eucalyptus grandis, Erythrane guttata, Glycine max, Glycine soja, Ipomoea batatas, Medicago truncatula, Magnaporthe oryzae, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Populus trichocarpa, Prunus persica, Pyrus communis, Ricinus communis, Solanum nigrum, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Theobroma cacao, Zea mays , Vitis vinifera |
| Immunogen: |
Recombinant Arabidopsis thaliana ubq11, with N-terminal His-tag; UniProt:P0CH33, TAIR: AT4G05050 |
| Applications: |
Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
Antibody can be used to check qubiquitination status in the whole plant extracts. |
| Application Details: |
1 : 10 000 (WB) |
| Purity: |
Serum |
| Reconstitution: |
For reconstitution add 50 µl of sterile water |
| Related products: |
AS10 307A | antt-UBQ11 | Ubiquitin, affinity purified, rabbit antibodyAS10 307S | UBQ11 | Ubiquitin protein | positive control |AgriseraSuperDeal |
| Expected | apparent MW: |
8.5 kDa |
| Not reactive in: |
Algae |
| Selected references: |
Witzel et al. (2017). A Proteomic Approach Suggests Unbalanced Proteasome Functioning Induced by the Growth-Promoting Bacterium Kosakonia radicincitans in Arabidopsis. Front Plant Sci. 2017 Apr 26;8:661. doi: 10.3389/fpls.2017.00661. Gorovits et al. (2017). The six Tomato yellow leaf curl virus genes expressed individually in tomato induce different levels of plant stress response attenuation. Cell Stress Chaperones. 2017 Mar 21. doi: 10.1007/s12192-017-0766-0. Moshe at al. (2015). Tomato plant cell death induced by inhibition of HSP90 is alleviated by Tomato yellow leaf curl virus infection. Mol Plant Pathol. 2015 May 12. doi: 10.1111/mpp.12275. Hamorsky et al. (2015). N-Glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential. Sci Rep. 2015 Jan 23;5:8003. doi: 10.1038/srep08003. Kong et al. (2014). Quantitative proteomics analysis reveals that the nuclear cap-binding complex proteins Arabidopsis CBP20 and CBP80 modulate the salt stress response. J Proteome Res. 2014 Apr 1. Zulet et al. (2013). Proteolytic Pathways Induced by Herbicides That Inhibit Amino Acid Biosynthesis. PLoS ONE 8(9): e73847. doi:10.1371/journal.pone.0073847. (Pisum sativum, western blot)Ferrández-Ayela et al. (2013). Arabidopsis TRANSCURVATA1 Encodes NUP58, a Component of the Nucleopore Central Channel. PLOS ONE, June 2013. Ustun et al. (2013). The Xanthomonas campestris Type III Effector XopJ Targets the Host Cell Proteasome to Suppress Salicylic-Acid Mediated Plant Defence. PLOS Pathog. June 9. |
| UniProt number: |
P0CH33 |
| TAIR number: |
AT4G05050 |
|
| UBQ11 | Ubiquitin (affinity purified) |
Agrisera |
AS08 307A |
50 µg |
£270.00
|
|
| Background Info: |
Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS). |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized |
| Storage Temp: |
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana, Hordeum vulgare, Lupinus angustifolius, Malus domestica, Nicotiana benthamina, Oryza sativa, Pisum sativum, Solanum tuberosum, Zea mays |
| Expected Species: |
Brachypodium distachyon, Brassica napus, Capsella rubella, Cochliobolus heterostrophus, Citrus clementina, Citrus sinensis, Coffea canephora, Cymbidium faberi, Eucalyptus grandis, Erythrane guttata, Glycine max, Glycine soja, Ipomoea batatas, Medicago truncatula, Magnaporthe oryzae, Nicotiana tabacum, Phaseolus vulgaris, Populus trichocarpa, Prunus persica, Pyrus communis, Ricinus communis, Solanum nigrum, Sorghum bicolor, Triticum aestivum, Theobroma cacao, Vitis vinifera |
| Immunogen: |
Recombinant Arabidopsis thaliana ubiquitin, ubq11, with N-terminal His-tag; TAIR: At4g05050 |
| Applications: |
Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
Antibody can be used to check qubiquitination status in the whole plant extracts.Technical note: It is very difficult to detect ubiquitin monomers in total cell extracts due to a great abundance of poly and multi-ubiquitinated proteins. Recommended is size separation of protein extracts before gel electrophoresis focused on good resolution of region between 6-10 kDa. |
| Application Details: |
1 : 10 000 (WB) |
| Purity: |
Affinity purified serum in PBS pH 7.4. |
| Reconstitution: |
For reconstitution add 50 µl of sterile water |
| Related products: |
AS08 307 | UBQ11 | Ubiquitin, rabbit antibody, provided in a serum formatAS10 307S | UBQ11 | Ubiquitin protein | positive control |AgriseraSuperDeal |
| Expected | apparent MW: |
8.5 kDa |
| Not reactive in: |
Algae |
| Selected references: |
Krasuska et al. (2016). Nitric oxide-polyamines cross-talk during dormancy release and germination of apple embryos. Nitric Oxide. 2016 Nov 23. pii: S1089-8603(16)30178-1. doi: 10.1016/j.niox.2016.11.003. [Epub ahead of print] |
| TAIR number: |
AT4G05050 |
|
| SnRK2.2, SnRK2.3, SnRK2.6 | Ser/Thr-protein kinase SnRK |
Agrisera |
AS14 2783 |
50 µg |
£270.00
|
|
| Background Info: |
SnRK2.2, SnRK2.3, SnRK2.6 are members of SNF1-related protein kinases whose activity is activated by ionic (salt) and non-ionic (mannitol) osmotic stress. Involved in ABA signalling pathway. Alternative names: OST1-kinase-like 2, Protein ATHPROKIN B, SNF1-related kinase 2.3, SnRK2.3, Protein OPEN STOMATA 1SNF1-related kinase 2.6, SnRK2.6, Serine/threonine-protein kinase OST1, OST1-kinase-like 3, Protein ATHPROKIN A, SNF1-related kinase 2.2, SnRK2.2 |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized in PBS pH 7.4 |
| Storage Temp: |
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana |
| Expected Species: |
Brassica oleracea, Glycine max, Medicago sativa, Morsu notabilis, Oryza sativa, Phaseolus vulgaris, Populus sp., Triticum sp., Vicia faba |
| Immunogen: |
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SnRK2.2 UniProt:Q39192 ,TAIR: At3g50500, SnRK2.3, UniProt: Q39193 TAIR: At5g66880, SnRK2.6 UniProt: Q940H6, TAIR: At4g33950 |
| Applications: |
Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
1 : 1000 (WB) |
| Purity: |
Affinity purified serum in PBS, pH 7.4 |
| Reconstitution: |
For reconstitution add 50 µl of sterile water. |
| Related products: |
AS13 2635 | SRK2E | Ser/Thr-protein kinase SnRK2.6 AS12 1855 | anti-S6K1/2 | Ribosomal S6 kinase 1/2, rabbit antibodiesAS13 2664 | anti-S6K1-2 | Ribosomal-protein S6 kinase homolog 1,2 - phosphorylated, rabbit antibodiesPlant protein extraction buffer Secondary antibodies |AgriseraSuperDeal |
| Expected | apparent MW: |
41 kDa |
| Not reactive in: |
Chlamydomonas reinhardtii |
| Selected references: |
to be added when available, antibody released in September 2015 |
| UniProt number: |
Q940H6 , Q39193 , Q39192 |
| TAIR number: |
At3g50500,At4g33950,At5g66880 |
|
| Mouse polyclonal antibody to human Protocadherin alpha-2: whole serum |
Biosensis |
Mo-950-50 |
50 ul |
£270.00
|
|
| Product Detail: |
Protocadherin alpha-2 is 1 of 15 tandemly arranged genes within the PCDHA gene cluster. These 15 N-terminal 'variable' exons are individually spliced to a downstream C-terminal constant region to form distinct PCDHA transcripts. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain.The PCDH proteins are cadherin-like cell surface proteins that are expressed in neurons and are present at synaptic junctions. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Liquid. 50% glycerol |
| Storage Temp: |
Keep aliquots at -20 °C for higher stability or at 4 °C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. |
| Host Animal: |
Mouse |
| Species Reactivity: |
Human. Other species have not been tested. |
| Immunogen: |
Partial recombinant human PCDH-alpha-2 (189-282) with a GST tag. |
| Applications: |
WB;ELISA |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
Whole serum |
| Antibody Isotype: |
Mixed |
| Application Details: |
This antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Alternative Names: |
PCDH-alpha-2; PCDHA2 |
| Accession Number: |
PCDA2_HUMAN |
| Shelf Life: |
12 months after purchase |
| Specificity: |
Specificity has been confirmed by WB and direct ELISA against the antigen. | Human. Other species have not been tested. |
|
