| Product - please click for more details |
Brand |
Cat No |
Size |
Price (£) |
|
| Mouse anti Mouse IgA, clone LO-MA-7 |
Monosan |
MON5050 |
1 ml |
£525.00
|
|
| Storage Temp: |
2-8°C |
| MONOSAN Brand Name: |
MONOSAN |
|
| Mouse anti Mouse IgA, Biotin, clone LO-MA-7 |
Monosan |
MON5050B |
1 ml |
£662.00
|
|
| Storage Temp: |
2-8°C |
| MONOSAN Brand Name: |
MONOSAN |
|
| Mouse anti Mouse IgA, Peroxidase, clone LO-MA-7 |
Monosan |
MON5050P |
1 ml |
£1220.00
|
|
| Storage Temp: |
2-8°C |
| MONOSAN Brand Name: |
MONOSAN |
|
| MaxSignal Flunixin ELISA Test Kit |
Bioo Scientific |
5050 |
1 x 96 wells |
£289.00
|
|
| Product Detail: |
Bioo Scientific offers test kits for the detection of regulated substances. Our Scientists have over 30 years of experience developing ELISA test kits. This allows us to produce high quality, dependable reagents with excellent reproducibility and sensitivity. Our expanding line of drug detection assays currently includes over 10 ELISA kits that can be used to screen over 20 drugs and/or their metabolites in a wide range of sample matrices. |
| Background Info: |
Bioo Scientific's enzyme-linked immunosorbent assay (ELISA) test kits are qualitative one-step kits designed as screening devices for the detection of drugs and/or their metabolites in forensic samples. |
| Product Type: |
Forensic Drug Detection |
| Additional Info: |
Intended for forensic toxicology samples only. It is recommended that all suspect samples be confirmed by a quantitative method such as GC/MS. |
| Application Details: |
Please use our contact form above to request a copy of the datasheet and pricing |
|
| MaxDiscovery Alkaline Phosphatase ELISA Kit |
Bioo Scientific |
5505-01 |
1 x 96 wells |
£513.00
|
|
| Product Detail: |
The MaxDiscovery Alkaline Phosphatase ELISA Kit is an enzyme immunoassay that analyzes the quantity of alkaline phosphatase in human cells, tissues, serum or urine. Alkaline phosphatase is a well known hydrolase enzyme responsible for removing phosphate groups from molecules including nucleotides, proteins and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the body, but is concentrated in the liver, bile duct, kidney, bone and placenta.
The normal range of alkaline phosphatase is 44-147 IU/L. High alkaline phosphatase levels can show that the bile ducts are blocked. Levels are significantly higher in children and pregnant women. Also, elevated alkaline phosphatase indicates that there could be active bone deposition occurring as alkaline phosphatase is a by-product of osteoblast activity.
Like most ELISA assays, the MaxDiscovery Alkaline Phosphatase ELISA Kit relies on a Horseradish Perioxidase (HRP) conjugated antibody and the TMB (3,3´,5,5´-tetramethylbenzidine) substrate. TMB is a chromogen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) that has major absorbances at 370 nm and 652 nm. The color then changes to yellow with the addition of acid with maximum absorbance at 450 nm. The relative amount of alkaline phosphatase protein in the cells will be directly proportional to the amount of signal that is obtained at 450 nm.
This kit contains materials for the extraction and quantitative detection of the alkaline phosphatase protein in cells, serum, tissues or urine. |
| Background Info: |
Benefits
>>> Ideal for the quantification of human alkaline phosphatase
>>> Highly sensitive and reproducible
>>> Fast and simple protocols
>>> Suited for automation
>>> Complete kits including coated plates
::
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans. |
| Product Type: |
Protein Detection - Reporter Assays |
| Additional Info: |
FORMAT: 1 x 96 well (Removable 8 wells x 12 strips)
MEASUREMENT: microtiter plate reader (450 nm)
TESTS PER KIT: The kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards and negative controls).
INCUBATION TIME: 2 hour and 15 minutes
SHELF LIFE: 6 months when stored under optimal conditions
SAMPLE TYPES: human cells, serum, urine and tissue
|
|
| SOD1 (UbetaB) Antibody: ATTO 594 |
StressMarq |
SPC-205D-A594 |
100 µg |
£291.00
|
|
| Product Detail: |
Rabbit Anti-Human SOD1 (UbetaB) Polyclonal |
| Background Info: |
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (2). It works by catalyzing the dismutation of the superoxide radical O2? to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). In general, SODs play a major role in antioxidant defense mechanisms (3). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (2). Misfolding of SOD1 has been implicated in Amyotrophic lateral sclerosis (ALS). Therefore conformation specific antibodies such as Anti-SOD1 (UbetaB), which targets an unfolded region of the beta barrel of SOD1, are useful for determining the conformation of SOD1 in affected tissues (5). This antibody can be used in conjunction with Anti-SOD1 (EDI) (SPC-206D) which detects an exposed dimer interface (EDI) of SOD1.
|
| Product Type: |
Antibodies |
| Format: |
PBS, 50% glycerol, 0.09% sodium azide |
| Storage Temp: |
-20ºC |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Hu | Ms | Rt |
| Immunogen: |
N-terminal region of SOD1, unfolded beta barrel region |
| Applications: |
WB | ICC/IF |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
| Target: |
SOD1 |
| Conjugate: |
ATTO 594 |
| Research Area(s): |
Cancer | Oxidative Stress | Neuroscience | Neurodegeneration | ALS Disease |
| Alternative Name(s): |
USOD Antibody, Superoxide dismutase 1 Antibody, SOD Antibody, SOD1 unfolded beta barrel region Antibody |
| Category: |
Polyclonal |
| Immunogen Species: |
Human |
| Accession Number: |
CAG46542 |
| Gene ID: |
6647 |
| Swiss-Prot: |
P00441 |
| Species Reactivity Full Name: |
Human | Mouse | Rat |
| Purification: |
Protein A Purified |
| Concentration: |
1 mg/ml |
| Specificity: |
Recognizes a conformation specific epitope where the beta barrel is unfolded. |
| Shipping Temperature: |
Blue Ice or 4ºC |
| Certificate of Analysis: |
1µg/ml of SPC-205 was sufficient for detection of the unfolded beta barrel of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody. |
| Cellular Localization: |
Cytoplasm |
| References: |
1. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180.
2. Furukawa Y., and OHalloran T. (2006) Antioxid Redox Signal. 8(5-6):847-67.
3. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol. 284:L917-L925.
4. Hassan H.M. (1988) Free Radical Biol. Med. 5:377-385.
5. Kerman A., et al. (2010) Acta Neuropathol. 119:335-344.
|
| Launch Date: |
25/Sep/2013 |
| Tarriff Code: |
3002.15.0000 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| PubChem CID: |
28057013 |
|
| c-Jun Antibody (pThr91): PerCP |
StressMarq |
SPC-1363D-PCP |
100 µl |
£310.00
|
|
| Product Detail: |
Rabbit Anti-Human c-Jun (pThr91) Polyclonal |
| Background Info: |
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. |
| Product Type: |
Antibodies |
| Format: |
PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide |
| Storage Temp: |
-20ºC |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Hu | Ms | Rt |
| Immunogen: |
Synthetic phospho-peptide from human c-Jun around the phosphorylation site of threonine 91 (pThr91). |
| Applications: |
WB | IHC |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Antibody Isotype: |
IgG |
| Application Details: |
WB (1:250-1:1000); IHC (1:25-1:100); optimal dilutions for assays should be determined by the user. |
| Target: |
c-Jun (pThr91) |
| Conjugate: |
PerCP |
| Research Area(s): |
Cell Signaling | Epigenetics and Nuclear Signaling | Transcription | Domain Families | Hlh | Cell Signaling | Epigenetics and Nuclear Signaling | Nuclear Signaling Pathways | SMADs | Cell Signaling | Epigenetics and Nuclear Signaling | Transcription | Transcription Factors | Cancer | Tumor Biomarkers | Oncoproteins | Oncoprotein Transcription Factors | Immunology | Innate Immunity | TLR Signaling |
| Alternative Name(s): |
V jun sarcoma virus 17 oncogene homolog (avian) Antibody, Proto oncogene c jun Antibody, Jun Activation Domain Binding Protein Antibody, Activator protein 1 Antibody, V-jun avian sarcoma virus 17 oncogene homolog Antibody, AP 1 Antibody, Transcription Factor AP 1 Antibody, Transcription factor AP-1 Antibody, JUN protein Antibody, cJun Antibody, vJun Avian Sarcoma Virus 17 Oncogene Homolog Antibody, Proto oncogene cJun Antibody, Enhancer Binding Protein AP1 Antibody, Proto-oncogene c-jun Antibody, V jun sarcoma virus 17 oncogene homolog Antibody, p39 Antibody, Transcription Factor AP1 Antibody, JUN_HUMAN Antibody, V jun avian sarcoma virus 17 oncogene homolog Antibody, Oncogene JUN Antibody, JUN Antibody, Jun proto oncogene Antibody, JUNC Antibody, Jun oncogene Antibody, AP1 Antibody |
| Category: |
Polyclonal |
| Immunogen Species: |
Human |
| Accession Number: |
NP_002219.1 |
| Gene ID: |
3725 |
| Swiss-Prot: |
P05412 |
| Species Reactivity Full Name: |
Human | Mouse | Rat |
| Purification: |
Peptide Affinity Purified |
| Specificity: |
Detects endogenous levels of c-Jun only when phosphorylated at threonine 91 (pThr91). Detects a band at ~48 kDa. |
| Shipping Temperature: |
Blue Ice or 4ºC |
| Cellular Localization: |
Nucleus | Cytosol | Nuclear Chromosome | Nuclear Euchromatin | Nucleoplasm | Transcription Factor Ap-1 Complex | Transcriptional Repressor Complex |
| Launch Date: |
7/Mar/2018 |
| Tarriff Code: |
3002.15.0000 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| Cite this Product: |
Rabbit Anti-Human c-Jun (pThr91) Polyclonal (StressMarq Biosciences, Victoria BC, Cat# SPC-1363D-PCP) |
|
| GW 441756 |
StressMarq |
SIH-450-50MG |
50 mg |
£619.00
|
|
| Product Detail: |
NGFR kinase inhibitor |
| Background Info: |
GW 441756 is a potent and selective NGF receptor tyrosine kinase A (TrkA) inhibitor. Specifically, it is shown to block apoptosis and cell death induced by TrkA. GW-441756 displays > 100-fold selectivity over a range of other kinases. |
| Product Type: |
Small Molecules |
| Storage Temp: |
-20ºC |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Research Area(s): |
Cell Signaling | Cancer | Apoptosis | Cancer Growth Inhibitors | Tyrosine Kinase Inhibitors |
| Alternative Name(s): |
1,3-Dihydro-3-[(1-methyl-1H-indol-3-yl)methylene]-2H-pyrrolo[3,2-b]pyridin-2-one |
| Category: |
Inhibitor |
| Shipping Temperature: |
Shipped Ambient |
| References: |
1. Wood E.R., et al. (2004) Bioorganic & Med. Chem. Ltr. 14(4): 953957.
2. Jung E.J., Kim C.W., & Kim D.R. (2008) Exp. Mol. Med. 40(3): 276285. |
| Launch Date: |
1-Oct-2014 |
| Tarriff Code: |
3822.00.1090 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| CAS No.: |
504433-23-2 |
| MolecularFormula: |
C17H13N3O |
| Solubility: |
Soluble to 15 mM in DMSO |
| Appearance: |
Red solid |
| Safety Phrases: |
Classification:
Not a hazardous substance or mixture.
Safety Phrases:
S22 - Do not breathe dust.
S24/25 - Avoid contact with skin and eyes.
S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. |
| Cite this Product: |
GW 441756 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-450-50MG) |
| PubChem CID: |
9943465 |
|
| HO-1 ELISA Kit |
StressMarq |
SKT-111-96 |
96 well |
£501.00
|
|
| Product Detail: |
Colorimetric detection of heme oxygenase 1 |
| Background Info: |
Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2).
There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4). |
| Product Type: |
ELISA Kits |
| Storage Temp: |
4ºC |
| Species Reactivity: |
Hu | Rt |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Target: |
HO-1 |
| Research Area(s): |
Cancer | Oxidative Stress |
| Alternative Name(s): |
Heme oxygenase 1 ELISA Kit, Hemox ELISA Kit, HMOX1 ELISA Kit, HO1 ELISA Kit, HO 1 ELISA Kit, HSP32 ELISA Kit |
| Category: |
Assay Kits |
| Species Reactivity Full Name: |
Human | Rat |
| Shipping Temperature: |
Blue Ice |
| References: |
1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86.
2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47.
3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411419.
4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8.
5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 1092510930.
6. Yet S.F., et al. (2003) FASEB J. 17: 17591761.
7. Yachie A., et al. (1999) J Clin Invest. 103: 129135. |
| Launch Date: |
1/Dec/2011 |
| Tarriff Code: |
3822.00.1090 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| Assay Type: |
Sandwich ELISA (Enzyme-linked Immunosorbent Assay) |
| Assay Type Abbreviation: |
Sandwich |
| Platform: |
Microplate |
| Utility: |
ELISA kit used to quantitate HO-1 concentration in samples. |
| Assay Overview: |
1. Prepare Standard and samples in Standard and Sample Diluent.
2. Add 50 µL of Pre-Treatment Buffer to all sample and standard wells.
3. Add 50 µL of Standard and sample to appropriate wells.
4. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 2 hours.
5. Wash plate four times with 1X Wash Buffer.
6. Add 100 µL of Biotinylated Antibody Working Solution to each well.
7. Cover plate with Plate Sealer and incubate at room temperature for 1 hour.
8. Wash plate four times with 1X Wash Buffer as described in step 5.
9. Add 100 µL of Streptavidin-HRP Working Solution to each well.
10. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes.
11. Wash plate four times with 1X Wash Buffer as described in step 5.
12. Add 100 µL of TMB Substrate to each well.
13. Develop the plate in the dark at room temperature for 30 minutes.
14. Stop reaction by adding 100 µL of Stop Solution to each well.
15. Measure absorbance on a plate reader at 450 nm. |
| Kit Components: |
Anti-HO-1 Immunoassay Plate | 5X HO-1 Extraction Reagent | Recombinant HO-1 Standard | Standard and Sample Diluent | 10X Wash Buffer Concentrate | Anti-HO-1 Biotinylated Antibody Concentrate | Anti-HO-1 Biotinylated Antibody Diluent | Streptavidin: HRP Concentrate | Streptavidin: HRP Diluent | TMB Substrate | Stop Solution | Pre-treatment Buffer |
| Sample Type(s): |
Cell Lysates | Tissue | Serum |
| Number of Samples: |
40 samples in duplicate |
| Incubation Time: |
30 minutes |
| Sensitivity: |
0.21 ng/ml |
| Assay Range: |
0.781 - 50 ng/ml |
| Cite this Product: |
HO-1 ELISA Kit (StressMarq Biosciences, Canada, Cat # SKT-111-480) |
| PubChem CID: |
28535981 |
| Trademark: |
StressXpress® |
|
| AtpA | Alpha subunit of ATP synthase, chloroplastic |
Agrisera |
AS08 304 |
50 µl |
£351.00
|
|
| Background Info: |
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Storage Temp: |
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana, Capsicum annuum, Heliobacterium modesticaldum, Zea mays |
| Expected Species: |
Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Cyanobacteria |
| Immunogen: |
Recombinant maize chloroplast AtpA P05022 |
| Applications: |
Immunoprecipitation (IP), Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
1 : 5 000 (WB) |
| Purity: |
Serum |
| Format: |
Lyophilized |
| Reconstitution: |
For reconstitution add 100 µl of sterile water. |
| Related products: |
AS03 030 | anti-ATP synthase subunit beta hen antibodyAS08 312 | anti-ATP synthase subunit gamma antibodyAS05 071 | anti-ATP synthase subunit c antibodyAS08 370 | anti-ATP synthase whole enzyme |
| Expected | apparent MW: |
55 kDa |
| Not reactive in: |
No confirmed exceptions from predicted reactivity are currently known. |
| Selected references: |
Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016. Camejo et al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370. Yang et al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006. |
| Special application note: |
Sequence of the protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757 |
| UniProt number: |
P05022 |
| TAIR number: |
ATCG00120 |
|
| UBQ11 | Ubiquitin |
Agrisera |
AS08 307 |
50 µl |
£270.00
|
|
| Background Info: |
Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS). |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Storage Temp: |
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana, Hordeum vulgare, Nicotiana benthamina, Solanum lycopersicum |
| Expected Species: |
Brachypodium distachyon, Brassica napus, Capsella rubella, Cochliobolus heterostrophus, Citrus clementina, Citrus sinensis, Coffea canephora, Cymbidium faberi, Eucalyptus grandis, Erythrane guttata, Glycine max, Glycine soja, Ipomoea batatas, Medicago truncatula, Magnaporthe oryzae, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Populus trichocarpa, Prunus persica, Pyrus communis, Ricinus communis, Solanum nigrum, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Theobroma cacao, Zea mays , Vitis vinifera |
| Immunogen: |
Recombinant Arabidopsis thaliana ubq11, with N-terminal His-tag; UniProt:P0CH33, TAIR: AT4G05050 |
| Applications: |
Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
Antibody can be used to check qubiquitination status in the whole plant extracts. |
| Application Details: |
1 : 10 000 (WB) |
| Purity: |
Serum |
| Format: |
Lyophilized |
| Reconstitution: |
For reconstitution add 50 µl of sterile water |
| Related products: |
AS10 307A | antt-UBQ11 | Ubiquitin, affinity purified, rabbit antibodyAS10 307S | UBQ11 | Ubiquitin protein | positive control |
| Expected | apparent MW: |
8.5 kDa |
| Not reactive in: |
Algae |
| Selected references: |
Witzel et al. (2017). A Proteomic Approach Suggests Unbalanced Proteasome Functioning Induced by the Growth-Promoting Bacterium Kosakonia radicincitans in Arabidopsis. Front Plant Sci. 2017 Apr 26;8:661. doi: 10.3389/fpls.2017.00661. Gorovits et al. (2017). The six Tomato yellow leaf curl virus genes expressed individually in tomato induce different levels of plant stress response attenuation. Cell Stress Chaperones. 2017 Mar 21. doi: 10.1007/s12192-017-0766-0. Moshe at al. (2015). Tomato plant cell death induced by inhibition of HSP90 is alleviated by Tomato yellow leaf curl virus infection. Mol Plant Pathol. 2015 May 12. doi: 10.1111/mpp.12275. Hamorsky et al. (2015). N-Glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential. Sci Rep. 2015 Jan 23;5:8003. doi: 10.1038/srep08003. Kong et al. (2014). Quantitative proteomics analysis reveals that the nuclear cap-binding complex proteins Arabidopsis CBP20 and CBP80 modulate the salt stress response. J Proteome Res. 2014 Apr 1. Zulet et al. (2013). Proteolytic Pathways Induced by Herbicides That Inhibit Amino Acid Biosynthesis. PLoS ONE 8(9): e73847. doi:10.1371/journal.pone.0073847. (Pisum sativum, western blot)Ferrández-Ayela et al. (2013). Arabidopsis TRANSCURVATA1 Encodes NUP58, a Component of the Nucleopore Central Channel. PLOS ONE, June 2013. Ustun et al. (2013). The Xanthomonas campestris Type III Effector XopJ Targets the Host Cell Proteasome to Suppress Salicylic-Acid Mediated Plant Defence. PLOS Pathog. June 9. |
| UniProt number: |
P0CH33 |
| TAIR number: |
AT4G05050 |
|
| UBQ11 | Ubiquitin (affinity purified) |
Agrisera |
AS08 307A |
50 µg |
£270.00
|
|
| Background Info: |
Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS). |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Storage Temp: |
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana, Malus domestica, Nicotiana benthamina, Oryza sativa |
| Expected Species: |
Brachypodium distachyon, Brassica napus, Capsella rubella, Cochliobolus heterostrophus, Citrus clementina, Citrus sinensis, Coffea canephora, Cymbidium faberi, Eucalyptus grandis, Erythrane guttata, Glycine max, Glycine soja, Ipomoea batatas, Medicago truncatula, Magnaporthe oryzae, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Populus trichocarpa, Prunus persica, Pyrus communis, Ricinus communis, Solanum nigrum, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Theobroma cacao, Zea mays , Vitis vinifera |
| Immunogen: |
Recombinant Arabidopsis thaliana ubiquitin, ubq11, with N-terminal His-tag; TAIR: At4g05050 |
| Applications: |
Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
Antibody can be used to check qubiquitination status in the whole plant extracts. |
| Application Details: |
1 : 10 000 (WB) |
| Purity: |
Affinity purified serum |
| Format: |
Lyophilized |
| Reconstitution: |
For reconstitution add 50 µl of sterile water |
| Related products: |
AS08 307 | UBQ11 | Ubiquitin, rabbit antibody, provided in a serum formatAS10 307S | UBQ11 | Ubiquitin protein | positive control |
| Expected | apparent MW: |
8.5 kDa |
| Not reactive in: |
Algae |
| Selected references: |
Krasuska et al. (2016). Nitric oxide-polyamines cross-talk during dormancy release and germination of apple embryos. Nitric Oxide. 2016 Nov 23. pii: S1089-8603(16)30178-1. doi: 10.1016/j.niox.2016.11.003. [Epub ahead of print] |
| TAIR number: |
AT4G05050 |
|
| UBQ11 | Ubiquitin protein (positive control) |
Agrisera |
AS10 307S |
50 µl |
£98.00
|
|
| Background Info: |
Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS).Source of positive control, ubiquitin protein: full length recombinant Arabidopsis thaliana ubiquitin, ubq11, with N-terminal 1XHis-3XHA-tag; locus accession: At4g05050 |
| Product Type: |
Antibody |
| Storage Temp: |
Store protein standard at -20°C; Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes. |
| Applications: |
Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
Concentration: 0.8 µg/µl. Recommended load per gel to allow Coomasie staining and western blot detection is 2.5-5 µl.1x SDS Loading Buffer contains:50 mM Tris-HCl pH 6.82% SDS10% glycerol1% β-mercaptoethanol12.5 mM EDTA0.02% bromophenol blue |
| Application Details: |
Positive control: a 2.5-5 μl load per well is optimal for most chemiluminescent detection systems. |
| Format: |
Liquid |
| Related products: |
AS08 307 | UBQ11 | Ubiquitin antibodies |
| Expected | apparent MW: |
10-12 kDa |
| Not reactive in: |
No confirmed exceptions from predicted reactivity are currently known. |
| Selected references: |
to be added when available |
| Special application note: |
Protein is provided in Laemli SDS loading buffer and should be heated up in 98°C for 5 minutes before loading on the gel. |
|
| U-box domain-containing protein 12 |
Agrisera |
AS13 2728 |
50 µl |
£369.00
|
|
| Background Info: |
U-box domain-containing protein 12 exhibits ligase activity and is involved in protein modifiction. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Storage Temp: |
The antibody may be stored at -20â for one year in its original formulation. Additionally, antibody may be stored at 2â to 8â for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Oryza sativa |
| Immunogen: |
KLH-conjugated synthetic peptide derived from Oryza sativa OsPUB12 protein, UniProt: Q5VRH9 |
| Applications: |
Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
1 : 1000 (WB) |
| Purity: |
Serum |
| Format: |
Lyophilized |
| Reconstitution: |
For reconstitution add 50 µl of sterile water |
| Related products: |
collection of antibodies to proteins involved in signal transductionSecondary antibodies |
| Expected | apparent MW: |
66 | 66 kDa |
| Not reactive in: |
No confirmed exceptions from predicted reactivity are currently known. |
| Selected references: |
to be added when available, antibody released in March 2015. |
| UniProt number: |
Q5VRH9 |
|
| SnRK2.2, SnRK2.3, SnRK2.6 | Ser/Thr-protein kinase SnRK |
Agrisera |
AS14 2783 |
50 µg |
£270.00
|
|
| Background Info: |
SnRK2.2, SnRK2.3, SnRK2.6 are members of SNF1-related protein kinases whose activity is activated by ionic (salt) and non-ionic (mannitol) osmotic stress. Involved in ABA signalling pathway. Alternative names: OST1-kinase-like 2, Protein ATHPROKIN B, SNF1-related kinase 2.3, SnRK2.3, Protein OPEN STOMATA 1SNF1-related kinase 2.6, SnRK2.6, Serine/threonine-protein kinase OST1, OST1-kinase-like 3, Protein ATHPROKIN A, SNF1-related kinase 2.2, SnRK2.2 |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Storage Temp: |
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana |
| Expected Species: |
Brassica oleracea, Glycine max, Medicago sativa, Morsu notabilis, Oryza sativa, Phaseolus vulgaris, Populus sp., Triticum sp., Vicia faba |
| Immunogen: |
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SnRK2.2 UniProt:Q39192 ,TAIR: At3g50500, SnRK2.3, UniProt: Q39193 TAIR: At5g66880, SnRK2.6 UniProt: Q940H6, TAIR: At4g33950 |
| Applications: |
Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
1 : 1000 (WB) |
| Purity: |
Affinity purified serum in PBS, pH 7.4 |
| Format: |
Lyophilized in PBS pH 7.4 |
| Reconstitution: |
For reconstitution add 50 µl of sterile water. |
| Related products: |
AS13 2635 | SRK2E | Ser/Thr-protein kinase SnRK2.6 AS12 1855 | anti-S6K1/2 | Ribosomal S6 kinase 1/2, rabbit antibodiesAS13 2664 | anti-S6K1-2 | Ribosomal-protein S6 kinase homolog 1,2 - phosphorylated, rabbit antibodiesPlant protein extraction buffer Secondary antibodies |
| Expected | apparent MW: |
41 kDa |
| Not reactive in: |
Chlamydomonas reinhardtii |
| Selected references: |
to be added when available, antibody released in September 2015 |
| UniProt number: |
Q940H6 , Q39193 , Q39192 |
| TAIR number: |
At3g50500,At4g33950,At5g66880 |
|
| Mouse polyclonal antibody to human Protocadherin alpha-2: whole serum |
Biosensis |
Mo-950-50 |
50 ul |
£270.00
|
|
| Product Detail: |
Protocadherin alpha-2 is 1 of 15 tandemly arranged genes within the PCDHA gene cluster. These 15 N-terminal 'variable' exons are individually spliced to a downstream C-terminal constant region to form distinct PCDHA transcripts. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain.The PCDH proteins are cadherin-like cell surface proteins that are expressed in neurons and are present at synaptic junctions. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Liquid. 50% glycerol |
| Storage Temp: |
Keep aliquots at -20 °C for higher stability or at 4 °C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. |
| Host Animal: |
Mouse |
| Species Reactivity: |
Human. Other species have not been tested. |
| Immunogen: |
Partial recombinant human PCDH-alpha-2 (189-282) with a GST tag. |
| Applications: |
WB;ELISA |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
Whole serum |
| Antibody Isotype: |
Mixed |
| Application Details: |
This antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Alternative Names: |
PCDH-alpha-2; PCDHA2 |
| Accession Number: |
PCDA2_HUMAN |
| Shelf Life: |
12 months after purchase |
| Specificity: |
Specificity has been confirmed by WB and direct ELISA against the antigen. | Human. Other species have not been tested. |
|
