| Product - please click for more details |
Brand |
Cat No |
Size |
Price (£) |
|
| Mouse anti Beta-Galactosidase from E. coli,clone BG-02 |
Monosan |
MON5013 |
100 ug |
£261.00
|
|
| Storage Temp: |
2-8°C |
| MONOSAN Brand Name: |
MONOSAN |
|
| MaxSignal Tetrahydrocannabinol (THC) ELISA Test Kit |
Bioo Scientific |
5013 |
1 x 96 wells |
£289.00
|
|
| Product Detail: |
Bioo Scientific offers test kits for the detection of regulated substances. Our Scientists have over 30 years of experience developing ELISA test kits. This allows us to produce high quality, dependable reagents with excellent reproducibility and sensitivity. Our expanding line of drug detection assays currently includes over 10 ELISA kits that can be used to screen over 20 drugs and/or their metabolites in a wide range of sample matrices. |
| Background Info: |
Bioo Scientific's enzyme-linked immunosorbent assay (ELISA) test kits are qualitative one-step kits designed as screening devices for the detection of drugs and/or their metabolites in forensic samples. |
| Product Type: |
Forensic Drug Detection |
| Additional Info: |
Intended for forensic toxicology samples only. It is recommended that all suspect samples be confirmed by a quantitative method such as GC/MS. |
| Application Details: |
Please use our contact form above to request a copy of the datasheet and pricing |
|
| AHA1 Antibody |
StressMarq |
SMC-172C |
25 µg |
£126.00
|
|
| Product Detail: |
AHA1 Antibody, Clone 25F2.D9 |
| Background Info: |
Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4). |
| Product Type: |
Antibodies |
| Format: |
0.02M potassium phosphate, 0.15M sodium chloride, pH7.2 |
| Storage Temp: |
-20ºC |
| Host Animal: |
Rat |
| Species Reactivity: |
Hu | Ms | Rt |
| Immunogen: |
Mouse Aha1 |
| Applications: |
WB | IHC | ICC/IF | IP | ELISA |
| Clone number: |
25F2.D9 |
| Antibody Isotype: |
IgG2a Kappa |
| Application Details: |
WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000); optimal dilutions for assays should be determined by the user. |
| Target: |
AHA1 |
| Conjugate: |
Unconjugated |
| Research Area(s): |
Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins |
| Alternative Name(s): |
Aha 1 Antibody, Ahsa1 Antibody, p38 Antibody, HSPC322 Antibody, C14orf3 Antibody, Activator of HSP90 ATPase Antibody, Activator of 90 kDa heat shock protein ATPase homolog 1 Antibody |
| Category: |
Monoclonal |
| Immunogen Species: |
Mouse |
| Accession Number: |
NP_666148.1 |
| Gene ID: |
217737 |
| Swiss-Prot: |
Q8BK64 |
| Species Reactivity Full Name: |
Human | Mouse | Rat |
| Purification: |
Protein G Purified |
| Concentration: |
1.2 mg/ml |
| Specificity: |
Detects ~38kDa. Can run up to 45kDa on SDS Page. |
| Shipping Temperature: |
Blue Ice or 4ºC |
| Certificate of Analysis: |
1 µg/ml of SMC-172 was sufficient for detection of Aha1 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody. |
| Cellular Localization: |
Cytoplasm | Endoplasmic Reticulum |
| References: |
1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub.
2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796.
3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723.
4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
|
| Launch Date: |
1-Jan-2010 |
| Tarriff Code: |
3002.10.0290 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| Cite this Product: |
StressMarq Biosciences Cat# SMC-172C, RRID: AB_2242422 |
|
| NALCN Antibody: PerCP |
StressMarq |
SMC-417D-PCP |
100 µg |
£309.00
|
|
| Product Detail: |
NALCN Antibody, Clone S187-7: PerCP |
| Background Info: |
NALCN (sodium leak channel non-selective protein), alsoknown as CanIon or VGCNL1 (voltage gated channel-like protein 1), is a 1738 amino acid multi-pass membrane protein that belongs to the cation-nonselective channel family. NALCN is highly conserved in mammals and is widely expressed in the central nervous system. Activated by NK-1R, NALCN is a voltage-independent, nonselective cation channel which is permeable to sodium, potassium and calcium ions. NALCN is responsible for background sodium ion leak conductance in neurons and regulates basal excitability of the nervous systems. Defects of NALCN in mice causes disruption in respiratory rhythm and death occurs within 24 hours of birth. Three isoforms of NALCN exists due to alternative splicing events.
|
| Product Type: |
Antibodies |
| Format: |
PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Storage Temp: |
-20ºC |
| Host Animal: |
Mouse |
| Species Reactivity: |
Hu | Ms | Rt |
| Immunogen: |
Fusion protein amino acids 1659-1738 (cytoplasmic C-terminus) of rat NALCN
|
| Applications: |
WB | ICC/IF |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Clone number: |
S187-7 |
| Antibody Isotype: |
IgG1 |
| Application Details: |
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
| Target: |
NALCN |
| Conjugate: |
PerCP |
| Research Area(s): |
Neuroscience | Ion Channels | Sodium Channels | Sodium Leak Channels | Potassium Channels | Calcium Channels |
| Alternative Name(s): |
VGCNL1 Antibody, CanIon Antibody, Sodium leak channel non-selective protein Antibody, Voltage gated channel-like protein 1 Antibody, A530023G15Rik Antibody, bA430M15.1 Antibody, FLJ23913 Antibody, FLJ44659 Antibody, FLJ44764 Antibody, Four repeat voltage gated ion channel Antibody, MGC74524 Antibody, Putative 4 repeat voltage gated ion channel Antibody, Sodium leak channel non-selective protein Antibody |
| Category: |
Monoclonal |
| Immunogen Species: |
Rat |
| Accession Number: |
NP_705894.1 |
| Gene ID: |
266760 |
| Swiss-Prot: |
Q6Q760 |
| Species Reactivity Full Name: |
Human | Mouse | Rat |
| Purification: |
Protein G Purified |
| Concentration: |
1 mg/ml |
| Specificity: |
Detects ~200kDa. |
| Shipping Temperature: |
Blue Ice or 4ºC |
| Certificate of Analysis: |
1 µg/ml of SMC-417 was sufficient for detection of NALCN in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Cellular Localization: |
Membrane |
| References: |
1. Lee, J.H., Cribbs, L.L. and Perez-Reyes, E. (1999)FEBS Lett. 445: 231-236.
2. Lu, B., Su, Y., Das, S., Liu, J., Xia, J. and Ren, D. (2007) Cell 129: 371-383.
3. Jospin, M., et al. (2007) Curr. Biol. 17: 1595-1600.
4. Gaultier, C. and Gallego, J. (2008) Appl. Physiol. 104: 1522-1530.
5. Yeh, E., et al. (2008) PLoS Biol. 6: e55. |
| Launch Date: |
27-Feb-2013 |
| Tarriff Code: |
3002.10.0290 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| Cite this Product: |
StressMarq Biosciences Cat# SMC-417D-PCP, RRID: AB_2701268 |
|
| ERK2 Antibody : PerCP |
StressMarq |
SPC-1144D-PCP |
100 µl |
£310.00
|
|
| Product Detail: |
Rabbit Anti-Human ERK2 Polyclonal |
| Background Info: |
ERK2 or MAPK1 is a protein-serine/threonine kinase. Phosphorylates many diffrent transcription factors, such as ELK1. Acts as transcriptional repressor by binding directly to DNA. Essential for cyclin D1 induction. MAPK1 phosphoryltaes BCL2, which contributes to cell survival, the suppression of the apoptotic effect of BAD and up-regulation of the antiapoptotic protein MCL-1. Regulates accumulation of p53 during DNA damage response. Constitutively active in many human tumours, supposedly due to altered RAS, RAF, EGFR or other upstream elements. ERK/MAPK pathway was shown to promote cell motilty and tumour cell migration. |
| Product Type: |
Antibodies |
| Format: |
PBS pH7.4, 50% glycerol, 0.025% Thimerosal |
| Storage Temp: |
-20ºC |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Hu | Ms |
| Immunogen: |
Synthetic peptide of human ERK2 (AA320-334) |
| Applications: |
WB | AM |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
WB (1:250); optimal dilutions for assays should be determined by the user. |
| Target: |
ERK2 |
| Conjugate: |
PerCP |
| Research Area(s): |
Stem Cells | Signaling Pathways | TGF Beta | Neuroscience | Cell Signaling | Protein Phosphorylation | Serine / Threonine Kinases | MAPK Pathway |
| Alternative Name(s): |
ERK Antibody, ERT1 Antibody, ERK2 Antibody, Extracellular Signal Regulated Kinase 2 Antibody, MAP kinase 1 Antibody, MAP kinase 2 Antibody, MAP kinase isoform p42 Antibody, MAPK1 Antibody, MAPK2 Antibody, Mitogen-activated protein kinase 1 Antibody, Mitogen-activated protein kinase 2 Antibody, MK01_HUMAN Antibody, P38 Antibody, P40 Antibody, P41 Antibody, P42MAPK Antibody, PRKM1 Antibody, PRKM2 Antibody protein kinase, protein tyrosine kinase ERK2 Antibody
|
| Category: |
Polyclonal |
| Immunogen Species: |
Human |
| Accession Number: |
NP_620407 |
| Gene ID: |
5594 |
| Swiss-Prot: |
P28482 |
| Species Reactivity Full Name: |
Human | Mouse |
| Purification: |
Peptide Affinity Purified |
| Specificity: |
Detects 42 kDa. |
| Shipping Temperature: |
Blue Ice or 4ºC |
| Certificate of Analysis: |
A 1:250 dilution of SPC-1144 was sufficient for detection of ERK2 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
| Cellular Localization: |
Cytoplasm | Cytoskeleton | Spindle | Nucleus |
| Launch Date: |
29/Aug/2016 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| Cite this Product: |
StressMarq Biosciences Cat# SPC-1144D-PCP, RRID: AB_2711006 |
|
| Resveratrol |
StressMarq |
SIH-264-100MG |
100 mg |
£48.00
|
|
| Product Detail: |
Antioxidant |
| Background Info: |
Resveratrol is a stilbenoid and a phytoalexin produced naturally by several plants. Its effects are numerous and include anti-cancer (1), anti-inflammatory (2), anti-fungal and has other beneficial cardiovascular effects.
It has been proposed that Mn SOD is increased by the pathwayRESV-SIRT1/Nad+- FOXO3a- MnSOD (3). In terms of its anti-cancer properties, resveratrol modulates the transcription factor NF-KB and inhibition of the cytochrome P450 enzyme (1, 4). |
| Product Type: |
Small Molecules |
| Storage Temp: |
-20ºC |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Research Area(s): |
Cancer | Oxidative Stress |
| Alternative Name(s): |
5-[(E)-2-(4-Hydroxyphenyl)vinyl]-1,3-benzenediol |
| Category: |
Antioxidant |
| Shipping Temperature: |
Shipped Ambient |
| References: |
1. Leiro J., et al. (2005) Int Immunopharm. 5(2): 393-406.
2. Gentilli M., et al. (2001) Life Sciences. 68(11): 1317-1321.
3. Robb E.L., et al. (2008) Biochem Biophys Res Commun. 367(2): 406-412.
4. Chun Y.J., Kim M.Y., Guengerich F.P. (1999) Biochem Biophys Res Commun. 262(1): 20-24. |
| Launch Date: |
8-Apr-2011 |
| Tarriff Code: |
3822.00.1090 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| CAS No.: |
501-36-0 |
| MolecularFormula: |
C14H12O3 |
| Molecular Weight: |
312.3 |
| Source: |
Synthetic |
| Purity: |
>98% (HPLC); NMR (Conforms) |
| Solubility: |
Soluble to 100 mM in DMSO and to 100 mM in ethanol |
| Appearance: |
Off-White Solid |
| Safety Phrases: |
Classification: Caution: Substance not yet fully tested.
Safety Phrases:
S22 - Do not breathe dust
S24/25 - Avoid contact with skin and eyes
S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection
Hazard Phrases:
H302-H315-H317-H318-H335
Precautionary Phrases:
P261-P280-P305 + P351 + P338 |
| Cite this Product: |
Resveratrol (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-264-100MG) |
| PubChem CID: |
445154 |
|
| Resveratrol |
StressMarq |
SIH-264-500MG |
500 mg |
£199.00
|
|
| Product Detail: |
Antioxidant |
| Background Info: |
Resveratrol is a stilbenoid and a phytoalexin produced naturally by several plants. Its effects are numerous and include anti-cancer (1), anti-inflammatory (2), anti-fungal and has other beneficial cardiovascular effects.
It has been proposed that Mn SOD is increased by the pathwayRESV-SIRT1/Nad+- FOXO3a- MnSOD (3). In terms of its anti-cancer properties, resveratrol modulates the transcription factor NF-KB and inhibition of the cytochrome P450 enzyme (1, 4). |
| Product Type: |
Small Molecules |
| Storage Temp: |
-20ºC |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Research Area(s): |
Cancer | Oxidative Stress |
| Alternative Name(s): |
5-[(E)-2-(4-Hydroxyphenyl)vinyl]-1,3-benzenediol |
| Category: |
Antioxidant |
| Shipping Temperature: |
Shipped Ambient |
| References: |
1. Leiro J., et al. (2005) Int Immunopharm. 5(2): 393-406.
2. Gentilli M., et al. (2001) Life Sciences. 68(11): 1317-1321.
3. Robb E.L., et al. (2008) Biochem Biophys Res Commun. 367(2): 406-412.
4. Chun Y.J., Kim M.Y., Guengerich F.P. (1999) Biochem Biophys Res Commun. 262(1): 20-24. |
| Launch Date: |
8-Apr-2011 |
| Tarriff Code: |
3822.00.1090 |
| ADR Code: |
Non-hazardous |
| UN Code for transport: |
Non-hazardous |
| Country of Origin: |
Canada |
| CAS No.: |
501-36-0 |
| MolecularFormula: |
C14H12O3 |
| Molecular Weight: |
312.3 |
| Source: |
Synthetic |
| Purity: |
>98% (HPLC); NMR (Conforms) |
| Solubility: |
Soluble to 100 mM in DMSO and to 100 mM in ethanol |
| Appearance: |
Off-White Solid |
| Safety Phrases: |
Classification: Caution: Substance not yet fully tested.
Safety Phrases:
S22 - Do not breathe dust
S24/25 - Avoid contact with skin and eyes
S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection
Hazard Phrases:
H302-H315-H317-H318-H335
Precautionary Phrases:
P261-P280-P305 + P351 + P338 |
| Cite this Product: |
Resveratrol (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-264-500MG) |
| PubChem CID: |
445154 |
|
| PsbA | D1 protein of PSII, C-terminal (rabbit) |
Agrisera |
AS05 084 |
50 µl |
£270.00
|
|
| Background Info: |
The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1 |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Storage Temp: |
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Anabaena 7120, Arabidopsis thaliana, Artemisia annua, Arundo sp., Chlamydomonas reinhardtii, Chlorella ohadii, Chromera velia, Colobanthus quitensis Kunt Bartl, Coscinodiscus wailesii, Craterostigma sp., Cyanidioschyzon merolae, Cytisus cantabricus (Wilk.) Rchb. F, Ditylum brightwellii, Eucalyptus globulus, Glycine max, Hieracium pilosella L., Hordeum vulgare, Lasallia hispanica, Lindernia sp., Marchantia polymorpha (liverwort), Miscanthus x giganteus, Microcystis aeruginosa, Nicotiana benthamiana, Panicum miliaceum, Panax ginseng, Panicum maximum, Paulinella chromatophora (amoeba), Pheodactylum tricornutum CCAP 1055/1, Physcomitrella patens, Pinus strobus, Prochlorococcus sp. (surface and deep water ecotype), Spartina alterniflora, Spirodela polyrhiza, Symbiodinium sp, Synechococcus sp. PCC 7942, Syntrichia muralis, Triticum aestivum, Zea mays |
| Expected Species: |
Algae (brown and red), Conifers, Cyanobacteria, Dictos, Lycopersicum esculentum, Medicago sativa, Pisum sativum, Sesamum indicum, cellular [compartment marker] of thylakoid membrane |
| Immunogen: |
KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others |
| Applications: |
Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions. In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.Rabbit anti-PsbA antibody can detect more than one band of PsbA protein, e.g. precursor and mature protein as compare to the hen anti-PsbA antibodies AS01 016.This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel.The antibody will bind to cross-linked proteins: D1/D2, D1/cyt b559, D1/CP43. |
| Application Details: |
1 : 10 000 (WB) |
| Purity: |
Serum |
| Format: |
Lyophilized |
| Reconstitution: |
For reconstitution add 50 µl of sterile water. |
| Related products: |
AS01 016S | PsbA protein standard for a quantitative western blotAS05 084PRE | PsbA | D1 protein of PSII, C-terminal , pre-immune serumAS11 1786 | anti-PsbA N-terminal, rabbit antibodyAS10 704 | PsbA | D1 protein of PSII, DE-loop, rabbit antibodyAS13 2669 | PsbA | D1 protein of PSII, phosphorylated, rabbit antibodyrecommended secondary antibodyPlant and algal protein extraction buffer Secondary antibodies |
| Expected | apparent MW: |
38 | 28-30 kDa |
| Not reactive in: |
No confirmed exceptions from predicted reactivity are currently known. |
| Selected references: |
Ananyev et al. (2017). Photosystem II-Cyclic Electron Flow Powers Exceptional Photoprotection and Record Growth in the Microalga Chlorella ohadii.Biochim Biophys Acta. 2017 Jul 19. pii: S0005-2728(17)30105-6. doi: 10.1016/j.bbabio.2017.07.001. Sharwood et al. (2017). Linking photosynthesis and leaf N allocation under future elevated CO2 and climate warming in Eucalyptus globulus. J Exp Bot. 2017 Feb 1;68(5):1157-1167. doi: 10.1093/jxb/erw484. MÃguez et al. (2017). Diversity of winter photoinhibitory responses: A case study in co-occurring lichens, mosses, herbs and woody plants from subalpine environments. Physiol Plant. 2017 Feb 14. doi: 10.1111/ppl.12551. Romanowska et al. (2017). Differences in photosynthetic responses of NADP-ME type C4 species to high light. Planta. 2017 Mar;245(3):641-657. doi: 10.1007/s00425-016-2632-1. Epub 2016 Dec 18. Yang-Er Chen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45â55. Jallet et al. (2016). Photosynthetic physiology and biomass partitioning in the model diatom Phaeodactylum tricornutum grown in a sinusoidal light regime. Algal research, doi:10.1016/j.algal.2016.05.014. Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113. Treves et al. (2016). The mechanisms whereby the green alga Chlorella ohadii, isolated from desert soil crust, exhibits unparalleled photodamage resistance. New Phytol. 2016 Feb 8. doi: 10.1111/nph.13870 Wang et al. (2015). The combined effects of UV-C radiation and H2O2 on Microcystis aeruginosa, a bloom-forming cyanobacterium. Chemosphere. 2015 Jun 16;141:34-43. doi: 10.1016/j.chemosphere.2015.06.020. Bancel et al. (2015). Proteomic Approach to Identify Nuclear Proteins in Wheat Grain. J Proteome Res. 2015 Sep 8. Vandenhecke et al. (2015). Changes in the Rubisco to photosystem ratio dominates photoacclimation across phytoplankton taxa. Photosynth Res. 2015 Jun;124(3):275-91. doi: 10.1007/s11120-015-0137-6. Epub 2015 Apr 11. Charuvi et al. (2015). Photoprotection Conferred by Changes in Photosynthetic Protein Levels and Organization during Dehydration of a Homoiochlorophyllous Resurrection Plant. Plant Physiol. 2015 Apr;167(4):1554-65. doi: 10.1104/pp.114.255794. Spence et al. (2014). Transcriptional responses indicate maintenance of photosynthetic proteins as key to the exceptional chilling tolerance of C4 photosynthesis in Miscanthus à giganteus. J Exp Bot. 2014 Jul;65(13):3737-47. doi: 10.1093/jxb/eru209. Epub 2014 Jun 22. Pandey and Pandey-Rai (2014). Modulations of physiological responses and possible involvement of defense-related secondary metabolites in acclimation of Artemisia annua L. against short-term UV-B radiation. Planta. 2014 Jul 15. Vinyard et al. (2014). Engineered Photosystem II reaction centers optimize photochemistry vs. photoprotection at different solar intensities. J Am Chem Soc. 2014 Mar 3. Malnoë et al. (2014). Thylakoid FtsH Protease Contributes to Photosystem II and Cytochrome b6f Remodeling in Chlamydomonas reinhardtii under Stress Conditions. Plant Cell, Jan 21. |
| Special application note: |
Due to biology of PsbA (D1) protein a number of degradation products can apprear in a sample and may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and 16kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands.Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence. |
| UniProt number: |
P83755 , P0C434 , Q14FH6 , Q6YXN7 , P07753 , P14660 |
| TAIR number: |
ATCG00020 |
|
| cAPX | Ascorbate peroxidase (cytosolic) |
Agrisera |
AS06 180 |
200 µl |
£351.00
|
|
| Background Info: |
Ascorbate peroxidase (APX) is the enzyme catalyzing the ascorbate-dependent reduction of hydrogen peroxide. Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in fruits and photosynthetic tissues. AA is found in every compartment of the plant cell including the apoplast. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Storage Temp: |
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana (Immunohistochemistry only) Dunaliella salina, A. toxicaria, Hordeum vulgare, Pisum sativum, Silene vulgaris, Solanum lycopersicum, Solanum lycopersicum, Solanum tuberosum, Picea silvestris, Zea mays |
| Expected Species: |
Brassica. juncea, Citrus sinensis, Fragaria ananassa, Gossypium hirsutum, Nicotiana tabacum, Solanum tuberosum, Sorghum bicolor, Pinus pinaster, Vitis vinifera |
| Immunogen: |
KLH-conjugated peptide derived from N-terminal of Zea mays cytosolic APX Q41772 |
| Applications: |
Immunoprecipitation (IP), Immunolocalization (IL), Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
2 µg (IP), 1 : 500 (IL), 1 : 10 000 (WB) |
| Purity: |
Total IgG |
| Format: |
Lyophilized in PBS pH 7.4 |
| Reconstitution: |
For reconstitution add 200 µl of sterile water. |
| Related products: |
AS06 184 | anti-AO | apoplastic ascorbate oxidaseAS09 384 | anti-AO | ascorbate oxidaseAS09 383 | anti-AO | ascorbate oxidase, biotinylated antibodyAS08 368 | anti-APX | ascorbate peroxidasePlant protein extraction buffer Secondary antibodies |
| Expected | apparent MW: |
28 kDa |
| Not reactive in: |
Glycine max |
| Selected references: |
Aroca et al. (2015). S-sulfhydration: a new post-translational modification in plant systems. Plant Physiology March 2015 pp.00009.2015. Terrile et al. (2014). Nitric oxide-mediated cell death is triggered by chitosan in F. eumartii spores. Pest Manag Sci. 2014 Apr 25. doi: 10.1002/ps.3814. Tsaniklidis et al. (2013). L-Ascorbic acid metabolism in parthenocarpic and seeded cherry tomatoes. Plant Growth Regul,DOI 10.1007/s10725-013-9845-0. (Solanum lycopersicum, immunolocalization) |
| Special application note: |
Total IgG concentration is 3 µg/µl. |
| UniProt number: |
Q41772 |
| TAIR number: |
ATCG00350 |
|
