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Storage Temp:	2-8°C
MONOSAN Brand Name:	MONOSAN

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Product Detail:	Rabbit anti-VEGF 165
Product Type:	Primary Antibodies
Format:	Lyophilized
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Keywords:	Human VEGF 165
Reactivity:	VEGF 165 (Human)
Conjugate:	Unconjugated
Purification:	Protein A
Host:	Rabbit
Immunogen:	Human VEGF 165
Buffer:	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with no stabilizers or preservatives
Reconstitution:	Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:	Store at 2-8°C after restoration with 0.1mL deionized water or -20°C for long term storage, avoid repeat freeze thaws.
Shelf Life:	5 years
Specificity:	Human VEGF 165
Country Of Origin:	US Origin
Disclaimer:	For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.

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Product Detail:	The NEXTflex Phenylketonuria Amplicon Panel allows you to quickly target and sequence 14 exons of the PAH locus. This kit contains primer pairs and reagents needed to amplify these coding regions of PAH from DNA isolated from fresh or frozen material and prepare libraries for Illumina sequencing. While up to 384 barcodes are currently supplied with the NEXTflex Phenylketonuria Amplicon Panel, the panel has the capability of multiplexing up to 2,000 samples at 100x coverage for detection of germline mutations on a single Illumina 2x300 MiSeq lane. [BENEFITS] 100% coverage of PAH exons and flanking intron-exon boundaries (2.7 kb total) 100% uniformity of amplicons on 0.2x coverage 384 unique barcodes available Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes Low input - Only 20 ng DNA isolated from fresh or frozen samples required Manufactured to the international quality standard ISO 13485
Product Type:	NGS - Illumina Amplicon Panels
Applications:	Phenylketonuria Amplicon Panel
Search Terms:	Illumina Phenylketonuria Amplicon Panel
Application Details:	Please use our contact form above to request a copy of the datasheet
Catalogue Number Update:	Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4250-02
NGS Applications:	Phenylketonuria Panel

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Product Detail:	Bioo Scientific's NEXTflex Cell Free DNA-Seq Kit can produce libraries from 1 ng of circulating tumor DNA (ctDNA) or cell free fetal DNA (cffDNA) isolated from cell free fluids, in two hours or less. This low-input library preparation kit delivers high coverage quality and reduced bias for Illumina sequencing applications.
Background Info:	All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:	NGS - DNA - Illumina Compatible
Applications:	Cell Free DNA-Seq
Search Terms:	Cell Free DNA-Seq
Additional Info:	The NEXTflex Cell Free DNA-Seq Kit can be used to prepare single, paired-end and multiplexed DNA libraries for sequencing using Illumina® platforms. The NEXTflex 1-step End-Repair and Adenylation protocol simplifies the library prep workflow and shortens hands-on library construction time. In addition, the availability of up to 192 unique adapter barcodes facilitates high-throughput applications.
Catalogue Number Update:	Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5150-02

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Product Detail:	Glucocorticoid receptor ligand
Background Info:	Dexamethasone is a potent synthetic member of the glucocorticoid class of steroid drugs. It is an anti-inflammatory and immunosuppressant. In cancer patients, it can be give to augment the antiemetic effect of 5-HT3 receptor antagonists, and is also used to counteract the development of edema (1). In a diagnostic setting, it is used to suppress the natural pituitary-adrenal axis (2).
Product Type:	Small Molecules
Storage Temp:	-20ºC
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Research Area(s):	Cancer \| Apoptosis
Alternative Name(s):	(11?,16?)-9-Fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-diene-3,20-dione
Category:	Inducer
Shipping Temperature:	Shipped Ambient
References:	1. Roila F., and Fatigoni S. (2006) Ann Oncol.17 (Suppl2): ii96-ii100. 2. Hasan E.A., Jessop D.S., Power L.L. Monk P.T. and Kirwan J.R. (2009) Int J Endocrinol. 2009: 391284.
Launch Date:	8-Apr-2011
Tarriff Code:	3822.00.1090
ADR Code:	Non-hazardous
UN Code for transport:	Non-hazardous
Country of Origin:	Canada
CAS No.:	50-02-2
MolecularFormula:	C22H29FO5
Molecular Weight:	278.3
Source:	Synthetic
Purity:	>98% (TLC); NMR (Conforms)
Solubility:	Soluble to 100 mM in DMSO
Appearance:	White solid
Safety Phrases:	Classification: Harmful. May be harmful if inhaled, swallowed or absorbed through skin. Safety Phrases: S22 - Do not breathe dust S24/25 - Avoid contact with skin and eyes S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection Risk Phrases: R62 - Possible risk of impaired fertility R68- Possible risk of irreversible effects Hazard Phrases: H315-H317-H319-H334-H335 Precautionary Phrases: P261-P280-P305 + P351 + P338-P342 + P311
Cite this Product:	Dexamethasone (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-259-1G)
PubChem CID:	5743

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Product Detail:	Glucocorticoid receptor ligand
Background Info:	Dexamethasone is a potent synthetic member of the glucocorticoid class of steroid drugs. It is an anti-inflammatory and immunosuppressant. In cancer patients, it can be give to augment the antiemetic effect of 5-HT3 receptor antagonists, and is also used to counteract the development of edema (1). In a diagnostic setting, it is used to suppress the natural pituitary-adrenal axis (2).
Product Type:	Small Molecules
Storage Temp:	-20ºC
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Research Area(s):	Cancer \| Apoptosis
Alternative Name(s):	(11?,16?)-9-Fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-diene-3,20-dione
Category:	Inducer
Shipping Temperature:	Shipped Ambient
References:	1. Roila F., and Fatigoni S. (2006) Ann Oncol.17 (Suppl2): ii96-ii100. 2. Hasan E.A., Jessop D.S., Power L.L. Monk P.T. and Kirwan J.R. (2009) Int J Endocrinol. 2009: 391284.
Launch Date:	8-Apr-2011
Tarriff Code:	3822.00.1090
ADR Code:	Non-hazardous
UN Code for transport:	Non-hazardous
Country of Origin:	Canada
CAS No.:	50-02-2
MolecularFormula:	C22H29FO5
Molecular Weight:	278.3
Source:	Synthetic
Purity:	>98% (TLC); NMR (Conforms)
Solubility:	Soluble to 100 mM in DMSO
Appearance:	White solid
Safety Phrases:	Classification: Harmful. May be harmful if inhaled, swallowed or absorbed through skin. Safety Phrases: S22 - Do not breathe dust S24/25 - Avoid contact with skin and eyes S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection Risk Phrases: R62 - Possible risk of impaired fertility R68- Possible risk of irreversible effects Hazard Phrases: H315-H317-H319-H334-H335 Precautionary Phrases: P261-P280-P305 + P351 + P338-P342 + P311
Cite this Product:	Dexamethasone (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-259-5G)
PubChem CID:	5743

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Product Detail:	FGFR3 inhibitor
Background Info:	Dovitinib is a potent growth factor receptor kinase inhibitor with effects against FLT-3, c-KIT, VEGFR, PDGFRß and CSF-1R.
Product Type:	Small Molecules
Storage Temp:	-20ºC
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Research Area(s):	Cell Signaling
Alternative Name(s):	4-??amino-??5-??fluoro-??3-??[6-??(4-??methyl-??1-??piperazinyl)?-??1H-??benzimidazol-??2-??yl]?-?2(1H)?-??quinolinone
Category:	Inhibitor
Shipping Temperature:	Shipped Ambient
References:	1. Konecny G.E., et al. (2013) Mol. Cancer Ther. 12(5): 632642.
Launch Date:	1-Oct-2014
Tarriff Code:	3822.00.1090
ADR Code:	Non-hazardous
UN Code for transport:	Non-hazardous
Country of Origin:	Canada
CAS No.:	405169-16-6
MolecularFormula:	C21H21FN6O
Solubility:	Soluble in DMSO
Appearance:	Crystalline solid
Safety Phrases:	Classification: Skin Corrosion/Irritation, Category 2 Serious Eye Damage/Eye Irritation, Category 2 Target Organ Systemic Toxicity (single exposure), Category 3 Safety Phrases: S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. Hazard statements: H315: Causes skin irritation. H319: Causes serious eye irritation. H335: May cause respiratory irritation. Precautionary statements: P302+352: IF ON SKIN: Wash with plenty of soap and water. P332+313: If skin irritation occurs, get medical advice/attention. P305+351+338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P337+313: If eye irritation persists, get medical advice/attention. P304+340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. P312: Call a {POISON CENTER/doctor/...} if you feel unwell.
Cite this Product:	Dovitinib (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-500-25MG)
PubChem CID:	9886808

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Background Info:	The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.This is a recombinant protein standard, source: Synechocystis PCC 6803.
Product Type:	Positive control/Quantitation standard
Format:	Lyophilized in glycerol
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Applications:	Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	Concentration: after adding 95 µl of sterile milliQ water final concentration of the standard is 0.25 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:	Standard curve: 3 loads are recommended (0.5, 2 and 4Î¼l).For most applications a sample load of 0.2 Î¼g of chlorophyll will give a PsbA signal in this range.Positive control: a 2 Î¼l load per well is optimal for most chemiluminescent detection systems.Non-disulphie dependent dimers and complexes can be also detected using standard western blot methods with more sensitive detection reagents as ECL Advance or West Pico when loading per well more standard than recommended. They have not been included in the standard calibration.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:	For reconstitution add 95 µl of sterile water. Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
Related products:	Collection of other protein standardsAS01 016 \| anti-PsbA global hen antibodyAS05 084 \| anti-PsbA global rabbit antibodyCollection of global antibodiesCollection of antibodies to photosynthetic proteins Plant protein extraction buffer
Expected \| apparent MW:	The standard has an actual MW of 41.5 kDa. The presence of a His6 tag causes it to run ~1.7 kDa higher on the gel than the native protein. Note that in most systems, PsbA migrates with an apparent MW of between 30 and 35 kDa.
Selected references:	Yuan et al. (2018). Combined effects of ocean acidification and warming on physiological response of the diatom Thalassiosira pseudonana to light challenges. Mar Environ Res. 2018 Apr;135:63-69. doi: 10.1016/j.marenvres.2018.01.016. Li et al. (2016). A Hard Day's Night: Diatoms Continue Recycling Photosystem II in the Dark. Front. Mar. Sci., 08 November 2016 \| http://dx.doi.org/10.3389/fmars.2016.00218Vandenhecke et al. (2015). Changes in the Rubisco to photosystem ratio dominates photoacclimation across phytoplankton taxa. Photosynth Res. 2015 Apr 11.Wu et al. (2014). Large centric diatoms allocate more cellular nitrogen to photosynthesis to counter slower RUBISCO turnover rates. Front. Mar. Sci., 09 December 2014 \| doi: 10.3389/fmars.2014.00068.Li et al. (2014). The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytol. 2014 Sep 25. doi: 10.1111/nph.13037.
Special application note:	The PsbA protein standard can be used in combination with global anti-PsbA antibodies to quantitate PsbA from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the PsbA protein.Quantitative western blot: detailed method description, video tutorial

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Background Info:	Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.Source of Rubisco standard: Rubisco protein was purified directly from a plant tissue - spinach.
Product Type:	Positive control/Quantitation standard
Format:	Lyophilized in glycerol
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Applications:	Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.Please, use the 55 kDa size of RbcL for calculations. The pmoles in the standard refer to pmoles of rbcL monomers.
Application Details:	Standard curve: three protein standard loads are recommended.For most applications a sample load of 0.2 Î¼g of chlorophyll/well will give a RbcL signal in this range.Positive control: a 2 Î¼l load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains. Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.This standard is stabilized does not require heating before loading on the gel or addition of any buffer.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:	For reconstitution add 95 µl of sterile water. Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
Related products:	Primary antibodies matching Rubisco standard are: AS03 037 \| anti-RbcL \| Rubisco large subunit, form I and form II (50 µl)AS03 037A \| anti-RbcL \| Rubisco large subunit, form I and form II (50 µg affinity purified)AS03 037-HRP\| anti-RbcL \| Rubisco large subunit, form I and form II (40 µg, HRP-conjugated) AS01 017 \| anti-RbcL \| Rubisco large subunit, form I, chicken antibodyAS15 2994 \| Rubisco ELISA quantitation kit matching secondary antibodycollection of other protein standardscollection of other global antibodiesPlant and algal protein extraction buffer
Expected \| apparent MW:	52.7 kDa
Selected references:	Li et al. (2016). Interactive effects of nitrogen and light on growth rates and RUBISCO content of small and large centric diatoms. Photosynth Res. 2016 Aug 26. [Epub ahead of print]Young et al. (2015). Antarctic phytoplankton down-regulate their carbon-concentrating mechanisms under high CO2 with no change in growth rates. Marine Ecology Progress Series 532:13-28.Vandenhecke et al. (2015). Changes in the Rubisco to photosystem ratio dominates photoacclimation across phytoplankton taxa. Photosynth Res. 2015 Apr 11.Wu et al. (2014). Large centric diatoms allocate more cellular nitrogen to photosynthesis to counter slower RUBISCO turnover rates. Front. Mar. Sci., 09 December 2014 \| doi: 10.3389/fmars.2014.00068. Li et al. (2014). The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytol. 2014 Sep 25. doi: 10.1111/nph.13037.
Special application note:	The RbcL protein standard can be used in a combination with Agrisera global antibiodies (AS01 017 from chicken or AS03 037 from a rabbit) to quantitate RbcL from a wide range of species. Global antibodies are raised against highly conserved amino acid sequence. This standard is also included in following kits: Educational antibody kit - photosynthesis, Photosynthesis Tool Kit - quantitation, Rubico quantitation kit,Quantitative western blot: detailed method description, video tutorial

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Background Info:	Nitrogenase is involved in biological fixation of nitrogen to assimilable ammonia.This product is a recombinant protein standard, source: Nostoc/Anabaena 7120.
Product Type:	Positive control/Quantitation standard
Format:	Lyophilized
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Applications:	Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	Concentration: after adding 225 µl of milliQ water final concentration of this standard is 0.15 pmoles/ul and this reagent is ready to use and load on a gel.Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:	Standard curve: 3 loads are recommended (0.5, 2 and 4 Î¼l).For most applications a sample load of 0.2 Î¼g of chlorophyll will give a NifH signal in this range.Positive control: a 2 Î¼l load per well is optimal for most chemiluminescent detection systems. This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:	For reconstitution add 225 µl of sterile water. Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
Related products:	AS01 021A \| matching antibody: anti-NifH hen global antibodiesCollection of other protein standardsCollection of other global antibodiesCollection of antibodies to proteins involved in nitrogen metabolismAlgal protein extraction buffer
Expected \| apparent MW:	34 kDa (larger than a native protein due to the addition of His-tag)
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known.
Selected references:	Levitan et a. (2010). Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO concentrations. Environ. Microbiol (Epub ahead of print)
Special application note:	The NifH protein standard can be used in combination with global anti-NifH antibodies to quantitate NifH protein from a wide range of cyanobacterial species. Global antibodies are raised against highly conserved 15 amino acid sequence found in NifH proteins.Quantitative western blot: detailed method description, video tutorial

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Background Info:	ATP synthase is the universal enzyme that stnthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.This product is a recombinant protein standard, source: Synechocystis strain PCC 6803.
Product Type:	Positive control/Quantitation standard
Format:	Lyophilized, in glycerol
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Applications:	Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	Concentration: after adding 90 µl of dest. water final concentration of the standard is 0.27 pmol/µl.Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:	Standard curve: 3 loads are recommended (0.5, 2 and 4Î¼l).For most applications a sample load of 0.2Î¼g of chlorophyll will give a AtpB signal in this range.Positive control: load per well: a 2Î¼l load is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:	For reconstitution add 90 µl of milliQ water. Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
Related products:	collection of other protein standardsAS03 030 \| anti-ATP synthase subunit beta global antibody (hen)AS05 085 \| anti-ATP synthase subunit beta global antibody (rabbit)collection of other global antibodiesPlant and algal protein extraction buffer
Expected \| apparent MW:	in most gel systems AtpB migrates around 50-54 kDa
Selected references:	Fraser et al. (2013). Photophysiological and Photosynthetic Complex Changes during Iron Starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942. PLOS ONE.
Special application note:	The AtpB protein standard can be used in combination with global anti-AtpB antibodies to quantitate AtpB from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the AtpB protein.Quantitative western blot: detailed method description, video tutorial

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Background Info:	PsaC is a conserved, chloroplast-encoded, Fe-S binding protein of approximately 10kDa, present in all known Photosystem I complexes. It is located on the stromal side of the thylacoid membranes. PsaC coordinates the FeâS clusters FA and FB through two cysteine-rich domains.This product is a recombinant protein standard, source: Synechocystis PCC 6803.The PsaC protein standard can be used in combination with global anti-PsaC antibodies to quantitate PsaC from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the PsaC protein.Quantitative western blot: detailed method description, video tutorial
Product Type:	Positive control/Quantitation standard
Format:	Lyophilized in glycerol
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Applications:	Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	Protein standard buffer composition: Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.
Application Details:	Positive control: a 2 Î¼L load per well is optimal for most chemiluminescent detection systems. Standard curve: 3 loads are recommended (eg. 0.5, 2 and 4Î¼L). For most applications a sample load of 0.2 Î¼g of chlorophyll will give a PsaC signal in this range. Exact loads can vary with the sensitivity of your system and the abundance of the target protein in your samples. Note: Optimal quantitation is achieved using moderate sample loads/well, generally 1 to 5 ug total protein. A trial experiment may be required i) to bring your sample load within the standard curve range and ii) to obtain a signal that is strong enough to reliably quantify but not so strong as to consume ECL reagents too quickly or saturate your detection system. These goals may achieved by adjusting both sample and standard loads.
Reconstitution:	For reconstitution add 95 µl of sterile water. Note that due to glycerol in buffer, the lyophilized product appears as a dense liquid rather than a powder. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently. Avoid vigorous vortexing, as buffer contains detergent. Upon reconstitution, this standard is ready-to-load and does not require any additions or heating. See additional Handling Instructions below. PsaC standard protein concentration: 0.10 pmol/µl.
Related products:	Collection of other protein standardsAS04 042 \| anti-PsaC \| PSI-C core subunit of photosystem I global rabbit antibodiesCollection of other global antibodiesCollection of antibodies to PSI proteins Plant and algal protein extraction buffer
Expected \| apparent MW:	11.5 kDa (larger than native protein due to the addition of His-tag). In most gels PsaC migrates between 9 and 14 kDa
Selected references:	Li et al. (2016). A Hard Day's Night: Diatoms Continue Recycling Photosystem II in the Dark. Front. Mar. Sci., 08 November 2016 \| http://dx.doi.org/10.3389/fmars.2016.00218Vandenhecke et al. (2015). Changes in the Rubisco to photosystem ratio dominates photoacclimation across phytoplankton taxa. Photosynth Res. 2015 Apr 11.Wu et al. (2014). Large centric diatoms allocate more cellular nitrogen to photosynthesis to counter slower RUBISCO turnover rates. Front. Mar. Sci., 09 December 2014 \| doi: 10.3389/fmars.2014.00068.Li et al. (2014). The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytol. 2014 Sep 25. doi: 10.1111/nph.13037.
Special application note:	Handling Instructions*IMPORTANT: In our experience, viscous liquids are surprisingly stable; insufficient mixing is the most common reason for unsatisfactory results. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.Standard needs to be fully thawed and thoroughly mixed before each use. Proteins tend to stratify with the more dense layer after freezing. We recommend bringing the product to room temperature and either mixing by inverting or flicking tube 5-10 times. Pipetting up and down may also provide sufficient mixing, provided the tip is moved within the tube while taking up and expelling the liquid.

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Background Info:	Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.Source of AOX standard: AOX standard source is Sphingomonas wittichii strain RW1, overexpressed in E.coli bearing an N-terminal his6 tag.
Product Type:	Positive control/Quantitation standard
Format:	Lyophilized
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Applications:	Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	Concentration: 0.1 pmol/µl. After re-constitution with sterile milliQ water, the final concentration of the AOX monomer is.0.1 pmol/µl. While a dimer is present in the lane, only the 27 kDa monomer contributes to the calibration.Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.
Application Details:	Standard curve: 3 loads are recommended (0.5, 2 and 4Î¼l).For most applications a sample load of 0.2 Î¼g of chlorophyll/well will give a RbcL signal in this range.Positive control: a 2 Î¼l load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains. Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:	For reconstitution add 100 µl of milliQ water. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
Related products:	Primary antibodies matching AOX standard are: AS04 054 \| AOX1/2 \| plant alternative oxidase 1 and 2, rabbit antibodycollection of other protein standardscollection of matching global antibodiesPlant and algal protein extraction buffer
Expected \| apparent MW:	27 kDa
Selected references:	to be added when available, standard available in May 2015
Special application note:	The AOX calibrated protein standard can be used in combination with Agrisera global anti-AOX antibiodies (AS04 054) to quantitate AOX from a wide range of species. Global antibodies are raised against highly conserved amino acid sequence. Quantitative western blot: detailed method description, video tutorial

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Background Info:	This kit contains antibodies to the following 5 proteins and 4 protein standards for their quantification. It also includes a matching secondary antibody.Rubisco - universal and absolutely essential carbon fixation enzyme that is conserved across oxygenic photoautotrophs and other groups. The RbcL (Rubisco large subunit) protein is hyperabundant and regulation of RbcL content under different conditions or stress responses sets a limit on the maximum capacity for CO2 uptake in a population or community.Proteins involved in conversion of solar energy into chemical bonds:PsaC protein of multisubunit protein complex - Photosystem I, which harnesses light energy required for photosynthesis to occur.PsbA protein of multisubunit complex - Photosystem II - the ultimate source of almost all biosynthetic reductant in the biosphere. PsbA (D1) protein is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA Antibody shows the functional content of Photosystem II in a wide range of samples.Lhcb1 - harnesses light energy for photosynthesis (Photosystem II)AtpB highly conserved across the beta subunits of known F-type ATP Synthases from chloroplasts, mitochondria and most bacteria. The ATP Synthase complex is essential for the synthesis of ATP from ADP and free phosphate
Product Type:	Antibody
Antibody Type:	Polyclonal
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:	Rabbit. Secondary antibody: Goat.
Immunogen:	KLH-conjugated respective synthetic peptides were used
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	Suggested experimental treatments for student labs: - Low light vs. High lightShift from low or moderate light (50-100 umol photons m-2 s-1) to high light (500-2000 umol photons m-2 s-1, or nearly full sunlight). Note: Depending on the species and the developmental stage this will lead to large changes in protein profiles, usually within a few hours and is reflected in detectable differences in LHC & RbcL content, and also differences in the PsbA/PsaC ratio (PSII:PSI).-PsbA breakdown products will become more prominent, and the pool of mature PsbA usually declines -there may be declines in antenna proteins, and sometimes increases in RbcL, if the stress is not too harsh. For higher crop plants, it is probably the most generally reliable, rapid way to provoke detectable changes in protein content, since the PsbA/D1 pool will usually decline. - Changes in nutritional statusShifting from nutrient replete to low-nutrient conditions will cause gradual declines in LHC & RbcL content. Etiolation vs. greening Characteristic change, usually PSI (PsaC) accumulates before PSII (PsbA) and LHCII can be observed. Developmental stagesNewly emerged, young & old leaves will show different profiles, as will (of course) leaves & roots.Suggested material: Plants (spinach, sunflower, pea), Algae or cyanobacteria.Material preparation and extraction: Tissue samples of the size required for later extraction can be frozen in foil sachets and stored at â80ÂºC. Extraction buffer: LiDS is recommended over SDS for extraction involving cold steps. Protease inhibitor: PEFABLOC. For a teaching lab, this may not be strictly necessary, however if extracts are to be refrozen and reused, degradation becomes an issue and addition of protease inhibitor is advised.Recommended extraction volume: 150 mg of leaf tissue into 500 ul of extraction buffer (see appendix). After the extraction by grinding and/or sonication the mixture is centrifuged (full speed in microfuge for 2 min) to separate solubilized extract from bulk leaf material. The recovery of extract should be about 300 ul, depending on the type of leaves. This gives an extract at a concentration of roughly 100 ug chl/ml (0.1 ug chl/ul). Load per well: ca. 0.5 ug chl (so ca. 5 ul of the extract). So 150 mg of leaf tissue should give a recovered extract of 300 ul @ 0.1 ug chl/ul = 30 ug chl, sufficient for about 60 lane loads of 0.5 ug chl/lane for immunoblotting The loads can be reduced easily down to 0.2 ug chl (for mini gels) or even lower (down to 0.01 ug chl in some cases using ultrasensitive detection methods). Lighter loads are recommended for some abundant proteins detected with strong antibodies (like anti-RbcL). Alternatively, for ease of extraction it can be scaled up to 1 g of tissue in 4 ml of extraction buffer. Loads can be prepared based on: chlorophyll, protein or leaf area.Chlorophyll is suitable and simple loading parameter for determination of the level of the photosynthetic apparatus, which accounts for most leaf protein. Therefore loading by equal chlorophyll can show both qualitative and quantitative changes in a protein profile. For leaves, loads by equal area are recommended, as a leaf is nearly 2-dimensional and photosynthetic capacity per leaf area is a useful ecophysiological parameter.Spectrophotometric chl determinationExtract chl from ca. 100 ul of aqueous extract into 900 ul of 80% acetone saturated with MgCO3. Measure at A663 and A750 (Blank=1 ml 80 % acetone). [Chla](Î¼g.ml-1) = 12.7 (A663-A750)x 1000/xQuantitationIt is recommended to run 3 lanes of quantitated standard to generate a standard curve. It can be helpful to perform trial runs to get the quantitation standards and samples in the same range. Immunoblottting with primary and secondary antibodies and ECL shows linearity of detection over about 1 order of magnitude. Bands are detected over a much wider range, but the pseudo-linearity load/response region is only about 10 fold.Agrisera Western blot protocols and additional information about antibodies
Related products:	Plant and algal protein extraction buffer Secondary antibodies
Special application note:	This kit can be used during teaching labs and contains following antibodies and protein standards:Product information - Primary antibodies: Product number: Product name: Reconstitution: Recommended dilution: AS01 004Anti-Lhcb1 antibodyPlease follow the instructions on the lable on each vial.1:2000 for ECL detection.AS03 037Anti-RbcL global antibody Please follow the instructions on the lable on each vial.1:5000-10 000 for ECL detection.AS10 939Anti-PsaC global antibodyPlease follow the instructions on the lable on each vial.1:1000 for ECL detection.AS05 084Anti-PsbA antibodyPlease follow the instructions on the lable on each vial.1:10 000 for ECL detection.AS05 085-10Anti-AtpB antibodyPlease follow the instructions on the lable on each vial.1:2000-5000 for ECL detection. All primary antibodies in this kit are raised in rabbits.Product information - Protein standards/positive controls:Product number:Product name:Reconstitution:Amount to load on the gel:Size:AS01 016SPsbA protein standard Please follow the instructions on the lable on each vial.2 Î¼l (0.25 pmol/ Î¼l)41.5 kDaAS01 017SRbcL (Rubisco) protein standardPlease follow the instructions on the lable on each vial.2 Î¼l (0.056 pmol/ Î¼l)52.7 kDaAS03 030SAtpB protein standardPlease follow the instructions on the lable on each vial.10 Î¼l (0.27 pmol/Î¼l)53.1 kDaAS04 042SPsaC protein standard Please follow the instructions on the lable on each vial.2 Î¼l (0.15 pmol/Î¼l)11.5 kDa**These proteins are larger than a respective native protein due to the addition of His-tag Product information - Secondary antibodiy:AS09 602- Goat anti-Rabbit IgG (H&L), HRP conjugated, 50 Âµl (2x25 Âµl) Educational information about Quantitative western blot can be found here: detailed method description, video tutorial
UniProt number:	O03042 , P05698 , P0C510 , P00877 , P62090 , P69416 , P0C360 , Q00914 , Q31QV2 , P83755 , P0C434 , Q14FH6 , Q6YXN7 , P07753 , P14660 , Q8VZ87 , P04777 , P04778 , Q9C5R6 , Q39141 , P19366 , P83483 , P06541 , A8IQU3
TAIR number:	ATCG00490, ATCG01060,ATCG00020,AT1G29910, AT1G29920, AT1G29930, AT2G34420, AT2G34430, AT5G08670, ATCG00480

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Background Info:	Rieske Iron-Sulfur Protein (Q9ZR03)is located in chloroplast thylakoid membrane as a component of cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. Alternative names: Rieske iron-sulfur protein, RISP, ISP, plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein, proton gradient regulation protein 1This is a recombinant protein standard, source: Synechocystis PCC 6803.
Product Type:	Protein standard
Format:	Lyophilized
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Applications:	Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	Concentration: after adding 225 µl of milliQ water final concentration of the standard is 0.15 pmol/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:	Standard curve: 3 loads are recommended (0.5, 2 and 4Î¼l).For most applications a sample load of 0.2Î¼g of chlorophyll will give a PsbA signal in this range.Positive control:a 2Î¼l load per well is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:	For reconstitution add 225 µl of milliQ water. Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
Related products:	AS07 230 \| anti-PetC, Rieske iron-sulfur protein of Cyt b6/f complexcollection of antibodies to proteins involved in electron transfercollection of other protein standardsCollection of global antibodiesPlant protein extraction buffer
Expected \| apparent MW:	33 kDa (larger than native protein due to the addition of His-tag). In most gel systems, PetC protein migrates at 23 kDa
Selected references:	Wu et al. (2014). Large centric diatoms allocate more cellular nitrogen to photosynthesis to counter slower RUBISCO turnover rates. Front. Mar. Sci., 09 December 2014 \| doi: 10.3389/fmars.2014.00068.Li et al. (2014). The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytol. 2014 Sep 25. doi: 10.1111/nph.13037.
Special application note:	The PetC protein standard can be used in combination with global anti-PetC antibodies to quantitate PetC from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the PetC protein.Quantitative western blot: detailed method description, video tutorial

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Background Info:	Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.Source of HSP70 standard: recombinant Hsp70 of Arabidopsis thaliana UniProt: Q9LHA8, TAIR: AT3G12580
Product Type:	Positive control/Quantitation standard
Format:	Lyophilized in glycerol
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Applications:	Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:	Standard curve: 3 loads are recommended (eg 0.1, 0.2, 0.3 pmol). Adjust range to fit your samples and your experiment. For most applications a sample load of 0.2 Î¼g of chlorophyll/well will give a HSP70 signal in this range.Positive control: a 2 Î¼l load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains. Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:	For reconstitution add 82 µl of steril water. Please notice that this product contains glycerol and might appear as liquid but is provided lyophilized.Final standard concentration is going to be 0.15 pmol/µl.
Related products:	Primary antibody matching HSP70 standard are: AS08 371 \| anti-HSP70 \| heat shock protein 70 cytoplasmic, rabbit antibodycollection of other protein standardscollection of matching global antibodiesPlant and algal protein extraction buffer
Expected \| apparent MW:	70 kDa
Selected references:	to be added when available, product released in September 2015
Special application note:	The HSP70 protein standard can be used in a combination with Agrisera global antibiodies (AS08 371) to quantitate HSP70 from a wide range of species. Global antibodies are raised against highly conserved amino acid sequence. Quantitative western blot: detailed method description, video tutorial

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Background Info:	Glutamine synthetase (GlnA) is the key enzyme in the incorporation of mineral nitrogen into glutamine.This product is a recombinant GlnA protein standard, source Synechocystis strain PCC 6803.
Product Type:	Positive control/Quantitation standard
Format:	Lyophilized
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Applications:	Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	Concentration: after adding 225 µl of milliQ water final concentration of the standard is 0.20 pmol/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:	Standard curve: 3 loads are recommended (0.5, 2 and 4Î¼l).For most applications a sample load of 0.2Î¼g of chlorophyll will give a GlnA signal in this range.Positive control: a 2Î¼l load per well is optimal for most chemiluminescent detection systems. This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:	For reconstitution add 225 µl of sterile water.
Related products:	collection of other protein standardsAS01 018 \| anti-GlnA \| glutamine synthetase hen antibodycollection of other global antibodiescollection of antibodies to proteins involved in nitrogen metabolismPlant and algal protein extraction bufferSecondary antibodies
Expected \| apparent MW:	in most gel systems GlnA migrates around 53 kDa
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known.
Selected references:	to be added when available
Special application note:	The GlnA protein standard can be used in combination with global anti-GlnA antibodies to quantitate GlnA from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the GlnA protein.Quantitative western blot: detailed method description, video tutorial

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Background Info:	D2 protein (PsbD) forms the reaction core of PSII (Photosystem II) as a heterodimer with the D1 protein (PsbA). PsbD is homologous to the D1 protein, with slightly higher molecular mass of about 39,5 kDa. Accumulation of D2 protein is an important step in the assemply of the PSII reaction centre complex.This product is a recombinant protein standard, source Synechocystis strain PCC 6803.
Product Type:	Positive control/Quantitation standard
Format:	Lyophilized in glycerol
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Applications:	Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	Concentration: after adding 225 µl of milliQ water final concentration of the standard is 0.25 pmoles/ulProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:	Standard curve: 3 loads are recommended (0.5, 2 and 4Î¼l).For most applications a sample load of 0.2 Î¼g of chlorophyll will give a PsbD signal in this range.Positive control: a 2 Î¼l load per well is optimal for most chemiluminescent detection systems. This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:	For reconstitution add 225 µl of sterile water. Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
Related products:	Collection of other protein standardsAS06 146 \| anti-PsbD \| D2 protein of PSII antibodiesCollection of other global antibodies Collection of antibodies to PSII proteins
Expected \| apparent MW:	In most gel systems PsbD migrates around 28-30 kDa
Selected references:	Li et al. (2016). A Hard Day's Night: Diatoms Continue Recycling Photosystem II in the Dark. Front. Mar. Sci., 08 November 2016Li et al. (2014). The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytol. 2014 Sep 25. doi: 10.1111/nph.13037.
Special application note:	The PsbD protein standard can be used in combination with global anti-PsbD antibodies to quantitate PsbD from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the PsbD protein.Quantitative western blot: detailed method description, video tutorial

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Background Info:	Phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) serves as an important control element in the regulation of photosynthetic carbon metabolism in C4 and CAM plants. This is the first enzyme of the pathway, and PEPC enzymes are encoded by a small multigenic family. Several isoforms of PEPC have been characterised in maize, sorghum and sugarcane. These isoforms are involved in several functions such as the initial fixation of atmospheric CO2 (= C4 PEPC) and anaplerotic functions associated with nitrogen assimilation or amino acid biosynthesis (Lepiniec et al. 1994).
Product Type:	Positive control/Quantitation standard
Format:	Lyophilized in glycerol
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Applications:	Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.This standard is ready-to-load and does not require any additions or heating.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:	Standard curve: 3 loads are recommended (0.5, 2 and 4Î¼l).For most applications a sample load of 0.2 Î¼g of chlorophyll/well will give a RbcL signal in this range.Positive control: a 2 Î¼l load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains. Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:	For reconstitution add 90 µl of steril water. Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
Related products:	AS09 458 \| anti-PEPC \| phosphoenolpyruvate carboxylase, rabbit antibodyAS07 241 \| anti-PEPCK \| PEP carboxy kinase
Expected \| apparent MW:	110 \| 105 kDa
Selected references:	to be added when available, product released in October 2014.
Special application note:	The PEPC protein standard can be used in a combination with Agrisera global PEPC antibiody to quantitate PEPC from a wide range of species. Global antibodies are raised against highly conserved amino acid sequence. Quantitative western blot: detailed method description, video tutorial

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Background Info:	This product is a recombinant protein standard, source: Synechocystis strain PCC 6803.
Product Type:	Positive control/Quantitation standard
Format:	Lyophilized
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Applications:	Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	Concentration: after adding 225 µl of milliQ water final concentration of the standard is 0.15 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:	Standard curve: 3 loads are recommended (2.5 and 10 Î¼l).For most applications a sample load of 0.2Î¼g of chlorophyll will give a IsiA signal in this range.Positive control:a 2Î¼l load per well is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:	For reconstitution add 225 µl of milliQ water.
Related products:	AS06 111 \| anti-CP43' \| IsiA homolog of plant CP43 antibodiesCollection of global antibodiesCollection of antibodies to photosynthetic proteins Plant and algal protein extraction buffer
Expected \| apparent MW:	27 kDa (slightly larger than native protein due to His-tag)
Selected references:	Fraser et al. (2013). Photophysiological and Photosynthetic Complex Changes during Iron Starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942.PLoS ONE 8(3): e59861. doi:10.1371/journal.pone.0059861Ryan-Keogh et al. (2012). Iron deficiency in cyanobacteria causes monomerization of photosystem I trimers and reduces the capacity for state transitions and the effective absorption cross section of photosystem I in vivo. J. of Phycology, 1:145-154.
Special application note:	The IsiA protein standard can be used in combination with anti-IsiA antibodies to quantitate IsiA from a range of cyanobacteria. Global antibodies are raised against highly conserved amino acid sequences in theIsiA protein.Quantitative western blot: detailed method description, video tutorial

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Background Info:	Pathogenesis-related protein 1 (PR-1) is partially responsible for acquired pathogen resistance. Induced by INA, salicylic acid and pathogen infection.This product is a recombinant PR-1 protein, trunctated by first 26 amino acids, source: Arabidopsis thaliana,UniProt:P33154, TAIR: At2g14610
Product Type:	Positive control/Quantitation standard
Format:	Lyophilized in glycerol
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Applications:	Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	Concentration: after adding 90 µl of sterile milliQ water final concentration of the standard is 0.10 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:	Standard curve: 3 loads are recommended eg.0.5, 2 and 4Î¼l.For most applications a sample load of 10-20 Î¼g of protein will provide with a signal in this range.Positive control:a 2Î¼l load per well is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:	For reconstitution add 90 µl of sterile water. Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
Related products:	AS10 687 \| anti-PR-1 \| Pathogenesis-related protein 1, rabbit antibodyCollection of antibodies to other proteins involved in a response to pathogen attackPlant protein extraction buffer
Expected \| apparent MW:	16.4 kDa
Selected references:	To be added when available
Special application note:	The PR-1 protein standard can be used in combination with anti-PR-1 antibodies to quantitate PR-1 protein. Quantitative western blot: detailed method description, video tutorial
TAIR number:	At2g14610

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Background Info:	Tic40 is a component of the inner envelope membrane import complex (TIC) of plant chloroplasts. Tic40 has been proposed to function as a co-chaperone in the stromal chaperone complex that facilitates protein translocation across the inner membrane. Tic40 can be used as a cellular [compartment marker] for chloroplast inner envelope membrane.
Product Type:	Antibody
Antibody Type:	Polyclonal
Format:	Lyophilized
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:	Rabbit
Species Reactivity:	Arabidosps thaliana, Catharantus roseus, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Vitis vinifera
Expected Species:	Picea sitchenis, Pisum sativum, Populus trichocarpa, Ricinus communis
Immunogen:	KLH-conjugated synthetic peptide derived from available plant sequences of Tic40 including Arabidopsis thaliana At5g16620
Applications:	Immunofluorescence (IF), Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	This product can be sold containing ProClin if requested
Application Details:	1 : 2500 (WB)
Purity:	Serum
Reconstitution:	For reconstitution please add 50 µl of sterile water.
Related products:	AS10 709-10 \| anti-Tic40, smaller antibody packAS06 150 \| anti-Toc75AS08 293 \| anti-Tic110Plant protein extraction buffer Secondary antibodies \|AgriseraSuperDeal
Expected \| apparent MW:	48 \| 45 kDa (Arabidopsis thaliana)
Not reactive in:	Chlamydomonas reinhardtii
Selected references:	Wu et al. (2018). Control of Retrograde Signaling by Rapid Turnover of GENOMES UNCOUPLED 1. Plant Physiol. 2018 Jan 24. pii: pp.00009.2018. doi: 10.1104/pp.18.00009. Yang et al. (2016). OsCLT1, a CRT-like transporter 1, is required for glutathione homeostasis and arsenic tolerance in rice. New Phytol. 2016 Feb 25. doi: 10.1111/nph.13908 Yang et al. (2016). LIR1 regulates light-dependent attachment of LFNR to the thylakoid membrane in plants. Plant Cell. 2016 Mar 3. pii: tpc.01027.2015. RomÃ¡n et al. (2015). Non-redundant contribution of the plastidial FAD8 Ï-3 desaturase to glycerolipid unsaturation at different temperatures in Arabidopsis. Mol Plant. 2015 Jun 13. pii: S1674-2052(15)00267-1. doi: 10.1016/j.molp.2015.06.004. Yin et al. (2014). The membrane proteome of stroma thylakoids from Arabidopsis thaliana studied by successive in-solution and in-gel digestion. Physiol Plant. 2014 Nov 17. doi: 10.1111/ppl.12308. Wang et al. (2014). Maintenance of Chloroplast Structure and Function by Overexpression of the Rice MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE Gene Leads to Enhanced Salt Tolerance in Tobacco. Plant Physiol. 2014 May 19;165(3):1144-1155. Brillouet et al. (2013).The tannosome is an organelle forming condensed tannins in the chlorophyllous organs of Tracheophyta. Ann Bot. Sep. 11. (Vitis vinifera, immunofluorescence)
Special application note:	Cellular [complartment marker] of chloroplast membrane
UniProt number:	Q9FMD5
TAIR number:	AT5G16620

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Background Info:	Histone 1 (H1) is a protein located in nuclei, incorporated into chromatin.
Product Type:	Antibody
Antibody Type:	Polyclonal
Format:	Lyophilized
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:	Rabbit
Species Reactivity:	Arabidopsis thaliana, Nicotiana tabacum, Triticum aestivum
Expected Species:	Lathyrus sativus, Phaseolus vulgaris, Pisum sativum, Solanum lycopersicum, Vicia faba
Immunogen:	native H1 protein purified from Nicotiana tabaccum (H1A, H1B H1C,D,E,F)
Applications:	Chromatin Immunoprecipitation (IP) (ChIP), Western blot (WB), Immunocytochemistry (ICC) (ICC)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	Protocol for isolation of cytosolic and nuclear fractions can be found here.Protocol for immunostatining in whole-mount plant tissues: An efficient method for quantitative, single-cell analysis of chromatin modification and nuclear architecture in whole-mount ovules in Arabidopsis Wenjing She, Daniel Grimanelli, Célia Baroux Journal of Vizualized Experiments (JoVE), in press
Application Details:	1 : 100-1 : 500 (ICC), 1 : 5000 (WB)
Purity:	Affinity purified serum in PBS, pH 7.4
Reconstitution:	For reconstitution add 50 µl of sterile water.
Related products:	AS10 710 \| H3 \| histone H3, rabbit antibody AS11 1753 \| HDT3 \| histone deacetylase, rabbit antibody collection of antibodies to DNA/RNA/cell cyclePlant protein extraction buffer Secondary antibodies \|AgriseraSuperDeal
Expected \| apparent MW:	15 \| 17 kDa
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known.
Selected references:	Wollmann et al. (2017). The histone H3 variant H3.3 regulates gene body DNA methylation in Arabidopsis thaliana. Genome Biol. 2017 May 18;18(1):94. doi: 10.1186/s13059-017-1221-3. She and Baroux (2015). Chromatin dynamics in Pollen Mother Cells underpin a common scenario at the somatic-to-reproductive fate transition of both the male and female lineages in Arabidopsis. Front. Plant Sci. \| doi: 10.3389/fpls.2015.00294. She et al. (2013). Chromatin reprogramming during the somatic to-reproductive cell fate transition in plants. Development Oct;140(19):4008-19. doi: 10.1242/dev.095034. Epub 2013 Sep 4. (Arabidopsis thaliana, immunostaining)

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Background Info:	LSD1 (Lesion simulating disease 1) is a negative regulator of reactive oxygen-induced cell death, cold stress-induced cell death, pathogen-induced hypersensitive response (HR), basal disease resistance. The protein is required for leaf acclimation in response to excess excitation energy. Alternative names: CHILLING SENSITIVE 4, CHS4.
Product Type:	Antibody
Antibody Type:	Polyclonal
Format:	Lyophilized in PBS pH 7.4
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:	Rabbit
Species Reactivity:	Arabidopsis thaliana
Expected Species:	Brassica olereacea, Pisum sativum
Immunogen:	KLH-conjugated synthetic peptide derived from Arabidopsis thaliana LSD1 sequence, UniProt:P94077, TAIR: AT4G20380
Applications:	Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Application Details:	1 : 1000 (WB)
Purity:	Affinity purified serum in PBS, pH 7.4
Reconstitution:	For reconstitution add 50 µl of sterile water.
Related products:	AS12 1854 \| Anti-NPR1 Nonexpresser of PR genes 1, rabbit antibodies AS12 1867 \| anti-HY5 \| Protein long hypocotyl 5, rabbit antibodiesAS12 2104 \| anti-LSD1 \| Lesion simulating disease 1, chicken antibodies available for free for testingcollection of antibodies to developmental biologyPlant protein extraction buffer Secondary antibodies \|AgriseraSuperDeal
Expected \| apparent MW:	20 kD
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known.
Selected references:	Chai et al. (2015). LSD1 and HY5 antagonistically regulate red light induced-programmed cell death in Arabidopsis. Front Plant Sci. 2015 May 5;6:292. doi: 10.3389/fpls.2015.00292. eCollection 2015.
UniProt number:	P94077
TAIR number:	AT4G20380

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Background Info:	SBPase (Sedoheptulose-1,7-bis phosphatase) is a chloroplast enzyme involved in the carbon reduction of the Calvin cycle, part of carbohydrate metabolism.Source of SBPase standard: SBPase standard source is Sphingomonas wittichii strain RW1, overexpressed in E.coli bearing an N-terminal his6 tag.
Product Type:	Positive control/Quantitation standard
Format:	Lyophilized
Storage Temp:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Applications:	Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:	Standard curve: 3 loads are recommended (0.5, 2 and 4Î¼l).For most applications a sample load of 0.2 Î¼g of chlorophyll/well will give a RbcL signal in this range.Positive control: a 2 Î¼l load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains. Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:	For reconstitution add 85 µl of sterile water. Please note that this product contains glycerol and might appear as liquid but is provided lyophilized.
Related products:	Primary antibodies matching SBPase standard are: AS15 2873 \| anti-SBPase \| Sedoheptulose-1,7-bis phosphatase , rabbit antibodiescollection of other protein standardscollection of matching global antibodiesPlant and algal protein extraction buffer
Expected \| apparent MW:	27 kDa
Selected references:	to be added when available, standard available in November 2015
Special application note:	The SBPase calibrated protein standard can be used in combination with Agrisera global anti-SBPase antibiodies (AS15 2873) to quantitate SBPase from a wide range of species. Global antibodies are raised against highly conserved amino acid sequence. Quantitative western blot: detailed method description, video tutorial