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Product - please click for more details Brand Cat No Size Price (£)
NEXTprep-Mag™ cfDNA Isolation Kit (3-5ml) Bioo Scientific NOVA-3825-03 50 isolation £681.00
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Product Detail: The NextPrep-Mag™ cfDNA Isolation Kit is designed for extracting cell-free DNA (cfDNA) for constructing NGS libraries from human blood plasma or serum, using a fast magnetic bead-based format requiring minimal hands-on time. The isolated cell free DNA is ideal for next generation sequencing and PCR analysis. Suitability of the DNA for use in NGS library construction has been verified using the Bioo Scientific NEXTflex™ Cell Free DNA-Seq Library Prep Kit for Illumina Sequencing and the NEXTflex Cell Free DNA Library Prep Kit for Ion Proton and Ion PGM Sequencing. This kit is optimized for isolating circulating tumor and cell free circulating fetal DNA from blood plasma.

Cell Free DNA Isolation Protocol:
This cell free DNA isolation procedure, which can be completed in approximately 30 minutes, includes an initial lysis and DNA binding step, wash steps, and elution of cfDNA from the magnetic beads. A distinguishing feature of the kit is the very rapid magnetic bead attraction steps.

Cell Free DNA Isolation Challenges:
Extraction of DNA from plasma is challenging because the DNA is highly fragmented and present at very low concentration, especially in healthy individuals. Because the expected yields are low, the DNA recovered cannot be reliably detected using standard methods such as NanoDrop spectrophotometer, gel or capillary electrophoresis, or DNA binding dyes.


NextPrep-Mag cfDNA Isolation Kit Outperforms the QIAamp™ Circulating Nucleic Acid Kit
The NextPrep-Mag cfDNA Isolation Kit offers advantages over the QIAamp™ Circulating Nucleic Acid Kit, including speed of cfDNA isolation (just 30 minutes) and ease of automation (Mag-bead based), while delivering comparable cfDNA yields and higher concentration NGS libraries.
Background Info: All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type: NGS - cfDNA - Isolation
Applications: Cell Free DNA-Seq
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
Search Terms: Cell Free DNA-Seq Kit for Ion, Cell Free DNA-Seq Kit for Illumina
Additional Info: The NextPrep-Mag™ cfDNA Isolation Kit is designed for extracting cell-free DNA (cfDNA) for constructing NGS libraries from human blood plasma or serum, using a fast magnetic bead-based format requiring minimal hands-on time. The isolated cell free DNA is ideal for next generation sequencing and PCR analysis. Suitability of the DNA for use in NGS library construction has been verified using the Bioo Scientific NEXTflex™ Cell Free DNA-Seq Library Prep Kit for Illumina Sequencing and the NEXTflex Cell Free DNA Library Prep Kit for Ion Proton and Ion PGM Sequencing. This kit is optimized for isolating circulating tumor and cell free circulating fetal DNA from blood plasma.

Cell Free DNA Isolation Protocol
This cell free DNA isolation procedure, which can be completed in approximately 30 minutes, includes an initial lysis and DNA binding step, wash steps, and elution of cfDNA from the magnetic beads. A distinguishing feature of the kit is the very rapid magnetic bead attraction steps.

Cell Free DNA Isolation Challenges
Extraction of DNA from plasma is challenging because the DNA is highly fragmented and present at very low concentration, especially in healthy individuals. Because the expected yields are low, the DNA recovered cannot be reliably detected using standard methods such as NanoDrop spectrophotometer, gel or capillary electrophoresis, or DNA binding dyes.

NextPrep-Mag cfDNA Isolation Kit Outperforms the QIAamp™ Circulating Nucleic Acid Kit
The NextPrep-Mag cfDNA Isolation Kit offers a number of advantages over the QIAamp™ Circulating Nucleic Acid Kit, including speed of cfDNA isolation and ease of automation, while delivering comparable cfDNA yields and higher concentration NGS libraries.

DNA libraries constructed with the NEXTflex™ Cell Free DNA-Seq Kit, from cfDNA isolated from plasma of two healthy donors, using either the NextPrep-Mag cfDNA Isolation Kit or the QIAamp Circulating Nucleic Acid Kit. Libraries were amplified for 9 PCR cycles. Input of DNA for each library was 32 ?L, representing 52% of the cfDNA extracted from 5 mL of plasma.

Kit Specs
The NextPrep-Mag cfDNA Isolation Kit For < 1 mL - 3 mL Plasma Samples contains sufficient reagents for extraction of DNA from 50 samples of 1 mL plasma or less, or 16 samples of 3 mL plasma. The NextPrep-Mag cfDNA Isolation Kit For 3 mL - 5 mL Plasma Samples contains sufficient reagents for extraction of DNA from 50 samples of plasma ranging from 3 mL to 5mL. The kit components are stable for at least 12 months from time of receipt, when properly stored.