The MaxDiscovery Cholesterol Enzymatic Assay Kit is a plate-based colorimetric enzymatic assay for the determination of cholesterol in serum samples.
The MaxDiscovery Cholesterol Enzymatic Assay Kit uses a coupled enzymatic reaction scheme: cholesterol esters are first converted to cholesterol and fatty acids. Next, cholesterol is oxidized with O2 to form cholesten-3-one + H2O2. Lastly, the hydrogen peroxide is reacted with 4-aminoantipyrine and p-HBS to yield quinoneimine (red dye) and water. The absorption measured at 520 nm, is proportional to the concentration of cholesterol in the sample. The kit also comes with a control solution containing a cholesterol standard (500 mg/dl) which can be used to calibrate the assay.
BENEFITS:- High sensitivity with low detection limit (25 mg/dl) Robust kinetic assay Rapid results in 10 minutes Does not require expensive instrumentation Highly reproducible results Measure absorbance in visual spectrum (520 nm)
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.