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Product - please click for more details Brand Cat No Size Price (£)
RNaseA 1 mg/mL Bioo Scientific 344005 1 mL £40.00
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Product Detail: RNase A is a highly active ribonuclease purified from bovine pancreas. It cleaves at the 3' side of phosphodiester bonds after pyrimidine nucleotides in single-stranded RNA. RNase A does not cleave DNA since DNA bases do not have the 2'-hydroxyl group required by RNase A for forming the cyclic intermediate cleavage product. The molecular weight of RNase A is 13.7 kDa. RNase A does not require any co-factors for activity.
Background Info: All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type: Molecular Biology Reagents
Additional Info: PRODUCT FORMAT: RNase A is provided as a 1 mg/mL ready-to-use solution in buffer containing 50% glycerol.

ACTIVITY: RNase A is active over a wide range of temperature and pH conditions.

COMMON USES: RNase A is commonly used in DNA extraction protocols, for example to remove bacterial RNA during isolation of plasmid or bacterial DNA. RNase A is also used in ribonuclease protection assays and to remove contaminating RNA from protein preparations.

INACTIVATION: RNase A can be inactivated and removed from reactions by protease digestion followed by phenol/chloroform extraction and alcohol precipitation.

CONDITIONS: Typically 1 – 5 uL of RNase A (1 mg/mL) is added to small-scale DNA purification reactions (carried out in microfuge tube format). The RNase A digestion reaction is incubated for about 10 – 30 min at room temp or 37ºC. The exact time and temperature used are not critical.

STORAGE: Stored at -20ºC

SHELF LIFE: 1 year
Application Details: Please use our contact form above to request a copy of the datasheet