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Product - please click for more details Brand Cat No Size Price (£)
MaxDiscovery Beta-Galactosidase ELISA Kit Bioo Scientific 3420-01 1 x 96 wells £456.00
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Product Detail: The MaxDiscovery Beta-Galactosidase ELISA Kit is an enzyme immunoassay that analyzes the quantity of Beta-galactosidase in cells, tissues or serum. Beta-galactosidase is a hydrolase enzyme that catalyzes the hydrolysis of Beta-galactosides into monosaccharides. The Beta-galactosidase protein is a large (120 kDa, >1000 amino acids) protein that forms a tetramer. In E. coli the Beta-galactosidase protein is encoded by the lacZ gene which is part of the lac operon. The enzyme's function in E. coli is to cleave the disaccharide lactose to form glucose and galactose so they can be used as carbon/energy sources. The lacZ gene from E. coli is one of the most commonly used reporter genes for testing the efficiency of expression vector mediated gene transfer and for studying the regulation of gene promoters. Beta-galactosidase is not typically expressed in mammalian cells, however; Beta-galactosidase positive staining is a marker for mammalian cellular senescence. Beta-galactosidase is a useful reporter gene both in cell culture and in vivo applications due to its ease of detection.
Like most ELISA assays, the MaxDiscovery Beta-Galactosidase ELISA test relies on a Horseradish peroxidase (HRP) conjugated primary antibody and the TMB (3,3',5,5'-tetramethylbenzidine) substrate. TMB is a chromogen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) and has a major absorbance at 370 nm and 652 nm. The color then changes to yellow with the addition of acid with maximum absorbance at 450 nm. The relative amount of Beta-galactosidase protein in the cells will be directly proportional to the amount of signal that is obtained and thus is an excellent technique for detecting protein expression within cell lines.

BENEFITS:-
• Highly sensitive and reproducible
• Fast and simple protocols
• Suited for automation
• Ideal for siRNA studies
Background Info: All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type: Protein Detection - Reporter Assays
Additional Info: FORMAT: 1 x 96 well (Removable 8 wells x 12 strips)

MEASUREMENT: microtiter plate reader (450 nm)

TESTS PER KIT: The kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards and negative controls).

STANDARD LEVELS: 0 / 1 / 2 / 5 / 8 / 10 ug/mL

INCUBATION TIME: 1 hour and 45 minutes

SHELF LIFE: 6 months when stored under optimal conditions

SAMPLE SIZE: 100 uL/well

SAMPLE TYPES: cells, serum and tissue
Cat_no: 342001