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MaxSignal Nitrofurantoin (AHD) ELISA Kit Bioo Scientific 1070-02 1 x 96 wells Call for Pricing
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Product Detail: The MaxSignal Nitrofurantoin (AHD) ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of nitrofurantoin in feed, fish, shrimp, meat, eggs, honey, milk, serum and urine. The new version of this kit allows for the accurate quantitation of AHD at a broader range than any of the other commercially available kits with standards ranging from 0.025 to 6.4 ppb.

To reduce the time and costs associated with sample extraction, Bioo Scientific has developed a single protocol that can be used for testing samples with all of our ELISAs for the detection of nitrofuran antibiotics. Now you can take one sample, perform one extraction and test for AHD, SEM, AMOZ and AOZ.

The MaxSignal Nitrofurantoin (AHD) ELISA Test Kit is based on a competitive colorimetric ELISA assay. The AHD antibody has been coated in the plate wells. During the analysis, sample is added along with the AHD-horseradish peroxidase (AHD-HRP) conjugate. If the AHD residue is present in the sample, it will compete for the AHD antibody; thereby, preventing the AHD-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the AHD residue concentration in the sample.

Nitrofurantoin belongs to the class of 5-nitrofuran antibiotics and has been widely and effectively used for the prevention and treatment of gastrointestinal infections caused by Escherichia coli and Salmonella spp. in cattle, pigs and poultry. It was also used as a growth promoter in food-producing animals. However, both WHO and the European Union (EU) are unable to assign a maximum residue limit for nitrofurantoin because of the potential carcinogenic effects of its residues on human health. As a consequence, the administration of nitrofurantoin to food-producing animals has been prohibited. Because of the rapid excretion of the nitrofurans and their instability in vitro and in vivo, it is impossible to monitor residues of the parent drug nitrofurantoin directly. Alternatively, 1-aminohydantoin (AHD), the major metabolite of nitrofurantoin, which is stable in tissue, even after long-term storage, is selected to monitor.

This kit is manufactured to the international quality standard ISO 9001:2008.
ISO CI#: SARA-2009-CA-0114-01-B

BENEFITS:-
• Offers a rapid and cost-effective extraction methods for various samples offering recovery rates of 75 – 95%
• High sensitivity (0.025 ppb)
• Low detection limit - 0.05 ppb
• Best standard range available - 0.025 - 6.4 ppb
• A quick ELISA assay (less than 1.5 hours regardless of number of samples)
• High reproducibility
Background Info: All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type: Food safety - Drug residues - Antibiotics
Additional Info: MaxSignal Nitrofurantoin (AHD) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.
Application Details: Please use our contact form above to request a copy of the datasheet and pricing
Cat_no: 107002