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Product - please click for more details Brand Cat No Size Price (£)
PsbB | CP47 protein of PSII Agrisera AS04 038 50 µl £357.00
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Background Info: PsbB (CP47) is a chlorophyll-binding protein located in the membrane, where it serves as the core antenna of Photosystem II.
Product Type: Antibody
Antibody Type: Polyclonal
Storage Temp: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal: Rabbit
Species Reactivity: Anabaena 7120, Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Malus prunifolia, Opephoraguenter-grassii (diatom), Oryza sativa, Phaseolus vulgaris, Physcomitrella patens, Pisum sativum, Synechococcus PCC7942, 6803, , Seminavis robusta (diatom)
Expected Species: Abies concolor, Brachypodum distachyon, Brassica napus, Cyanobacteria, Cucumis sativus, Ephedra sp., Glycine max, Lotus japonicus, Nanochloropsis sp. , Nicotiana tabacum, Panax ginseng, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Triticum aestivum, Vitis vinifera
Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbB sequences including Arabidopsis thaliana AtCg00680, Hordeum vulgare P10900, Oryza sativa P0C364, Synechocystis PCC 6803 P05429
Applications: Western blot (WB)
Additional Info: This product can be sold containing ProClin if requestedin bis-tris gel systems PsbB protein migrates between 40-45 kDa
Application Details: 1 : 2000 (WB)
Purity: Serum
Format: Lyophilized
Reconstitution: For reconstitution add 50 µl of sterile water.
Related products: AS04 038S | PsbB protein standard for quantitation and positive control is discontinued. We recommend to use PsbD | D2 together with PsbD antibodyAS04 038PRE | PsbB | CP47 protein of PSII, pre-immune serumantibodies to other PSII proteinsrecommended secondary antibodyPlant and algal protein extraction bufferSecondary antibodies
Expected | apparent MW: 56 kDa
Not reactive in: No confirmed exceptions from predicted reactivity are currently known.
Selected references: Blommaert et al. (2017). Contrasting NPQ dynamics and xanthophyll cycling in a motile and a non-motile intertidal benthic diatom. Limnol. Oceanogr. doi: 10.1002/lno.10511 Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526. Hu et al. (2017). The SUFBC2 D Complex is Required for the Biogenesis of All Major Classes of Plastid Fe-S Proteins. Plant J. 2017 Jan 19. doi: 10.1111/tpj.13483. Fan et al. (2016). Proteome Analyses Using iTRAQ Labeling Reveal Critical Mechanisms in Alternate Bearing Malus prunifolia. J Proteome Res. 2016 Oct 7;15(10):3602-3616. Kowalewska et al. (2016). Three-dimensional visualization of the internal plastid membrane network during runner bean chloroplast biogenesis. Dynamic model of the tubular-lamellar transformation. The Plant Cell March 21, 2016 tpc.01053.2015. Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113 Liu and Last (2015). A land plant-specific thylakoid membrane protein contributes to photosystem II maintenance in Arabidopsis thaliana. Plant J. 2015 Jun;82(5):731-43. doi: 10.1111/tpj.12845. Epub 2015 Apr 29. Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675. Bhuiyan et al. (2015). MET1 Is a Thylakoid-Associated TPR Protein Involved in Photosystem II Supercomplex Formation and Repair in Arabidopsis. Plant Cell. 2015 Jan 13. pii: tpc.114.132787. Sun et al. (2014). Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes. Biochim Biophys Acta. 2014 Nov 22. pii: S0005-2728(14)00650-1. doi: 10.1016/j.bbabio.2014.11.005.
Special application note: This antibody can be used as a loading control for studies of PSIi or photosynthetic acclimation in diatoms Blommaert et al. 2017. Limnol. Oceanogr. DOI: 10.1002/lno.10511.This product can be sold containing proclin if requested.
UniProt number: P56777 , P10900 , P0C364
TAIR number: ATCG00680