| Background Info: |
Pyruvate decarboxylase (PDC) is a homotetrameric enzyme (E.C.4.1.1.1) that catalyses the decarboxylation of pyruvic acid to acetaldehyde carbon dioxide in the cytoplasm. It is also called 2-oxo-acid carboxylase, and pyruvic decarboxylase. In anaerobic conditions, this enzyme is part of the fermentation process that occurs in yeast, especially the Saccharomyces genus, to produce ethanol by fermentation. Pyruvate decarboxylase starts this process by converting pyruvate into acetaldehyde and carbon dioxide. Pyruvate decarboxylase depends on cofactors thiamine pyrophosphate (TPP) and magnesium. This enzyme should not be mistaken for the unrelated enzyme pyruvate dehydrogenase, an oxidoreductase (EC 1.2.4.1), that catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized |
| Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana, Oryza sativa, Zymomonas mobilis |
| Expected Species: |
Aegilops tauschii , Brassica napus, Chlamydomonas reinhardtii, Cocos nucifera, Dichanthelium oligosanthes, Fragaria ananassa, Hordeum vulgare, Glycine max, Nannochloropsis gaditana, Nicotiana tabacum, Panicum miliaceum, Phoenix dactylifera, Pisum sativum, Potamogeton distinctus, Saccharum officinarum, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Rosa chinensis, Trifolium pratense, Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us |
| Immunogen: |
KLH-conjugated peptide derived from available PDC sequences including Arabidopsis thaliana, PDC1 UniProt: O82647, TAIR: AT4G33070 and PDC2 UniProt: Q9FFT4 TAIR: AT5G54960 |
| Applications: |
Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
1 : 10 000 (WB) |
| Purity: |
Serum |
| Reconstitution: |
For reconstitution add 100 µl of sterile water |
| Molecular Weight: |
65 | 65 kDa (Arabidopsis thaliana) |
| Not reactive in: |
No confirmed exceptions from predicted reactivity are currently known |
| Selected references: |
Ventura et al. (2020). Arabidopsis phenotyping reveals the importance of alcohol dehydrogenase and pyruvate decarboxylase for aerobic plant growth. Sci Rep. 2020 Oct 7;10(1):16669. doi: 10.1038/s41598-020-73704-x. PMID: 33028901; PMCID: PMC7542448.Gil-Monreal et al. (2019). ERF-VII transcription factors induce ethanol fermentation in response to amino acid biosynthesis-inhibiting herbicides. J Exp Bot. 2019 Aug 6. pii: erz355. doi: 10.1093/jxb/erz355. Giuntoli et al. (2014). A trihelix DNA binding protein counterbalances hypoxia-responsive transcriptional activation in Arabidopsis. PLoS Biol. 2014 Sep 16;12(9):e1001950. doi: 10.1371/journal.pbio.1001950. eCollection 2014. |
| UniProt number: |
O82647, Q9FFT4 |
| TAIR number: |
AT5G54960, AT4G33070 |
| Research area: |
Arabidopsis thaliana antibodies |
| Code: |
Stress20 |
|
| Background Info: |
Alcohol dehydrogenase (ADH) is an enzyme playing a crucial role in the fermentative metabolism in plants subjected to low oxygen stress. It is known to be synthesized preferentially under low oxygen conditions. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized |
| Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana, Fragaria vesca, Oryza sativa, Phaseolus vulgaris, Solanum lycopersicum |
| Expected Species: |
Species of your interest not listed? Contact us |
| Immunogen: |
KLH-conjugated peptide derived from available ADH sequences including Arabidopsis thaliana P06525, At1g77120 |
| Applications: |
Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
1 : 3000 (WB) |
| Purity: |
Serum |
| Reconstitution: |
For reconstitution add 100 µl of sterile water |
| Molecular Weight: |
42 | 42 kDa (Arabidopsis thaliana) |
| Not reactive in: |
Allyl alcohol dehydrogenase of Nicotiana tabacum, accession 75206691 and in Chlamydomonas reinhardtii. |
| Selected references: |
Czernicka et al. (2022). Proteomic Studies of Roots in Hypoxia-Sensitive and -Tolerant Tomato Accessions Reveal Candidate Proteins Associated with Stress Priming. Cells. 2022 Jan 31;11(3):500. doi: 10.3390/cells11030500. PMID: 35159309; PMCID: PMC8834170.Ventura et al. (2020). Arabidopsis phenotyping reveals the importance of alcohol dehydrogenase and pyruvate decarboxylase for aerobic plant growth. Sci Rep. 2020 Oct 7;10(1):16669. doi: 10.1038/s41598-020-73704-x. PMID: 33028901; PMCID: PMC7542448.Gil-Monreal et al. (2019). ERF-VII transcription factors induce ethanol fermentation in response to amino acid biosynthesis-inhibiting herbicides. J Exp Bot. 2019 Aug 6. pii: erz355. doi: 10.1093/jxb/erz355.Bui et al. (2019). Conservation of ethanol fermentation and its regulation in land plants. J Exp Bot. 2019 Feb 28. pii: erz052. doi: 10.1093/jxb/erz052.De la Rosa et al. (2019), A dicistronic precursor encoding miR398 and the legume-specific miR2119 coregulates CSD1 and ADH1 mRNAs in response to water deficit. Plant Cell Environ. 2019 Jan;42(1):133-144. doi: 10.1111/pce.13209. |
| Special application note: |
This product can be sold containing ProClin if requested |
| UniProt number: |
P06525 |
| TAIR number: |
AT1G77120 |
| Research area: |
Arabidopsis thaliana antibodies |
| Code: |
Stress20 |
|
