| Background Info: |
Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c?-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized |
| Storage Temp: |
Lyophilized antibody can be stored at -20 °C for up to 3 years. Re-constituted antibody can be stored at 4°Cfor several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana, Echinochloa crus-galli, Nannochloropsis oceanica strain IMET1, Panicum miliaceum, Pisum sativum, Zea mays |
| Expected Species: |
Acacia prainii, Angelphytum bahiense, Aristolochia promissa, Chlamydomonas reinhardii, Daucus glochidiatus, Dimerostemma brasilianum, Elaphandra paucipunctata, Fraxinus chiisanensis, Gossypium populifolium, Hydrangea heteromalla, Nicotiana sylvestris, Oryza sativa, Salix tetrasperma, Solanum lycopersicum, Thottea siliquosa, Triticum turgidum, Ulva flexuosa, Wedelia biflora, Vanilla aphyllaSpecies of your interest is not listed? Species of your interest not listed? Contact us. Species of your interest not listed? Contact us |
| Immunogen: |
KLH-conjugated peptide chosen from Arabidopsis thaliana PetB protein sequence, UniProt: P56773, TAIR: ATCG00720 |
| Applications: |
Blue native PAGE (BN-PAGE), Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
1 : 1000 - 1: 5000 (WB) |
| Purity: |
Serum |
| Reconstitution: |
For reconstitution add 50 l, of sterile water |
| Molecular Weight: |
24 kDa |
| Not reactive in: |
Chlamydomonas reihardtii |
| Selected references: |
Urban, Rogowski & Romanowska (2022), Crucial role of the PTOX and CET pathways in optimizing ATP synthesis in mesophyll chloroplasts of C3 and C4 plants, Environmental and Experimental Botany, Volume 202, October 2022, 105024, https://doi.org/10.1016/j.envexpbot.2022.105027Wada et al. (2021) Identification of a Novel Mutation Exacerbated the PSI Photoinhibition in pgr5/pgrl1 Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis pgr5-1 Mutant. Cells. 2021 Oct 26;10(11):2884. doi: 10.3390/cells10112884. PMID: 34831107; PMCID: PMC8616342.Chen et al. (2021)Degradation of the photosystem II core complex is independent of chlorophyll degradation mediated by Stay-Green Mg2+ dechelatase in Arabidopsis,Plant Science,Volume 307,2021,110902,ISSN 0168-9452,https://doi.org/10.1016/j.plantsci.2021.110902. Lu et al. (2021). Role of an ancient light-harvesting protein of PSI in light absorption and photoprotection. Nat Commun. 2021 Jan 29;12(1):679. doi: 10.1038/s41467-021-20967-1. PMID: 33514722; PMCID: PMC7846763.Wang et al. (2020). Post-translational coordination of chlorophyll biosynthesis and breakdown by BCMs maintains chlorophyll homeostasis during leaf development. Nat Commun. 2020; 11: 1254. |
| UniProt number: |
P56773 |
| TAIR number: |
ATCG00720 |
| Research area: |
Electron transfer |
| Code: |
Photo20 |
|
| Background Info: |
Phytochrome A (PhyA) is the primary photoreceptor mediating various responses to far-red (FR) light in plants. Alternative protein names: FAR RED ELONGATED HYPOCOTYL 2, FAR RED ELONGATED 1, ELONGATED HYPOCOTYL 8. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized |
| Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana, Arachis hypogaea L., Nicotiana benthamiana, Nicotiana tabacum |
| Expected Species: |
Brassica rapa, Cardamine hirsuta, Dacucus carota, Lathyrus sativus, Fragaria ananassa, Glycine max, Gossyoium hirsutum, Hordeum vulgare, Lotus corniculatus, Medicago truncatula, Nicotiana benthamiana (PhyA1), Nicotiana tabacum, Pisum sativum, Populus balsamifera, Ricinus communis, Solanum lycopersicumSpecies of your interest not listed? Contact us |
| Immunogen: |
KLH-conjugated synthetic peptide derived from conserved plant PhyA protein sequences including Arabidopsis thaliana UniProt:P14712, TAIR:At1g59070; peptide sequence is not present in other plant phytochrome forms (B-E) |
| Applications: |
Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
Careful sample collection is adviced to assure the best results with this antibody |
| Application Details: |
1 : 1000 (WB) |
| Purity: |
Immunogen affinity purified serum in PBS pH 7.4. |
| Reconstitution: |
For reconstitution add 50 l of sterile water |
| Molecular Weight: |
124 kDa |
| Not reactive in: |
No confirmed exceptions from predicted reactivity are currently known |
| Selected references: |
Schwenk et al. (2021) Uncovering a novel function of the CCR4-NOT complex in phytochrome A-mediated light signalling in plants. Elife. 2021 Mar 30;10:e63697. doi: 10.7554/eLife.63697. PMID: 33783355; PMCID: PMC8009681.Schenk et al. (2021) Light-induced degradation of SPA2 via its N-terminal kinase domain is required for photomorphogenesis, Plant Physiology, 2021;, kiab156, https://doi.org/10.1093/plphys/kiab156Menon et al. (2019). Arabidopsis FAR-RED ELONGATED HYPOCOTYL 1 and FHY1-LIKE are not required for phytochrome A signal transduction in the nucleus. Plant Communications. Available online 9 November 2019, 100007.Agliassa et al. (2018). Geomagnetic field impacts on cryptochrome and phytochrome signaling. J Photochem Photobiol B. 2018 Aug;185:32-40. doi: 10.1016/j.jphotobiol.2018.05.027.Zhang et al. (2018). Characterization of peanut phytochromes and their possible regulating roles in early peanut pod development. PLoS One. 2018 May 25;13(5):e0198041. doi: 10.1371/journal.pone.0198041. |
| Special application note: |
In vivo pull down assay for PhyA and western blot analysis of eluted proteins is described in Paik et al. (2012). Phytochrome regulates translation of mRNA in the cytosol. PNAS 109 (4): 1335-1340. |
| UniProt number: |
P14712 |
| TAIR number: |
AT1G09570 |
| Research area: |
Arabidopsis thaliana antibodies |
|
