| Background Info: |
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER, BiP protein is abundant under all growth conditions, but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Liquid in PBS pH 7,4. |
| Storage Temp: |
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana, Brassica napus, Chlamydomonas reinhardtiiCucumis sativus, Nicotiana benthamiana, Raphanus sativa L, Tokinashi-daikon, Olea europaea, Picea abies, Physcomitrella patens, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Zeay mays |
| Expected Species: |
Arabis alpina, Capsella rubella, Capsicum annuum, Citrus clementina, Citrus sinsensis, Eucalyptus grandis, Glycine max, Hordeum vulgare, Isatis tincorina, Prunus persica, Triticum aestivium, Petunia hybrida, Picea sitcHensis, Populus trichocarpa, Ricinus comminus, Vitis viniferaSpecies of your interest not listed? Contact us |
| Immunogen: |
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3, BiP2 At5g42020 F4K007 , BiP3 At1g09080 Q8H1B3 |
| Applications: |
Immunofluorescence (IF), Western Blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel, This antibody has so far not worked in IP. |
| Application Details: |
To be determined by end user |
| Purity: |
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 650. |
| Molecular Weight: |
73,5 | 80 kDa |
| Not reactive in: |
No confirmed exceptions from predicted reactivity are currently known. |
| Selected references: |
To be added when available. Antibody released in May 2023. |
| Special application note: |
DyLight 650 has Amax = 652 nm, Emax = 672 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries. |
| UniProt number: |
Q39043 |
| TAIR number: |
AT5G42020, AT5G42020 |
| Research area: |
Arabidopsis thaliana antibodies |
| Code: |
Primconj10 |
|
| Background Info: |
CAH3 - carbonic anhydrase is a zinc-containing metalloenzyme that catalyzes the reversible interconversion of CO2 and HCO3?. It plays an important role in many physiological functions that involve decarboxylation or carboxylation reactions, including both photosynthesis and respiration. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized |
| Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Chlamydomonas reinhardtii |
| Expected Species: |
Chlamydomonas reinhardtii (only) |
| Immunogen: |
Recombinant carbonic anhydrase of Chlamydomonas reinhardtii A8J4Z8 after cleavage of a fusion protein |
| Applications: |
immunofluorescence (IF), Immunogold (IG), Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
1 : 200 (IF), 1 : 2000 (WB) |
| Purity: |
Serum |
| Reconstitution: |
For reconstitution add 100 l of sterile water |
| Molecular Weight: |
33,4 | 29 kDa |
| Not reactive in: |
Arabidopsis thaliana, Oryza sativa |
| Selected references: |
Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9Shukshina &Terentyev (2021) Involvement of Carbonic Anhydrase CAH3 in the Structural and Functional Stabilization of the Water-Oxidizing Complex of Photosystem II from Chlamydomonas reinhardtii. Biochemistry Moscow 86, 867–877 (2021). https://doi.org/10.1134/S0006297921070075Terentyev (2020: The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated From Wild Type and cia3 Mutant Chlamydomonas reinhardtii. Life (Basel). 2020 May 14;10(5):E63. doi: 10.3390/life10050063.Terentyev et al. (2019). Carbonic anhydrase CAH3 supports the activity of photosystem II under increased pH. Biochim Biophys Acta Bioenerg. 2019 Jul 1;1860(7):582-590. doi: 10.1016/j.bbabio.2019.06.003.Correa-Galvis et al. (2016). Photosystem II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii. J Biol Chem. 2016 Aug 12;291(33):17478-87. doi: 10.1074/jbc.M116.737312. |
| Special application note: |
This antibody recognizes native and recombinant carbonic anhydrase of Chlamydomonas reinhardtii. |
| UniProt number: |
A8J4Z8 |
| Research area: |
Chlamydomonas reinhardtii antibodies |
| Code: |
Photo20 |
|
