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Product Search Results: 1111-01

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Product Search Results: 1111-01

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Agrisera AS12 1854 50 µg £317.00
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Background Info: NPR1 (regulatory protein NPR1) is a key positive regulator of the SA-dependent signaling pathway that negatively regulates JA-dependent signaling pathway. Controls the onset of systemic acquired resistance (SAR). Subcellular localization: cytoplasm, nucleaus (following induction by salicylic acid treatment or after pathogen infection. Alternative names: BTB/POZ domain-containing protein NPR1, Non-inducible immunity protein 1, Nim1, Salicylic acid insensitive 1, Sai1, ATNPR1.
Product Type: Antibody
Antibody Type: Polyclonal
Format: Lyophilized
Storage Temp: Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal: Rabbit
Species Reactivity: Arabidopsis thaliana
Expected Species: Arabidopsis thaliana
Immunogen: KLH-conjugated peptide, chosen from NPR1 sequence of Arabidopsis thaliana, TAIR: AT1G64280, UniProt: P93002
Applications: Western blot (WB)
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
Additional Info: Please note that depending upon detection system you are using, longer exposure time may be required with this antibody, Chemiluminescent detection reagent in extreme femtogram range is necessary
Application Details: 1 : 1000 (WB)
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution: For reconstitution add 50 l of sterile water
Molecular Weight: 66 | 66 kDa
Not reactive in: Marchantia sp. ,Nicotiana benthamiana, Nicotiana tabacum, Solanum lycopersicum, Solanum tuberosum
Selected references: Arenas-Alfonseca et al. (2021) Arabidopsis beta-cyanoalanine synthase mutation overcomes NADPH oxidase action in response to pathogens. J Exp Bot. 2021 Mar 26:erab137. doi: 10.1093/jxb/erab137. Epub ahead of print. PMID: 33770168.Nomoto et al. (2021) Suppression of MYC transcription activators by the immune cofactor NPR1 fine-tunes plant immune responses. Cell Rep. 2021 Dec 14;37(11):110125. doi: 10.1016/j.celrep.2021.110125. PMID: 34910911.Lei et al. (2020). Construction of gold-siRNANPR1 nanoparticles for effective and quick silencing of NPR1 in Arabidopsis thaliana. DOI: 10.1039/D0RA02156C (Paper) RSC Adv., 2020, 10, 19300-19308
Special application note: For successful detection using NPR1 antibody please follow protocol suggested below. NPR1 protein readily oligomerizes, in addition to any naturally occurring oligomer, during extraction. Therefore 50 mM DTT has to be used as well as denaturation at 75 C for 15 minutes. Engogenous NPR1 level is very low, thereofre SA treatment is absolutely necessary for good detection.This antibody is recognizing NPR1-GFP in the 35S overexpression line.
UniProt number: P93002
TAIR number: AT1G64280
Research area: Arabidopsis thaliana antibodies
Code: Stress20