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Product Search Results: AS19 4297

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Product Search Results: AS19 4297

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Agrisera AS19 4297 50 µl £277.00
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Background Info: NdbA (Thylakoid Localized Type 2 NAD(P)H Dehydrogenase) is localized to the thylakoid membrane and expressed at a very low level in the widl type under photoautotrophic growth. NdbA is induced substantially under light-activated heterotophic growth conditions.
Antibody Type: Polyclonal
Format: Lyophilized
Storage Temp: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal: Rabbit
Species Reactivity: Synechocystis sp. PCC 68
Expected Species: Bacillus subtilis
Immunogen: KLH-conjugated peptide derived from NdbA protein sequence of Synechocystis sp. PCC 6803, UniProt:P73739 Chosen peptide is not conserved in NdbB and NdbC.
Applications: Western blot (WB)
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
Additional Info: Protein extraction: harvested cells were suspended in a buffer containing 50 mM Hepes-NaOH, pH 7.5, 30 mM CaCl2, 800 mM sorbitol, 1 mM ε-amino-n-caproic acid, and the cells were broken by vortexing 6×1 min at 4°C in the presence of glass beads. Protein samples were solubilized in Laemmli buffer containing 6 M urea and separated on a gel in presence of 6M urea.
Application Details: 1 : 2000 (WB)
Purity: Serum
Reconstitution: For reconstitution add 50 µl, of sterile water.
Related products: collection of antibodies to cyanobacterial proteins | Photosynthesis antibodies
Molecular Weight: 49 | 46 kDa
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Selected references: Huokko et al. (2019). Thylakoid Localized Type 2 NAD(P)H Dehydrogenase NdbA Optimizes Light-Activated Heterotrophic Growth of Synechocystis sp. PCC 6803. Plant Cell Physiol. 2019 Mar 7. pii: pcz044. doi: 10.1093/pcp/pcz044.
UniProt number: UniProt