| Background Info: |
Goat anti-human IgG Fc, is an adsorbed F(ab)'2 fragment of secondary antibody conjugated to Rhodamine (TRITC) which binds to human IgG Fc part. Antibodies are conjugated to tetramethylrhodamine-5-isothiocyanate (TRITC). Amax = 550 nm, Emax = 570 nm. Purity is >90% based on SDS-PAGE. Small amounts of intact IgG may be present. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Liquid |
| Storage Temp: |
Store at 2-8°C. Shelf life of this product is one year from date of receipt. |
| Host Animal: |
Goat |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
The optimal working dilution should be determined by the investigator, |
| Purity: |
Immunogen affinity purified goat IgG, F(ab)'2 fragment. |
| Special application note: |
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains on human immunoglobulins or to non-immunoglobulin human serum proteins based on immunoelectrophoresis.No reactivity is observed to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative. |
| Research area: |
Fluorescent |
|
| Background Info: |
Rieske Iron-Sulfur Protein (Q9ZR03) is located in chloroplast thylakoid membrane as a component of cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. Alternative names: Rieske iron-sulfur protein, RISP, ISP, plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein, proton gradient regulation protein 1 |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized |
| Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana, Brassica rapa subsp. chinensis, Chlamydomonas reinhardtii, Echinola crus-galli, Euglena sp., Haematococcus pluvialis, Nicotiana tabacum, Panicum miliaceum, Pisum sativum, Spinacia oleracea, Synechococcus PCC 7942, Synechocystis sp. PCC 6803, Thalassiosira guillardii, Zea mays |
| Expected Species: |
Acetabularia acetabulum, Brachypodium distachyon, cyanobacteria, Calothrix sp. PCC 7507, Catalpa bungei, Cicer arietinum, Crocosphaera watsonii, Cynodon dactylon, Gossypium raimondii , Hordeum vulgare, Lyngbya aestuarii,Microcystis aeruginosa, Nannochloropsis gaditana, Nicotiana benthamiana, Pisum sativum, Ricinus communis , Saccharum hybrid cultivar ROC22, Selaginella moellendorffii, Solanum tuberosum, Sorghum bicolor, Oryza sativa, Physcomitrium patens, Phormidesmis priestleyi, Populus trichocarpa, Sonneratia alba, Triticum aestivum, Zostera marina, Vitis viniferaSpecies of your interest not listed? Contact us |
| Immunogen: |
KLH-conjugated synthetic peptide which shows strong conservation across higher plants including Arabidopsis thaliana UniProt:Q9ZR03, TAIR:At4g03280, Chlamydomonas reinhardtii P49728 and Synechococcus sp. Q5N5B0 |
| Applications: |
Blue Native PAGE (BN-PAGE), Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
1 : 5000-1 : 10 000 (BN-PAGE), (WB) |
| Purity: |
Serum |
| Reconstitution: |
For reconstitution add 50 l of sterile water |
| Molecular Weight: |
23 kDa |
| Not reactive in: |
Candidia albicans |
| Selected references: |
Pipitone et al. (2021). A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. Elife. 2021 Feb 25;10:e62709. doi: 10.7554/eLife.62709. PMID: 33629953; PMCID: PMC7906606.Kana et al. (2020). Fast Diffusion of the Unassembled PetC1-GFP Protein in the Cyanobacterial Thylakoid Membrane. Life (Basel). 2020 Dec 29;11(1):E15. doi: 10.3390/life11010015. PMID: 33383642.Zhang et al. (2020). Enhanced Relative Electron Transport Rate Contributes To Increased Photosynthetic Capacity In Autotetraploid Pak Choi. Plant Cell Physiol. 2020 Jan 6. pii: pcz238. doi: 10.1093/pcp/pcz238.Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4. Koochak et al. (2019). The structural and functional domains of plant thylakoid membranes. Plant J. 2019 Feb;97(3):412-429. doi: 10.1111/tpj.14127. |
| Special application note: |
This product can be sold containing Proclin if requested. |
| UniProt number: |
Q9ZR03 , P49728 , Q5N5B0 |
| TAIR number: |
AT4G03280 |
| Research area: |
Global Antibodies |
| Code: |
Photo20 |
|
