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Product Search Results: SMC-154D

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Product Search Results: SMC-154D

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StressMarq SMC-154D 100 µg £272.00
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Product Detail: This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase.
Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).
Product Type: Antibodies
Format: PBS, 50% glycerol, 0.09% sodium azide
Storage Temp: -20ºC
Host Animal: Mouse
Species Reactivity: ALL
Immunogen: 3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin
Applications: WB | IHC | ICC/IF | IP | ELISA | FCM | AM
Clone number: 39B6
Antibody Isotype: IgG2a
Application Details: WB (1:1400), IHC (1:100); optimal dilutions for assays should be determined by the user.
Research Area(s): Cancer | Oxidative Stress | Cell Signaling | Post-translational Modifications | Nitration | Neuroscience | Neurodegeneration | Alzheimer's Disease | Parkinson's Disease
Target: Nitrotyrosine
Conjugate: Unconjugated
Alternative Name(s): Nitro tyrosine Antibody, 3-Nitrotyrosine Antibody
Category: Monoclonal
Species Reactivity Full Name: Species Independent
Purification: Protein G Purified
Concentration: 1 mg/ml
Specificity: Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.
Certificate of Analysis: 0.7 µg/ml of SMC-154 was sufficient for detection of 5 µg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
References: 1. Girault I. et al. (2001). Free Radical Biology and Medicine, 31 (11): 1375-1387.
2. Gow AJ, Farkouh CR, Munson DA, Posencheq MA, and Ischiropoulos H. (2004). Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.
3. Takemoto K. et al (2007). Acta Med Okayama 61(1): 17-30.
4. Reynolds MR. et al. (2006) J Nerosci. 26(42): 10636-45.
5. Pfister H., et al. (2002) Vet Pathol. 39: 190-199.
6. Khan J. et al. (1998) Biochem J. 330(2): 795-801.
Tarriff Code: 3002.15.0000
ADR Code: Non-hazardous
UN Code for transport: Non-hazardous
Country of Origin: Canada
Cite this Product: StressMarq Biosciences Cat# SMC-154D, RRID: AB_904533