Product Search Results : NOVA-4245-05

Num PRODUCTS MATCHED : 1

Product - please click for more details Brand Cat No Size Price (£) ▲

Bioo Scientific NOVA-4245-05 96 rxns £4805.00
Please sign in or sign up to order this product online.
Product Detail: The NEXTflex™ Congenital Hyperinsulinism Amplicon Panel allows you to quickly target and sequence 119 exons of the ABCC8, GLUD1, KCNJ11, GCK, HADH, HNF4A, INS, INSR, PDX1, SLC16A1 and UCP2 loci and two pathological mutations in the SLC16A1 promoter and one pathological mutation in HADH intron 6 region. This kit contains primer pairs and reagents needed to amplify these coding regions of the ABCC8, GLUD1, KCNJ11, GCK, HADH, HNF4A, INS, INSR, PDX1, SLC16A1 and UCP2 genes and two pathological mutations in the SLC16A1 promoter and one pathological mutation in HADH intron 6 region from DNA isolated from fresh or frozen material, and prepare libraries for Illumina® sequencing.

While up to 384 barcodes are currently supplied with the NEXTflex Congenital Hyperinsulinism Amplicon Panel, the panel has the capability of multiplexing up to 180 samples at 100x coverage for detection of germline mutations on a single Illumina® 2x300 MiSeq® lane.


[BENEFITS]
• 100% coverage of ABCC8, GLUD1, KCNJ11, GCK, HADH, HNF4A, INS, INSR, PDX1, SLC16A1 and UCP2 exons and flanking intron-exon boundaries
• 98% uniformity of amplicons on 0.2x coverage; 100% of amplicons detected at >0.1X mean coverage
• 384 unique barcodes available
• Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
• Low input - Only 20 ng DNA isolated from fresh or frozen samples required
Product Type: NGS - Illumina Amplicon Panels
Applications: Congenital Hyperinsulinism Amplicon Panel
Search Terms: Illumina Congenital Hyperinsulinism Amplicon Panel
Application Details: Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update: Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4245-05
NGS Applications: Congenital Hyperinsulinism Amplicon Panel