| Product Detail: |
Anti-WNK1 (Clone M42-P3B10) |
| Background Info: |
WNK1 is a cytoplasmic serine-threonine kinase involved in the regulation of electrolyte homeostasis, cell signalling, survival, and proliferation. It is part of the ERK5 MAP kinase pathway and acts as an activator of sodium-coupled chloride cotransporters such as SCNN1A, SCNN1B, SCNN1D and SGK1 and as an inhibitor of WNK4 by phosphorylation or through an interaction with the WNK1 autoinhibitory domain. WNK1 may also play a role in actin cytoskeletal reorganisation.
Defects in WNK1 are a cause of Gordon hyperkalemia-hypertension syndrome (pseudohypoaldosteronism type II - PHAII) an autosomal dominant disease characterised by severe hypertension and sensory and autonomic neuropathy type 2A (HSAN2A), a form of congenital sensory and autonomic neuropathy.
Homologs of this WNK1 have been found in Arabidopsis thaliana, C. elegans, Chlamydomonas reinhardtii and Vitis vinifera as well as in vertebrates including Danio rerio and Taeniopygia guttata.
This antibody was validated with HEK 293T cell lysate and HeLa cell lysate (1 in 1000 in 5% BSA) |
| Product Type: |
NS Reagents Antibody |
| Antibody Type: |
Monoclonal |
| Format: |
100 µg in 100 µl PBS containing 0.02% sodium azide. |
| Storage Temp: |
6 months: 4°C. Long-term storage: -20°C. Avoid multiple freeze and thaw cycles. |
| Host Animal: |
Mouse |
| Species Reactivity: |
Human |
| Expected Species: |
Mouse , Rat, Chicken, Duck, Hamster, Goat, Sheep, Cow, Dog, Cat, Pig and Horse.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you. |
| Immunogen: |
A synthetic peptide from the C-terminal region of Human WNK1 - Ovalbumin Conjugated (The selected sequence is highly conserved across many species). |
| Applications: |
ELISA ¦ IHC ¦ WB |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
If you would like further information regarding the immunogen used in the productiion of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you. |
| Clone number: |
Clone M42-P3B10 |
| Antibody Isotype: |
IgG1 kappa |
| Application Details: |
WB 1:1000 in 5% BSA, IHC 1:50-1:500 |
| Antibody Number: |
10003 |
| Category: |
Primary Antibodies |
| Other names: |
WNK1, Erythrocyte 65 kDa protein, Human Accelerated Region 5, WNK Lysine Deficient Protein Kinase 1, hWNK1 |
| Research Areas: |
Cell Signalling, Neuroscience |
| NS Reagents Product Area: |
Cell Signalling |
| Molecular Weight: |
65kDa (Intended as a general guide and does not allow for all isoforms and species variations) |
| Subcellular location: |
Cytoplasm |
| Purification: |
Protein A Affinity Purified |
| Uniprot ID: |
Q9H4A3 |
| Gene ID: |
65125 |
| Gene symbol: |
WNK1 |
| Release Date: |
Oct-17 |
