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Product Search Results: 1411-01
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Product Search Results: 1411-01

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Agrisera AS99 001 50 µg £314.00
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Background Info: Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host’s immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.
Product Type: Antibody
Antibody Type: Polyclonal
Format: Lyophilized
Storage Temp: Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal: Rabbit
Species Reactivity: Megathura crenulata - most commonly used carrier protein
Immunogen: Purified keyhole limpet hemocyanin (KLH), whole molecule,
Applications: ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
Additional Info: Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies, Due to its large size KLH protein will be very difficult to separate on SDS-PAGE
Application Details: 1: 10 000 (ELISA), 1: 10 000 (WB), 1: 1000 (IL)
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution: For reconstitution add 50 l of sterile water
Molecular Weight: ca, 400 kDa/subunit
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Selected references: Geadkaew et al. (2014). Bi-functionality of Opisthorchis viverrini aquaporins. doi:10.1016/j.biochi.2014.11.013.H glund et al. (2002). An Antigen Expressed During Plant Vascular Development Crossreacts with Antibodies Towards KLH (Keyhole Limpet Hemocyanin). J of Histochem & Cytochem. 50:999-1003.
Special application note: Protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (H glund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide.
UniProt number: Q6KC56
Research area: Carrier Proteins