The NEXTflex Bisulfite-Seq Kits contain enough material to prepare 8 or 48 genomic DNA samples for Illumina compatible sequencing.
The shelf life of all reagents is 12 months when stored properly.
DNA Binding Buffer, DNA Wash Buffer and the Clean-Up Spin Columns should be stored at room temperature. All of the other components can be safely stored at -20°C.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex Bisulfite-Seq Kit is designed work with reduced representation or total bisulfite-converted DNA libraries and is compatible with single, paired-end and multiplexed DNA libraries on Illumina MiSeq, GAIIx and HiSeq platforms. This kit features Enhanced Adapter Ligation Technology resulting in library preps with a larger number of unique sequencing reads. This specially designed NEXTflex ligation enzymatic mix allows users to perform ligations with longer adapters and better ligation efficiencies. The NEXTflex Bisulfite-Seq Kit is a versatile kit designed to facilitate assessment of the methylation state of the genome and simplify workflow by using master mixed reagents, magnetic bead based cleanup, which reduce pipetting and eliminate time consuming steps in library preparation. In addition, the availability of the methylated NEXTflex Bisulfite-Seq barcoded adapters make multiplexing possible.
The NEXTflex Bisulfite-Seq Kit with reduced representation utilizes a restriction enzyme that leads to selective amplification of CpG regions, resulting in focused sequence depth. The NEXTflex Msp1 Restriction Enzyme, which is available separately, has been optimized for reduced representation studies when used in conjunction with the NEXTflex Bisulfite-Seq Kit. The NEXTflex Bisulfite-Seq Kit can also be used without the MSP 1 Restriction Enzyme for genome-wide methylation-seq analysis, including under-represented CpG regions.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[KIT FEATURES] >>> Compatible with total bisulfite sequencing and reduced representation >>> Single nucleotide resolution of methylation sites >>> Uracil insensitive polymerase designed for bisulfite-converted DNA >>> Methylome-level assessment with broad genome coverage >>> Enhanced adapter ligation technology with NEXTflex Ligation >>> Bead-based cleanup >>> Automation-friendly workflow is compatible with liquid handlers >>> Methylated barcoded adapters for multiplexing contain embedded index sequence >>> Functionally validated with Illumina MiSeq, GAIIx and HiSeq platforms
Application Details:
Please use our contact form above to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5119-01
The NEXTflex Bisulfite-Seq Kits contain enough material to prepare 8 or 48 genomic DNA samples for Illumina compatible sequencing.
The shelf life of all reagents is 12 months when stored properly.
DNA Binding Buffer, DNA Wash Buffer and the Clean-Up Spin Columns should be stored at room temperature. All of the other components can be safely stored at -20°C.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex Bisulfite-Seq Kit is designed work with reduced representation or total bisulfite-converted DNA libraries and is compatible with single, paired-end and multiplexed DNA libraries on Illumina MiSeq, GAIIx and HiSeq platforms. This kit features Enhanced Adapter Ligation Technology resulting in library preps with a larger number of unique sequencing reads. This specially designed NEXTflex ligation enzymatic mix allows users to perform ligations with longer adapters and better ligation efficiencies. The NEXTflex Bisulfite-Seq Kit is a versatile kit designed to facilitate assessment of the methylation state of the genome and simplify workflow by using master mixed reagents, magnetic bead based cleanup, which reduce pipetting and eliminate time consuming steps in library preparation. In addition, the availability of the methylated NEXTflex Bisulfite-Seq barcoded adapters make multiplexing possible.
The NEXTflex Bisulfite-Seq Kit with reduced representation utilizes a restriction enzyme that leads to selective amplification of CpG regions, resulting in focused sequence depth. The NEXTflex Msp1 Restriction Enzyme, which is available separately, has been optimized for reduced representation studies when used in conjunction with the NEXTflex Bisulfite-Seq Kit. The NEXTflex Bisulfite-Seq Kit can also be used without the MSP 1 Restriction Enzyme for genome-wide methylation-seq analysis, including under-represented CpG regions.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[KIT FEATURES] >>> Compatible with total bisulfite sequencing and reduced representation >>> Single nucleotide resolution of methylation sites >>> Uracil insensitive polymerase designed for bisulfite-converted DNA >>> Methylome-level assessment with broad genome coverage >>> Enhanced adapter ligation technology with NEXTflex Ligation >>> Bead-based cleanup >>> Automation-friendly workflow is compatible with liquid handlers >>> Methylated barcoded adapters for multiplexing contain embedded index sequence >>> Functionally validated with Illumina MiSeq, GAIIx and HiSeq platforms
Application Details:
Please use our contact form above to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5119-02
The NEXTflex Bisulfite-Seq Barcodes Kits contain 6 12 or 24 unique barcodes, enabling the user to multiplex up to 24 samples per flow cell lane.
These kits ship on dry ice.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - Bisulfite-Seq - Illumina Compatible
Applications:
Bisulfite-Seq, Methyl-Seq
Search Terms:
Bisulfite-Seq
Additional Info:
The NEXTflex Bisulfite-Seq Barcodes are designed to be used with the NEXTflex Bisulfite-Seq Kit. Unlike other NEXTflex Barcodes, the NEXTflex Bisulfite-Seq Barcodes are methylated. The NEXTflex Bisulfite-Seq Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex Bisulfite-Seq Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR.
These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex Bisulfite-Seq Kits.
[KIT FEATURES] >>> Index contained within adapter sequence >>> Up to 24 methylated, multiplexed adapters are available >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAIIx, HiSeq and MiSeq)
Application Details:
Please use our contact form above to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 511911
The NEXTflex Bisulfite-Seq Barcodes Kits contain 6 12 or 24 unique barcodes, enabling the user to multiplex up to 24 samples per flow cell lane.
These kits ship on dry ice.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - Bisulfite-Seq - Illumina Compatible
Applications:
Bisulfite-Seq, Methyl-Seq
Search Terms:
Bisulfite-Seq
Additional Info:
The NEXTflex Bisulfite-Seq Barcodes are designed to be used with the NEXTflex Bisulfite-Seq Kit. Unlike other NEXTflex Barcodes, the NEXTflex Bisulfite-Seq Barcodes are methylated. The NEXTflex Bisulfite-Seq Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex Bisulfite-Seq Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR.
These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex Bisulfite-Seq Kits.
[KIT FEATURES] >>> Index contained within adapter sequence >>> Up to 24 methylated, multiplexed adapters are available >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAIIx, HiSeq and MiSeq)
Application Details:
Please use our contact form above to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 511912
The NEXTflex Bisulfite-Seq Barcodes Kits contain 6 12 or 24 unique barcodes, enabling the user to multiplex up to 24 samples per flow cell lane.
These kits ship on dry ice.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - Bisulfite-Seq - Illumina Compatible
Applications:
Bisulfite-Seq, Methyl-Seq
Search Terms:
Bisulfite-Seq
Additional Info:
The NEXTflex Bisulfite-Seq Barcodes are designed to be used with the NEXTflex Bisulfite-Seq Kit. Unlike other NEXTflex Barcodes, the NEXTflex Bisulfite-Seq Barcodes are methylated. The NEXTflex Bisulfite-Seq Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex Bisulfite-Seq Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR.
These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex Bisulfite-Seq Kits.
[KIT FEATURES] >>> Index contained within adapter sequence >>> Up to 24 methylated, multiplexed adapters are available >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAIIx, HiSeq and MiSeq)
Application Details:
Please use our contact form above to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 511913
SHELF LIFE: The shelf life of MspI is 12 months when stored under optimal conditions. Avoid repeated freeze thaw cycles
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - Bisulfite-Seq - Illumina Compatible
Applications:
Bisulfite-Seq
Search Terms:
Bisulfite-Seq
Additional Info:
NEXTflex Msp1 Restriction Enzyme is a restriction enzyme that leads to selective amplification of CpG regions, resulting in focused sequence depth.
The NEXTflex Msp1 Restriction Enzyme is designed to be used with the NEXTflex Bisulfite-Seq Kit for reduced representation. Unlike other commercially available MSP1 restriction enzymes, the NEXTflex Msp 1 Restriction Enzyme has been optimized for bisulfite sequencing
[KIT FEATURES] >>> Optimised for bisulfite sequencing
Application Details:
Please use our contact form above to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 511921
SHELF LIFE: The shelf life of MspI is 12 months when stored under optimal conditions. Avoid repeated freeze thaw cycles
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - Bisulfite-Seq - Illumina Compatible
Applications:
Bisulfite-Seq
Search Terms:
Bisulfite-Seq
Additional Info:
NEXTflex Msp1 Restriction Enzyme is a restriction enzyme that leads to selective amplification of CpG regions, resulting in focused sequence depth.
The NEXTflex Msp1 Restriction Enzyme is designed to be used with the NEXTflex Bisulfite-Seq Kit for reduced representation. Unlike other commercially available MSP1 restriction enzymes, the NEXTflex Msp 1 Restriction Enzyme has been optimized for bisulfite sequencing
[KIT FEATURES] >>> Optimised for bisulfite sequencing
Application Details:
Please use our contact form above to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 511922
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