Background Info: |
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). |
Product Type: |
Antibody |
Antibody Type: |
Polyclonal |
Format: |
Lyophilized |
Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
Host Animal: |
Rabbit |
Species Reactivity: |
Arabidopsis thaliana, Hyacinthus orientalis, Nicotiana benthamiana |
Expected Species: |
Brassica pekinensis, Capsella rubella, Cucumis sativus, Glycine max, Malus domestica, Pisum sativum, Ricinus communis, Solanum tuberosum, Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us |
Immunogen: |
KLH-conjugated, N-terminal peptide of Arabidopsis thaliana AGO1 O04379, At1g48410 |
Applications: |
Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF), Immunolocalization (IL), small-RNA-IP-Seq, Western blot (WB) |
Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
Additional Info: |
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Buffer for extraction of AGO proteins: Paudel et al. 2018. The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.TCA acetone precipitation method |
Application Details: |
2 g (ChIP), 1: 200 (IF), (IL), small-RNA-IP-Seq, 1 : 5000-1 : 10 000 (WB) |
Purity: |
Immunogen affinity purified serum in PBS pH 7.4. |
Reconstitution: |
For reconstitution add 50 l of sterile water |
Molecular Weight: |
116,4 | 130 kDa |
Not reactive in: |
Chlamydomonas reinhardtii, Oryza sativa, Phaseolus vulgaris, Triticum aestivum, Zea mays |
Selected references: |
Meng et al. (2022) The novel activity of Argonautes in intron splicing: A transcriptome-wide survey in plants. J Plant Physiol. 2022 Jan 31;270:153632. doi: 10.1016/j.jplph.2022.153632. Epub ahead of print. PMID: 35114616.Cabezas-Fuster et al. (2022). Missplicing suppressor alleles of Arabidopsis PRE-MRNA PROCESSING FACTOR 8 increase splicing fidelity by reducing the use of novel splice sites. Nucleic Acids Res. 2022 Jun 10;50(10):5513-5527. doi: 10.1093/nar/gkac338. PMID: 35639749; PMCID: PMC9177961.Delenko et al. (2022) MicroRNA biogenesis and activity in plant cell dedifferentiation stimulated by cell wall removal. BMC Plant Biol. 2022 Jan 3;22(1):9. doi: 10.1186/s12870-021-03323-9. PMID: 34979922; PMCID: PMC8722089. (immunofluorescence)Oliver & Martinez. (2021) Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. Plant Reprod. 2021 Nov 23. doi: 10.1007/s00497-021-00434-z. Epub ahead of print. PMID: 34812935.Dalmadi et al. (2021) Controlled RISC loading efficiency of miR168 defined by miRNA duplex structure adjusts ARGONAUTE1 homeostasis. Nucleic Acids Res. 2021 Dec 16;49(22):12912-12928. doi: 10.1093/nar/gkab1138. PMID: 34850097; PMCID: PMC8682782. |
Special application note: |
Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.To detect AGO1 in Nicotiana benthamiana, please inquire.Recommended for detection of AGO1: extreme low femtogram range chemiluminescent detection reagent |
UniProt number: |
O04379 |
TAIR number: |
AT1G48410 |
Research area: |
Arabidopsis thaliana antibodies |