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Product Search Results: 2106-01

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Product Search Results: 2106-01

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Product - please click for more details Brand Cat No ▲ Size Price (£)

Agrisera AS09 458 50 µg £317.00
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Background Info: PEPC (phosphoenolpyruvate carboxylase), EC=4.1.1.31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate. Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3
Product Type: Antibody
Antibody Type: Polyclonal
Format: Lyophilized
Storage Temp: Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Please do not re-use this primary antibody solution. In case of cyanobacterial samples there will be no signal in your second incubation.
Host Animal: Rabbit
Species Reactivity: Ananas comosus, Arabidopsis thaliana, Cenchrus ciliaris, Chenopodium quinoa, Chloris gayana, Chromera velia, Cyanthobasis fruticulosa, Hordeum vulgare, Jatropha curcas, Kochia prostrata, Leptochloa fusca, Lupinus sp. , Megathyrsus maximus, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Panicum antidotale, Panicum coloratum, Petrosimonia nigdeensis, Pinus strobus, Saccharum spp. hybrid clone C91-301, Salsola lanata, Salsola laricifolia,Salsola grandis, Salsola tragus Sorghum bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), Pinus strobus, Thalassiosira weissfloggi, Zea mays, Zostera muelleri
Expected Species: Brassica napus, Cucumis sativus (PEPC1, PEPC2, PEPC3), Flaveria bidentis, Flaveria trinervia, Glycine max, Lupinus albus, Mammillaria thornberi, Manihot esculenta, Manihot obovata, Medicago sativa, Morinda citrifolia, Nannochloropsis gaditana CCMP526, Nopalea gaumeri, Opuntia macbridei, Pachycereus pringlei, Saccharum spp, Solanum tuberosum, Spinacia oleracea, Streptanthus tortuosus, Pachycereus hollianus, Pisum sativa, Phaseolus vulgaris, Populus sp.,Triticum aestivum, algae, diatoms: Thalassiosira pseudonana, other species: Salmonella sp., Schiedea hookeri, Shigella sp. Schiedea sarmentosa, Streptanthus farnsworthianus, Tacinga saxatilis,Yersinia sp. Vibrio sp., Quercus sp.Species of your interest not listed? Contact us
Immunogen: KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0, At1g53310 (PEPC 1), Q84VW9, At3g14940 (PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzyme NP_177043.2 (PEPC 4). For Zea mays, the peptide is converved in PEP1 and PEP4 isoforms.
Applications: Immunolocalization (IL), Western blot (WB)
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
Additional Info: Antibody can be also used following 2D gel electrophoresis
Application Details: 1 : 500 (IL), 1: 1000 - : 10 000 (WB)
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution: For reconstitution add 50 l of sterile water
Molecular Weight: 110 | 105 kDa
Not reactive in: Methanothermobacter thermautotrophicus
Selected references: Durall et al. (2021). Production of succinate by engineered strains of Synechocystis PCC 6803 overexpressing phosphoenolpyruvate carboxylase and a glyoxylate shunt. Microb Cell Fact. 2021 Feb 8;20(1):39. doi: 10.1186/s12934-021-01529-y. PMID: 33557832; PMCID: PMC7871529.Wang et al. (2021). Brassinosteroids inhibit miRNA-mediated translational repression by decreasing AGO1 on the endoplasmic reticulum. J Integr Plant Biol. 2021 May 21. doi: 10.1111/jipb.13139. Epub ahead of print. PMID: 34020507.Rakhmankulova et al. (2021) Possible Activation of ?3 Photosynthesis in ?4 Halophyte Kochia prostrata Exposed to an Elevated Concentration of ??2. Russ J Plant Physiol 68, 1107–1114 (2021). https://doi.org/10.1134/S1021443721060169Durall et al. (2020). Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803. Biotechnol Biofuels. 2020 Jan 28;13:16. doi: 10.1186/s13068-020-1653-y. Kramer et al. (2020). N6?methyladenosine and RNA secondary structure affect transcript stability and protein abundance during systemic salt stress in Arabidopsis. Plant Direct . 2020 Jul 24;4(7):e00239.doi: 10.1002/pld3.239.
UniProt number: Q9MAH0 , Q84VW9
TAIR number: AT1G53310, AT3G14940
Research area: Global Antibodies