The catenins, (alpha, beta and gamma) are cytoplasmic proteins which bind to the highly conserved tail of the E-cadherin molecule. Beta-catenin is a component of the adherens junction, a multiprotein complex which supports Ca2+ -dependent cell-to-cell contact, which in itself is critical for adhesion, signal transmission and for anchoring the actin cytoskeleton. Beta-catenin's role is as a transcription effector of the wnt-signaling pathway. Immunohistochemistry is the best way to demonstrate nuclear expression of beta-catenin and wnt-pathway activation. This aberrant expression is observed in human tumorigenesis, and especially in colorectal cancer.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
17C2
Concentration:
Greater than or equal to 51 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Curia MC et al. Modern Pathology. 2008; 21:7-14
References 2:
Ortega P et al. Clinical Cancer Research. 2008; 14(14):995-1001
References 3:
Daa T et al. J. of Exp.Clin.Cancer Research. 2005; 24(1):83-87
References 4:
Fadare O et al. World Journal of Surgical Oncology. 2005; 3(38)
References 5:
Gamachi A et al.Modern Pathology. 2003; 16(11):1124-1131
T-cell leukemia/lymphoma protein 1 (TCL1, TCL1A, p14TCL1) is a 14 kDa product of the TCL1 gene that is involved in T-cell prolymphocytic leukemia (T-PLL). TCL1 protein is normally found in the nucleus and cytoplasm of lymphoid lineage cells during early embryogenesis. TCL1 is expressed in differentiated Bcells under both reactive and neoplastic conditions, antigen committed B-cells, and in germinal center B-cells. The Anti-TCL1 immunohistochemical reactivity is reportedly useful identifying B-cell lymphomas including follicular lymphoma and Burkitt lymphoma.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-7
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Takizawa J, et al. Jpn J Cancer Res. 1998; 89:712-8
References 2:
Narducci MG, et al. Cancer Res. 2000; 60:2095-100
References 3:
Rodig SJ, et al. Am J Surg Pathol. 2008; 32:113-22
References 4:
Herling M, et al. Leukemia. 2006; 20:280-5
References 5:
Pescarmona E, et al. Histopathology. 2006; 49:343-8
SV40, Simian Virus 40 is a polyomavirus that is found in both monkeys and humans. Like other polyomaviruses, SV40 is a DNA virus that has the potential to cause tumors. SV40 is believed to suppress the transcriptional properties of tumor-suppressing p53 in humans through the SV40 large T-antigen and SV40 small T-antigen. It is generally assumed that large T-antigen is the major protein involved in neoplastic processes and the large T-antigen predominantly exerts its effect through deregulation of tumor suppressor p53, which is responsible for initiating regulated cell death (apoptosis), or cell cycle arrest when a cell is damaged. A mutated p53 gene may contribute to uncontrolled cellular proliferation, leading to a tumor.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-4
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Gurney, E.G., et al. J Virl. 34:752-763 (1980)
References 2:
Huang, H., Reis,R. et al. Brain Pathol., 9:33-42 (1999)
References 3:
Arrington, A.S., et al. Molecular and Clinical Perspectives; 461-489 (2001)
EBS-T-005 reacts with MADER or Melanoma-Associated Delayed Early Response protein, encoded by the NAB2 gene, belonging to the family of NGFI-A binding proteins. They modulate transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can form homo- or hetero-multimers with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains.
EBS-I-025 reacts with a conserved repeat domain on LMP1 (AA 268-286), present in all EBV isolates. LMP1 is expressed in most viral latency stages in vitro and in vivo
108-2C5 recognizes an intralocus determinant present on a limited number of HLA-A locusencoded gene products (HLA-A2, -A3, A28, -A29, -A30, -A31 and -Aw33). Furthermore, by testing its reactivity with HLA-A2 natural variants and mutants, the importance of amino acid residues 79 and/or 80 of the alpha1 domain was demonstrated in the formation of an intralocus HLA-A determinant.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
108-2C5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Lozano, F. et al., Immunogenetics 30: 50-53 (1989)
References 2:
Lozano, F. et al., Tissue Antigens 35: 193-195 (1990)
References 3:
Domenech, N. et al., Human Immunol 30: 140-146 (1991)
References 4:
Ryschich, E, et al, Clin Cancer Res. 11(2 Pt 1): 498-504 (2005)
SC-05 reacts with a reduction resistant epitope on 80 kDa human secretory component (both free and bound to SIgA). Secretory component is differentially expressed in epithelium, thus SC-05 can identify subpopulations of epithelial cells and epithelial differentiation. Secretory component negative cell lines are not stained with SC-05.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
SC-05
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Kvale, D. et al, Int J Cancer 42(4): 638-641 (1988)
References 2:
Bartek, J. et al, Histochem 91(3): 235-244 (1989)
References 3:
Bartek, J. et al, Histochem J 22(10): 537-534 (1990)
UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is a 1,416 amino acid nuclear membrane protein. It was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, a mRNA differentially expressed in F9 embryonal carcinoma cells, and that is up-regulated during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1- pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
AE-5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Yamada, K., et al. Biochem. Biophys. Res. Commun. 280: 1169-1176 (2001)
FR4A5 reacts with CD15 (220 kDa). CD15 contains the pentasaccharide lacto-n-fucopentaose III and plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance. It is also expressed on a wide variety of other tumor cells including myeloid leukemia, breast, colorectal, and lung cancer cells. Cross reactivity has been observed with Glc?1-6glc?1-4Glc, Tn, blood group H1, and maltose.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
FR4A5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Manimala J.C. et al. Glycobiology. 17(8): 17C-23C (2007)
References 2:
Gildersleeve J. et al. Glycobiology. 18(0): 746 (2008)
Laminins are large hetero-trimeric, non-collagenous glycoproteins found in basement membranes and composed of ?, ?, and ? chains. A5 reacts specifically with ? chain 1. Alterations of basement membrane integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
A5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Ljubimov JY et. al. Cancer Res. 61(14): 5601-5610 (2001)
References 2:
Ljubimov AV et. al. Int J Cancer 50: 562-566 (1992)
TV-1 recognizes fibronectin in frozen and paraffin sections of human, pig, mouse and rat tissues. Specifically, it stains this extracellular adhesive glycoprotein in connective tissues and blood vessels. TV-1 does not recognize the soluble dimeric form of fibronectin (plasma fibronectin) but strongly stains matrix fibronectin in tissues. Cellular fibronectin is widely distributed in the stroma of many malignant tumors
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
TV-1 (2755-8, EP-5)
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Epstein, AL. et al. Cancer Res. 55(12): 2673-80 (1995)
The antibody reacts with an internal epitope of MRP1, a 180-195 kD transmembrane transporter protein overexpressed in various human non-P-glycoprotein MDR tumor cell lines. MRPm5 was raised against a bacterial fusion protein of MRP1, containing amino acids 986-1204 of the protein. MRPm5 does not cross-react with the human MDR1 and MDR3 gene products.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MRPm5
Concentration:
100 ug/ ml
Format:
Protein G purified
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Cole S et al. Science 1992; 258: 1650-1654
References 2:
Flens M et al. Cancer Res 1994; 54: 4557-4563
References 3:
Zaman et al. Proc Nat Acad Sci 1994; 91: 8822-8826
VU-4H5 reacts with the protein core of MUC1, an apical cell side epithelial marker which is upregulated or switched on in the majority of carcinomas. The dominant epitope of VU-4H5 is PDTR, located in the VNTR domain of MUC1. In tissue sections, VU-4H5 also displays prominent staining of the cytoplasm.
EBS-CD-045 reacts with human CD100, a 150 kDa homodimer cell-surface antigen that is expressed on resting and PHA-stimulated T-cells. It is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. The protein is weakly expressed on NK-cells, EBV transformed B-cells, monocytes and tumor T-cell lines. It plays a role in homotypic cell adhesion and in T-cell activation.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-045
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Hall K, et al. P. Natl. Acad. Sci. USA 93: 11780 (1996)
References 2:
Mizrahi S, et al. PLoS One. 2(9): e818 (2007).
References 3:
Yoshino N, et al.. Exp. Anim. (Tokyo) 49: 97 (2000)
IBL-3/25 is directed against the ?-chain of CD8. The CD8 complex consists of a disulphide-linked ?/? heterodimer with a MW of 30 kDa or an ?/? homodimer with a MW of 32 kDa. The CD8 molecule binds to HLA class I molecules during interaction of CD8+ T-cells with antigenpresenting cells or with target cells. CD8+ T-cells include most of the cytotoxic T-cells.
Antibody Isotype:
IgG-K
Monosan Range:
MONOSAN
Clone:
IBL-3/25
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp W. et. al. Leukocyte Typing IV, p342-343, (1989)
References 2:
Parnes JR, Semin Immunol 6: 221-229 (1994)
References 3:
Delon J. et al. Immunity 9(4): 467-73 (1998)
References 4:
Akimoto H, et al. Immunology 95(2): 214-218 (1998)
EBS-CD-005 recognizes the CD6 molecule, a single chain transmembrane glycoprotein of 120 kDa, which is expressed on the majority of mature T-cells an mature thymocytes. A B-cell subset is weakly positive and B-CLLs may also be reactive. CD6 is a type 1 transmembrane glycoprotein that is tyrosine phosphorylated during TCR-mediated T-cell activation. CD6 shows significant homology to CD5. Antibodies to CD6 are used to deplete T-cells from bone marrow transplants to prevent graft versus host disease.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-CD-005
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Yssel et al., Cell. Immunol. 105: 161 (1987)
References 2:
Kamoun et al., J. Immunol. 127: 987 (1981)
References 3:
Reinherz et al., Proc. Nat. Acad. Sci. 79: 6047 (1982)
CD45 glycoprotein have various molecular weight on various cell types: B-cells 240 kDa, thymocytes 180 kDa, T-cells multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Isoforms are produced by alternative splicing of domains 4, 5 and 6. Various isoform are expressed differently on different lymphocytes. All hematopoietic cells express CD45 proteins except erythrocytes. Relevant epitopes are termed CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45R or CD45R0 (exon 4-6 spliced out).
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
Bra55
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Chorváth et al., Neoplasma 34(6), 685-692, (1987)
References 2:
Chorváth et al., Neoplasma 35(5), 495-501, (1988
References 3:
Chorváth et al. Leukocyte Typing IV, pp. 634-637, (1989)
EBS-CD-025 reacts with a 45 kDa glycopeptide which is a type II membrane glycoprotein with a transmembrane sequence near the regulation of lymphocyte adhesion to endothelial cells. Its ligand is CD31. CD38 is found on early cells of B and T lineages and on activated B- and tcells. It is not found on most mature resting peripheral lymphocytes. Also positive are thymus cells and Ig secreting plasma cells. The CD34+ and CD38- population of hematopoietic stems cells defines the most pluripotent cells (e.g. blast colony forming cells). EBS-CD-025 antibody blocks the EBS-CD-026 epitope, and vice versa.
It recognizes a transmembrane glycoprotein of 95 kDa, identified as CD18 or intergrin-2 (Workshop III). It complexes non-covalently with either L, M, or X integrin (CD11a, b, or c) to form the heterodimers. LFA-1, MAC-1, and p150,95, respectively. LFA-1 is the receptor for three members of the Ig supergene family of proteins, ICAM-1 (CD54, ICCAM-2 (CD102), and Mac-1 and p150,95 bind to ICAM-1, fibrinogen, and IC3b. ICAM-3 (CD50). CD18/CD11 heterodimeric molecules are involved with cell/cell and cell/extracellular adhesion in immune and inflammatory responses. This Mab blocks these cellular interactions. 68-5A5 was clustered at the IIIrd International Workshop on human leucocyte differentiation antigens.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
68-5A5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
McMichael A.J.et al., Leucocyte typing III, Oxford University Press, Oxford, (1987)
Insulin is a protein consisting of an ?-chain of 21 amino acids and a ?-chain of 30 amino acids and produced in the ?-cells of the pancreas. 2D11-H5 is a specific insulin antibody as tested by ELISA and on human pancreatic tissue.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2D11-H5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
De la Tour, D, et al, Mol. Endoc. 15: 476-483 (2001)
References 2:
Rajagopal, J, et al, Science 299: 363 (2003)
References 3:
Morisset, J, et al, J. Histochem. Cytochem. 51: 1501-1513 (2003)
The antibody reacts specificly with Human IL-1RII. The IL-1 system includes two agonists (IL-1alpha and IL-1beta), converting enzymes, antagonists, two receptors (IL-1RI and IL-1RII) and the IL-1 receptor accessory protein. The IL-1RII is part of the antagonistic IL-1 mechanism. It is also known as decoy receptor and is a non signaling molecule which functions by capturing IL-1 and preventing it from interacting with the signalling IL-1RI. The decoy IL-1RII can after binding to IL-1 also recruit the IL-1 receptor accessory protein and thus inhibit by coreceptor competition. Further a soluble form of IL-1RII exists which is shed, a process in which matrix metalloproteases have been found to play a role, by various cells including monocytes, polymorphonuclear cells, B cells and fibroblasts.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
6G5
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Mantovani; A et al. Ann N Y Acad Sci 1998; 840: 338
The antibody reacts specificly with Human IL-1RII. The IL-1 system includes two agonists (IL-1alpha and IL-1beta), converting enzymes, antagonists, two receptors (IL-1RI and IL-1RII) and the IL-1 receptor accessory protein. The IL-1RII is part of the antagonistic IL-1 mechanism. It is also known as decoy receptor and is a non signaling molecule which functions by capturing IL-1 and preventing it from interacting with the signalling IL-1RI. The decoy IL-1RII can after binding to IL-1 also recruit the IL-1 receptor accessory protein and thus inhibit by coreceptor competition. Further a soluble form of IL-1RII exists which is shed, a process in which matrix metalloproteases have been found to play a role, by various cells including monocytes, polymorphonuclear cells, B cells and fibroblasts.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
8.5
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Mantovani; A et al. Ann N Y Acad Sci 1998; 840: 338
PN-15 reacts with a lectin receptor like glycoprotein of 200 kDa (gp200), present in proximal renal tubules and on urothelium. The antigen is carbohydrate in nature. Other normal tissues that display the antigen include breast, parathyroid glands, thymus and epididymis. Among renal carcinomas 93% of primary and 84% of metastatic carcinomas are positive. Bladder cancers are also largely positive. Other tumor types include breast cancer, teratocarcinomas and parathyroid adenomas. The antigen, also called DEC-205, was assigned to CD205 at CD workshop VII. In the immune system it can facilitate tolerance to self-antigens through uptake of apoptosis derived material by dendritic cells, which in turn present fragments through MHC II and MHC I, either inducing or repressing immune responses, depending on the nature of concomitant signals.
Antibody Isotype:
IgG2b-kappa
Monosan Range:
MONOSAN
Clone:
PN-15
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Yoshida, S.O. et al, Cancer Res 49: 1802-1809 (1989)
References 2:
Li, G, et al, Anticancer Res. 20(4): 2773-8 (2000)
References 3:
Batchelder C.A. et al, Anat Rec (Hoboken) 297(8): 1392-1406 (2014)
References 4:
Cykowski M.D. et al, Ultrastruct Pathol 39(1): 69-77 (2015)
Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called âpathogen-associated molecular patternsâ. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFκB-dependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single ?-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses. Toll-like receptor 4 (TLR4; CD284) has been identified, next to MD-2 and CD14, as a receptor that is central to the innate immune response to LPS of Gram-negative bacteria. TLR4 is unique among TLRs in its ability to activate two distinct signaling pathways; one pathway is activated by the adaptors TIRAP (Toll/interleukin-1- receptor (TIR)-domain-containing adaptor protein) and MyD88, which leads to the induction of proâinflammatory cytokines. The second pathway is activated by the adaptors TRIF (TIR-domaincontaining adaptor protein inducing interferonâβ) and TRAM (TRIFrelated adaptor molecule), which leads to the induction of type I interferons. The monoclonal antibody HTA125 is a TLR4 function-blocking antibody. HTA125 recognizes preferentially human TLR4 that is associated with MD-2.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
HTA125
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Shimazu; R et al. J Exp Med 1999; 189: 1777
References 2:
Tabeta, K et al Infect Immun 2000, 68: 3731
References 3:
Akashi; S et al. Biochem Biophys Res Commun 2000; 268: 172
The monoclonal antibody T2.5 recognizes human Toll-like receptor 2 (TLR2). Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. At present, ligands for several of the TLR's, such as TLR2-6,9, have been identified, confirming their role in first line defense against invading microorganism. In mammals, TLRs are identified as type I transmembrane signaling receptors with an extracellular portion containing leucine-rich repeats with pattern recognition capabilities. Pathogen recognition by TLRs provokes rapid activation of innate immunity by inducing proliferation of proinflammatory cytokines and upregulation of costimulatory molecules and eventually toinitiation of adaptive immunity. TLR2 has been identified as a receptor that is central to the innate immune response to lipoproteins of Gram-negative bacteria, several whole Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. It is suggested that TLR2 is able to recognize such a wide variety of PAMPs (pathogen-specific molecular patterns) by forming heterodimers with other TLRs like e.g. TLR6. TLR2 is essential for recognizing lipopeptides and lipoproteins from several microorganisms and also peptidoglycans derived from gram-positive bacteria. Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
T2.5
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Meng; G et al. J Clin Invest 2004; 113: 1473
References 2:
Roura-Mir, C et al J Immunol 2005, 175: 1758
References 3:
Leemans; J et al. J Clin Invest 2005 115: 2894
References 4:
Spiller S et al. JEM 2008; 205: 1747
References 5:
Sutmuller R et al. J Clin Invest 2006; 116:485
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