YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP). YFP has an excitation peak at 514 nm and emission peak at 527 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
C-YFP tagged proteins from Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal of YFP protein. This peptide is conserved in pGWB541 Vector.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Li et al. (2021) Two ubiquitin-associated ER proteins interact with COPT copper transporters and modulate their accumulation, Plant Physiology, 2021;, kiab381, https://doi.org/10.1093/plphys/kiab381Lung et al. (2021) Oxylipin signaling in salt-stressed soybean is modulated by ligand-dependent interaction of Class II acyl-CoA-binding proteins with lipoxygenase. Plant Cell. 2021 Dec 17:koab306. doi: 10.1093/plcell/koab306. Epub ahead of print. PMID: 34919703.
Sub1C is a protein which is involved in rice tolerance to submergence.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Oryza sativa
Expected Species:
Oryza sativa
Immunogen:
KLH-conjugated synthetic peptide derived from Oryza sativa Sub1C. Chosen peptide is convered in all 6 isoforms (C-1 to C-6).
Homeobox protein SHOOT MERISTEMLESS is a protein required for shoot apical meristem (SAM) formation during embryogenesis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus
Immunogen:
KLH-conjugated peptide chosen from Arabidopsis thaliana STM protein sequence, UniProt: Q38874,TAIR: At1g62360
WUSCHEL is a transcription factor which plays a central role during early embryogenesis, oogenesis, flowering.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana WUSCHEL protein sequence, UniProt: Q9SB92, TAIR:At2g17950
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Dory et al. (2016). Kinase-Associated Phosphoisoform Assay: a novel candidate-based method to detect specific kinase-substrate phosphorylation interactions in vivo. BMC Plant Biol. 2016 Sep 21;16(1):204.
Ferredoxins are soluble, iron-sulfur containg proteins that function as electron donors in many metabolic pathways. Ferredoxin is also a target for thioredoxin action.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Terrauchi et al. (2009). Pattern of expression and substrate specificity of chloroplast ferredoxins from Chlamydomonas reinhardtii. J Biol Chem 284 (38):25867-25878.
Special application note:
This product can be sold containing ProClin if requested
Protease Do-like 7 (DegP7) EC=3.4.21 is a serine/cysteine protease involved in photoiunhibition process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica oleracea
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana DegP7 UniProt: Q8RY22-1 , TAIR: At3g03380
Antibody will provide a good signal on enriched nuclei fractions but not on total cell extracts
Application Details:
1 : 500 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
119,8 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Schuhmann et al. (2011). A new principle of oligomerization of plant DEG7 protease based on interactions of degenerated protease domains. Biochem. J. 435:167-174.
AAA2 domain is present in ATP binding proteins involved in stress response and being members of clpA/clpB family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
DHAR2 ( Dehydroascorbate Reductase 2) the protein is induced by jasmonic acid and oxidative chemical stresses and is a key component of the ascorbate recycling system. Involved in redox homeostasis under biotic and abiotic inducers. Localized in cytoplasm. Synonymes: chloride intracellular channel homolog 2, CLIC homolog 2, glutathione-dependent dehydroascorbate reductase 2, DHAR2, CytDHAR, GSH-dependent dehydroascorbate reductase 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Ricinus communis, Populus trichocarpa Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known DHAR1 sequence of Arabidopsis thaliana Q9FRL8, At1g75270
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Grefen et al. (2009). The determination of protein-protein interactions by the mating-based split-ubiquitin system (mbSUS). Methods Mol Biol 479:217-233.
DHAR1 ( Dehydroascorbate Reductase 1) the protein is induced by jasmonic acid and oxidative chemical stresses and is a key component of the ascorbate recycling system. Involved in redox homeostasis under biotic and abiotic inducers. Localized in mitochondria. Synonymes: glutathione-dependent dehydroascorbate reductase 1, chloride intracellular channel homolog 1, CLIC homolog 1, glutathione-dependent dehydroascorbate reductase 1, AtDHAR1, GSH-dependent dehydroascorbate reductase 1, mtDHAR, AT1G19570.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Nicotiana tabacum, Populus trichocarpa, Ricinus communis, Solanum tuberosum, Triticum aestivum, Zinnia elegans Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known DHAR1 sequence of Arabidopsis thaliana Q9FWR4, At1g19570
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Szymańska et al. (2019). SNF1-Related Protein Kinases SnRK2.4 and SnRK2.10 Modulate ROS Homeostasis in Plant Response to Salt Stress. Int J Mol Sci. 2019 Jan 2;20(1). pii: E143. doi: 10.3390/ijms20010143.Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008.Wang et al. (2014). Proteomic analysis of salt-responsive proteins in the leaves of mangrove Kandelia candel during short-term stress. PLoS One. 2014 Jan 8;9(1):e83141. doi: 10.1371/journal.pone.0083141. eCollection 2014.
ADP-glucose pyrophosphorylase (AGPase) catalyses the first committed step in the synthesis of transient starch in chloroplasts and storage starch in amyloplasts in plants (Tetlow et al., 2004). AGPase in higher plants is composed of two distinct subunits encoded by separate genes, forming an L2S2 heterotetramer. The large subunits (L) are modulators of allosteric activity, while the small subunits (S) are the catalytic subunits (Kim et al., 2007). Synonymes:ADP-glucose pyrophosphorylase, ADP-glucose synthase, AGPase B, Alpha-D-glucose-1-phosphate adenyl transferase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cross reactivity of this antibody to Rubisco has been excluded using 2D gel electrophoresis.Antigen used to elicit this antbody has very poor conservation in large subunit of ADP-glucose pyrophosphorylase.
Application Details:
1 : 1000-1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
49,4 | 52 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Lim et al (2022). Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Ma et al. (2021) A plasma membrane transporter coordinates phosphate reallocation and grain filling in cereals. Nat Genet. 2021 Apr 29. doi: 10.1038/s41588-021-00855-6. Epub ahead of print. PMID: 33927398.Chang et al. (2020). Enhanced lipid productivity in AGP knockout marine microalga Tetraselmis sp. using a DNA-free CRISPR-Cas9 RNP method. Bioresource Technology.Volume 303, May 2020, 122932. Ancin et al. (2019). NTRC and Thioredoxin f Overexpression Differentially Induces Starch Accumulation in Tobacco Leaves.Plants (Basel). 2019 Nov 26;8(12). pii: E543. doi: 10.3390/plants8120543.Takahashi et al. (2019). Glutelin subtype-dependent protein localization in rice grain evidenced by immunodetection analyses. Plant Mol Biol. 2019 Mar 25. doi: 10.1007/s11103-019-00855-5.
EF1A (elongation factor 1-alpha) belongs to the GTP-binding elongation factor family and is localized in the cytoplasm. It is an essential enzyme in elongation phase of protein synthesis. There are four genes encoding EF1A in Arabidopsis, sequences of genes 1,2 and 3 are identical. Synonymes:eEF-1A.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Brassica napus, Nicotiana benthamiana, Picea sitchensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
Full length, recombinant EF1A of Arabidopsis thaliana UniProt: P0DH99-1, TAIR: AT1G07940
Markovi? et al. (2021)Correlation of elongation factor 1A accumulation with photosynthetic pigment content and yield in winter wheat varieties under heat stress conditions,Plant Physiology and Biochemistry,Volume 166,2021,Pages 572-581, ISSN 0981-9428,https://doi.org/10.1016/j.plaphy.2021.06.035.(https://www.sciencedirect.com/science/article/pii/S098194282100348X)Djuki? et al. (2019). Resolving subcellular plant metabolism. Plant J. 2019 Jul 30. doi: 10.1111/tpj.14472.Zhen et al. (2018). 2D-DIGE comparative proteomic analysis of developing wheat grains under high-nitrogen fertilization revealed key differentially accumulated proteins that promote storage protein and starch biosyntheses. Anal Bioanal Chem. 2018 Jul 30. doi: 10.1007/s00216-018-1230-4.Wang et al. (2016). GOLGI TRANSPORT 1B Regulates Protein Export from the Endoplasmic Reticulum in Rice Endosperm Cells. Plant Cell. 2016 Nov;28(11):2850-2865. (Oryza sativa, western blot)
GAI is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Less sensitive to GA. Synonymes:GRAS family protein 3, AtGRAS-3, Gibberelic acid-insensitive mutant protein, Restoration of growth on ammonia protein 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conugated synthetic peptide, chosen from Arabidopsis thaliana GAI protein sequence UniProt: Q9LQT8, TAIR: At1g14920
Homogenization with thiourea and bead beater. Protocol for protein extraction from seeds can be requested here. GAI protein is present in very low levels therefore specific material should be used for analysis as well as chemiluminescence detection reagent in extreme low femtogram range, as AgriseraECLSuperBright.
Application Details:
1 : 1000 (WB) on transfected Arabidopsis thaliana protoplasts
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 25 l of sterile water
Molecular Weight:
58,9 kDa
Not reactive in:
Brassica napus, Populus sp., Solanum lycopersicum
Selected references:
Gorshkova & Pojidaeva (2021). Members of the Universal Stress Protein Family are Indirectly Involved in Gibberellin-Dependent Regulation of Germination and Post-Germination Growth. Russ J Plant Physiol 68, 451 ??462 (2021). https://doi.org/10.1134/S1021443721030055Shahnejat-Bushehri et al. (2016). Arabidopsis NAC transcription factor JUB1 regulates GA/BR metabolism and signalling. NATURE PLANTS 2: Article number: 16013, 2016.
DELLA protein RGA is a putative transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. It is a member of VHIID/DELLA regulatory family and is the most sensitive to GA application, compare to other DELLA proteins.Involved in fruit and flower development. Synonymes: GAI-related sequence, GRAS family protein 10, AtGRAS-10, Repressor on the ga1-3 mutant, Restoration of growth on ammonia protein 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide chosen from RGA of Arabidopsis thaliana UniProt: Q9SLH3,TAIR: At2g01570
RGA protein is prone to degradation therefore caution has to be taken during protein extraction
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
64 | 64 kDa
Not reactive in:
Brassica napus, Populus sp., Rosa chinensis, Triticum aestivum
Selected references:
Dong et al. (2021). An HB40 - Jungbrunnen1 - GA 2-OXIDASE regulatory module for gibberellin homeostasis in ArabidopsisYan et al. (2020). FKF1 F-box Protein Promotes Flowering in Part by Negatively Regulating DELLA Protein Stability Under Long-Day Photoperiod in Arabidopsis. J Integr Plant Biol . 2020 May 19. doi: 10.1111/jipb.12971.Ferrero et al. (2019). Class I TCP transcription factors target the gibberellin biosynthesis gene GA20ox1 and the growth promoting genes HBI1 and PRE6 during thermomorphogenic growth in Arabidopsis. Plant Cell Physiol. 2019 Jul 11. pii: pcz137. doi: 10.1093/pcp/pcz137.Lorrai et al. (2018). Abscisic acid inhibits hypocotyl elongation acting on gibberellins, DELLA proteins and auxin. AoB Plants. 2018 Oct 5;10(5):ply061. doi: 10.1093/aobpla/ply061.Chahtane et al. (2018). The plant pathogen Pseudomonas aeruginosa triggers a DELLA-dependent seed germination arrest in Arabidopsis. Elife. 2018 Aug 28;7. pii: e37082. doi: 10.7554/eLife.37082.
HSP90-2 (heat shock protein 90-2) is a constitutive isoform of HSP90, expressed in all tissues and abundant in root apical meristem, pollen and tapetum. Alternative names: ATHSP90.2, EARLY-RESPONSIVE TO DEHYDRATION 8, ERD8, HEAT SHOCK PROTEIN 90.2, HEAT SHOCK PROTEIN 81-2, HEAT SHOCK PROTEIN 81.2, HEAT SHOCK PROTEIN 90.2, HSP81-2, HSP81.2, HSP90.2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Sorghum bicolor
Expected Species:
Glycne max, Hordeum vulgare, Micromonas pulsilla, Nicotiana benthamina, Nicotiana tabacum, Ostreococcus lucimarinus, Oryza sativa, Physcomitrium patens, Populus balsamifera, Ricinus communis, Solanum tuberosum, Triticum aestivum, Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
Full length recombinant HSP90-2 of Arabidopsis thaliana, UniProt: F4K6B6-1, TAIR: AT5G56030
This product can be sold containing ProClin if requested
Application Details:
1 : 3000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
80.6 | 95 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Gao et al. (2021) Identification of a bacterial-type ATP-binding cassette transporter implicated in aluminum tolerance in sweet sorghum (Sorghum bicolor L.). Plant Signal Behav. 2021 Jul 3;16(7):1916211. doi: 10.1080/15592324.2021.1916211. Epub 2021 May 26. PMID: 34034635; PMCID: PMC8205057.Barghetti et al. (2017). Heat-shock protein 40 is the key farnesylation target in meristem size control, abscisic acid signaling, and drought resistance. Genes Dev. 2017 Nov 15;31(22):2282-2295. doi: 10.1101/gad.301242.117.He et a. (2012).SpecificMissenseAlleles of theArabidopsisJasmonicAcidCo-ReceptorCOI1RegulateInnateImmuneReceptorAccumulation andFunction. PloS Genetics, Open Access.
Special application note:
Antibody reacts more with constitutive isofrom Hsp90-2 than heat inducible Hsp90-1
HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family and is involved in protein complex oligomerization and protein folding. Alternative names: AtHsp18.5, 18.5 kDa heat shock protein.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare
Expected Species:
Glycne max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, Zea maysSpecies of your interest not listed? Contact us
Hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38 C for 3 hours at high humidity.Please, note that longer transfer time might result in losing a signal for this protein.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
18,5 kDa
Not reactive in:
Prosopis cineraria
Selected references:
Sadura et al. (2020). HSP Transcript and Protein Accumulation in Brassinosteroid Barley Mutants Acclimated to Low and High Temperatures . Int J Mol Sci . 2020 Mar 10;21(5):1889.doi: 10.3390/ijms21051889.
Special application note:
As hsp18,5 is a low abundancy protein, please, make sure that the plants are heated to the right temperature, Normally I heat stress the seedling on a sealed agar plate for 2 hours, This assures that the humidity around the plant is very high, Low humidity can allow the plant to cool down through transpiration, If a plant is in soil you can keep the leaf or even a whole plant over a wet filter paper and seal the plate very well during the treatment, Heat stressing plants in microphage tubes does not work that well
The retinoblastoma protein Rb is considered to be a key regulator of G1/S phase transition by blocking S phase entry and cell growth. Plant retinoblastoma-related (RBR) proteins share a homology with the human tumour suppressor retinoblastoma (pRb) protein. RBR protein functions are controlled by phosphorylation and protein-protein interactions. Short name: AtRBR
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C. Upon arrival Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Arabidopsis thaliana, Medicago sativa
Expected Species:
Camelia sinensis, Chenopodium rubrum, Cocos nucifera, Hordeum vulgare, Oryza sativa, Pisum sativum, Populus tremula, Scutelaria baicalensis, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
Recombinant C-terminal fragment consisting of 236 amino acids of Arabidopsis thaliana retinoblastoma protein UniProt: Q9LKZ3, lTAIR: At3g12280
Applications:
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western blot (WB)
This antibody is not suitable for immunolocalization.Methanol concentration in a transfer buffer can be considerably reduced or for a better transfer of high MW proteins (even with PVDF membrane).For immunoprecipitation start with 2 l and titrate it depending upon your experimental conditions. Please note that you work with a total IgY fraction, which means that it will contain between 40-60 g of total IgY (directed not only against retinoblastoma) therefore all of this IgY needs to be captured by the anti-IgY matrix.As control pre-serum for IP this product can be used, total, pre-immune IgY.
Application Details:
2 l (IP), 1 : 2000 (WB)
Purity:
Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
Molecular Weight:
112 kDa
Not reactive in:
Chlamydomonas reinhardtii
Selected references:
Leviczky et al. (2019). E2FA and E2FB transcription factors coordinate cell proliferation with seed maturation. Development. 2019 Nov 26;146(22). pii: dev179333. doi: 10.1242/dev.179333.Horvath et al. (2017). Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control. EMBO J. 2017 May 2;36(9):1261-1278. doi: 10.15252/embj.201694561. Epub 2017 Mar 20.Cheng et al. (2013). Down-regulation of multiple CDK inhibitor ICK/KRP genes up-regulates E2F pathway and increases cell proliferation, organ and seed sizes in Arabidopsis. Plant j. May 7. brah m et al. (2011). Immunodetection of retinoblastoma-related protein and its phosphorylated form in interphase and mitotic alfalfa cells. J Exp Bot 62(6):2155-2168.
Donkey anti-rat IgG (H&L), is a secondary HRP (horse radish peroxidase) conjugated antibody which binds to rat IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Bui et al. (2020). Differential submergence tolerance between juvenile and adult Arabidopsis plants involves the ANAC017 transcription factor. doi.org/10.1101/2020.02.12.945923 BioRxiv.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins by immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
PsaC is a conserved, chloroplast-encoded, Fe-S binding protein of approximately 10kDa, present in all known Photosystem I complexes. It is located on the stromal side of the thylacoid membranes. PsaC coordinates the Fe–S clusters FA and FB through two cysteine-rich domains.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
In some species minor cross reactions with some larger proteins are seen. These may contain related iron-sulfur binding motifs. Therefore size verification of the reacting band is required. Due to the small size of the protein, care should be taken to differentiate between chemiluminescent signal from PsaC and non-specific signals from chlotophylls or lipids if pigment is retained near the bottom of the blot.For the most optimal results use:thylakoid membranes or PSI particles, solubilized in a SDS sample buffer (final concentrations: 63 mM Tris HCl, 10% glycerol, 2% SDS, 0.0025% bromophenol blue) with 2.5% beta-mercaptoethanol at 85C for 2 minutes. The samples were spun softly, then the supernatant loaded. This product can be sold containing ProClin if requested.
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
9 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9Ye et al. (2022) Effect of increased CO2 on iron-light-CO2 co-limitation of growth in a marine diatom, ASLO, Limnol. Oceanogr. 2022, 172-176Rogowski et al. (2021) Light as a substrate: migration of LHCII antennas in extended Michaelis-Menten model for PSI kinetics. J Photochem Photobiol B. 2021 Dec;225:112336. doi: 10.1016/j.jphotobiol.2021.112336. Epub 2021 Oct 19. PMID: 34736069.Levitan et al. (2019). Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17316-17322. doi: 10.1073/pnas.1906726116.Zavrel et al. (2019). Quantitative insights into the cyanobacterial cell economy. Elife. 2019 Feb 4;8. pii: e42508. doi: 10.7554/eLife.42508.
Special application note:
Peptide target used to elicit this antibody is well conserved in all photoautotrophs except some cyanobacteria, some red algae and Cyanophora paradoxa, which contain a conserved substitution of a valine to an isoleucine. The performance of the antibodies has been confirmed against taxa containing both the valine and isoleucine variants.Example of a simulataneous western blot detection with RbcL, PsbA and PsaC antibodies. More information about quantitative western blot using PsaC antibody can be found here.
COX11 (cytochrome c oxidase assembly protein) , involved in respiratory chain complex IV assembly.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardii
Expected Species:
Physcomitrium patensSpecies of your interest not listed? Contact us
Immunogen:
Recombinant fragment of Chlamydomonas reinhardtii COX11 UniProt: A8JCS0
CGL78 is a protein conserved in the green lineage and predicted to be located in the chloroplast. Member of the Ycf54 superfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Please, omitt SDS from transfer buffer and reduce transfer time to 45 min, Nitrocellulose membrane is recommended and SDS is omitted to allow this LMW protein to bind tighter to the membrane
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
24 kDa
Not reactive in:
Hordeum vulgare
Selected references:
Hsieh et al. (2013). The Proteome of Copper, Iron, Zinc, and Manganese Micronutrient Deficiency in Chlamydomonas reinhardtii. Mol Cell Proteomics. 2013 Jan;12(1):65-86. doi: 10.1074/mcp.M112.021840. Epub 2012 Oct 13.
Special application note:
Currently this antibody has not been confirmed to detect CGL78 protein in Arabidopsis thaliana. If you are interested to use this antibody in Arabidopsis, please, contact us.
EF1A (elongation factor 1-alpha) belongs to the GTP-binding elongation factor family and is localized in the cytoplasm. It is an essential enzyme in elongation phase of protein synthesis. There are four genes encoding EF1A in Arabidopsis, sequences of genes 1,2 and 3 are identical. Synonymes:eEF-1A.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This product can be sold containing ProClin if requested.Following protein resolubilization with urea/thiourea/CHAPS signal strength of recognized band is decreased. Recommended protein extraction conditions are without urea.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
49,5 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Pan et al. (2021) Post-Golgi Trafficking of Rice Storage Proteins Requires the Small GTPase Rab7 Activation Complex MON1-CCZ1, Plant Physiology, 2021;, kiab175, https://doi.org/10.1093/plphys/kiab175Ren et al. (2020). GPA5 Encodes a Rab5a Effector Required for Post-Golgi Trafficking of Rice Storage Proteins. Plant Cell. 2020 Jan 16. pii: tpc.00863.2019. doi: 10.1105/tpc.19.00863.Djuki? et al. (2019). Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress. Journal of Plant Physiology Volume 240, September 2019, 153015.Foley et al. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate.Dev Cell. 2017 Apr 24;41(2):204-220.e5. doi: 10.1016/j.devcel.2017.03.018.
AKIN11 (E.C.= 2.7.11.1) is a catalytic subunit of the putative trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. Synonymes: AKIN alpha-1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Gutierrez-Beltran et al. (2021) Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis. EMBO J. 2021 Jul 21:e105043. doi: 10.15252/embj.2020105043. Epub ahead of print. PMID: 34287990.Pedrotti et al. (2018). Snf1-RELATED KINASE1-Controlled C/S1-bZIP Signaling Activates Alternative Mitochondrial Metabolic Pathways to Ensure Plant Survival in Extended Darkness. Plant Cell. 2018 Feb;30(2):495-509. doi: 10.1105/tpc.17.00414. Epub 2018 Jan 18.Emanuelle et al. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant J. 2015 Mar 3. doi: 10.1111/tpj.12813.
Goat anti-rabbit IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG (H&L)
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Linster et al. (2015). Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis. Nat Commun. 2015 Jul 17;6:7640. doi: 10.1038/ncomms8640.
Special application note:
Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidasePurity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.
GDP-L-Galactose Phosphorylase is a histidine triad (HIT) enzyme of the Smirnoff-Wheeler pathway of ascorbic acid synthesis in plants. Encoded by VTC2 gene. The enzyme catalyzes the conversion of GDP-L-galactose to L-galactose 1-phosphate in a reaction that consumes inorganic phosphate and produces GDP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica oleracea var. italica, Brassica rapa var. komatsuna, Citrus limon, Nicotiana tabacum, Spinacia oleracea, Zea mays, Chlamydomonas reinhardtii
Expected Species:
Manihot esculenta, Sorghum bicolor, Oryza sativa, Physcomitrium patensSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjuated synthetic peptide derived from known GDP-L-Galactose Phosphorylase sequences, including Arabidopsis thaliana Q8LKQ7 (At4g26850) and Chlamydomonas reinhardtii
PHOT2 | phototropin-2 is a membrane-bound serine/threonine kinase that functions as blue light photoreceptor in redundancy with PHOT1. Involved in processed like stomatal opening, chloroplast movement and phototropism. Alternative names: NPL1, K21L19.6, AtKin7, Defective in chloroplast avoidance protein 1Non-phototropic hypocotyl 1-like protein 1, NPH1-like protein 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana PHOT2 P93025, At5g58140
Labuz et al. (2021) Phototropin interactions with SUMO proteins. Plant Cell Physiol. 2021 Feb 17:pcab027. doi: 10.1093/pcp/pcab027. Epub ahead of print. PMID: 33594440.Krzeszowiec et al. (2020). Chloroplasts in C3 grasses move in response to blue-light. Plant Cell Rep . 2020 Oct;39(10):1331-1343.doi: 10.1007/s00299-020-02567-3. Epub 2020 Jul 13.Labuz et al. (2015). The impact of temperature on blue light induced chloroplast movements in Arabidopsis thaliana. Plant Science, doi:10.1016/j.plantsci.2015.07.013.Aggarwal et al. (2014). Blue-light-activated phototropin2 trafficking from the cytoplasm to Golgi/post-Golgi vesicles. J Exp Bot. 2014 May 12.
Special application note:
This product can be sold containing ProClin if requested
UniProt number:
P93025
TAIR number:
AT5G58140
Research area:
Arabidopsis thaliana antibodies
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