Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Chicken
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Chicken
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Chicken
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Accession Number:
AAA51107
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins
Country Of Origin:
Chicken Ig fraction was prepared using serum from healthy hens of US origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
PBA1 (20S proteasome beta subunit A1) is a subunit of proteasome, multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Alternative name: Proteasome subunit beta.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Boussardon, Bag, Juvany, et al. (2022) The RPN12a proteasome subunit is essential for the multiple hormonal homeostasis controlling the progression of leaf senescence. Commun Biol. 2022;5(1):1043. Published 2022 Sep 30. doi:10.1038/s42003-022-03998-2Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.
Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. It occupies a key position both for transferring the photoreducing power to Fd-NADP+ oxidoreductase (FNR), hence the formation of NADPH, and for mediating the cyclic electron flow around photosystem I (PSI).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 1 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Brassica napus, Brassica oleracea, Eutrema salsugineum, Raphanus sativusSpecies of your interest not listed? Contact us
Immunogen:
Purified full length, tag cleaved, recombinant maize Fd1, UniProt: P27787
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
12 | 15 kDa
Not reactive in:
Nicotiana benthamiana
Selected references:
Hanke and Hase (2008). Variable Photosynthetic Roles of Two Leaf-Type Ferredoxins in Arabidopsis, as Revealed by RNA Interference. Photochem Photobiol. 84(6):1302-9. doi: 10.1111/j.1751-1097.2008.00411.x.Kimata and Hase (1989). Localization of Ferredoxin Isoproteins in Mesophyll and Bundle Sheath Cells in Maize Leaf. Plant Physiol. 89(4):1193-7. doi: 10.1104/pp.89.4.1193.
Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Fd3 is non-photosynthetic Fd expressed more in root than in leaf. Fd3 is localised in chloroplast and plastids.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 1 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Brachypodium distachyon, Dichanthelium oligosanthes, Hordeum vulgare, Oryza sativa, Panicum hallii, Saccharum sp. , Setaria italicaSpecies of your interest not listed? Contact us
Immunogen:
Purified full length, tag cleaved, recombinant maize Fd3, UniProt: P27788
Antibody reacts weakly with other ferredoxins: Arabidopsis thaliana and Zea mays Fd2 and Zea mays Fd6.
Application Details:
1: 2000 - 1: 10 000 (WB)
Purity:
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
16 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Hanke and Hase (2008). Variable Photosynthetic Roles of Two Leaf-Type Ferredoxins in Arabidopsis, as Revealed by RNA Interference. Photochem Photobiol. 84(6):1302-9. doi: 10.1111/j.1751-1097.2008.00411.x. Hanke et al. (2003). A Post Genomic Characterization of Arabidopsis Ferredoxins. Plant Physiol. 134(1):255-64. doi: 10.1104/pp.103.032755. Matsumura et al. (1997). A Nitrate-Inducible Ferredoxin in Maize Roots. Genomic Organization and Differential Expression of Two Nonphotosynthetic Ferredoxin Isoproteins. Plant Physiol. 114(2):653-60. doi: 10.1104/pp.114.2.653.
Goat anti-Mouse IgG (H&L), F(ab)'2 fragment, min. cross-reactivity to bovine, horse, human, pig or rabbit serum protein is a secondary antibody which binds to mouse IgG (H&L), F(ab)'2 Fragment in immunological assays.
For reconstitution add 0,55 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins • serum proteins from bovine, horse, human rabbit or swine .
Goat anti-Mouse IgG (H&L), F(ab)'2 Fragment, min. cross-reactivity to bovine, horse, human, pig or rabbit serum protein is a secondary antibody which binds to mouse IgG (H&L), F(ab)'2 Fragment in immunological assays.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins • serum proteins from bovine, horse, human rabbit or swine .
Goat anti-Mouse IgG (H&L), TRITC conjugated, min. cross-reactivity to bovine, goat, human, rabbit or rat IgG is a secondary antibody conjugated to TRITC, which binds to mouse IgG (H&L) in immunological assays
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • bovine, goat, human, rabbit or rat IgG Based on ELISA, this antibody has <1% reactivity to: • rat IgG .
Chicken anti-Mouse IgG (H&L), min. cross-reactivity to human or rabbit IgG/serum proteins is a secondary antibody which binds to mouse IgG (H&L) in immunological assays.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified chicken IgY.
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins • IgG/serum from human or rabbit .
Goat anti-Mouse IgG (H&L), HRP conjugated, min. cross-reactivity to human IgG or serum proteins is a secondary antibody conjugated to HRP, which binds to mouse IgG (H&L) in immunological assays.
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1% (v/v) Kathon CG is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins • human IgG or serum proteins .
Goat anti-Mouse IgG (H&L), HRP conjugated, min. cross-reactivity to bovine, goat, human, rabbit or rat IgG is a secondary antibody conjugated to HRP, which binds to mouse IgG (H&L) in immunological assays.
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1% (v/v) Kathon CG is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • bovine, goat, human, rabbit or rat IgG Based on ELISA, this antibody has <1% reactivity to: • rat IgG .
Goat anti-Mouse IgG (H&L), DyLight 680 conjugated is a secondary antibody conjugated to DyLight 680, which binds to mouse IgG (H&L) in immunological assays.DyLight 680 has Amax = 682 nm, Emax = 715 nm.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins .
Goat anti-Mouse IgG (H&L), DyLight 488 conjugated, min. cross-reactivity to bovine, horse, human, pig or rabbit serum proteins is a secondary antibody conjugated to DyLight 488, which binds to mouse IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins • serum proteins from bovine, horse, human rabbit or swine .
Goat anti-Mouse IgG (H&L), DyLight 405 conjugated is a secondary antibody conjugated to DyLight 405, which binds to mouse IgG (H&L) in immunological assays.DyLight 405 has Amax = 400 nm, Emax = 420 nm.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins .
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • bovine, goat, human, rabbit or rat IgG Based on ELISA, this antibody has <1% reactivity to: • rat IgG .
Donkey anti-Rabbit IgG (H&L) - Affinity Pure, DyLight 633 Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG is a secondary antibody conjugated to DyLight 633, which binds to rabbit IgG (H&L) in immunological assays.DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
immunolocalization Donkey anti-rabbit conjugated to DY Light 633 in the absence of primary antibody show very little background label (right image) and binds to primary antibodies (anti-Arf1 and anti-Sec21p; two left images). The top images show only the antibody label in red and bottom images show overlay of antibody label in red with DAPI in blue and DIC/Nomarski image in grey. The material has been prepared using whole mount immunolabelling procedure of 5 day old Arabidopsis thaliana seedlings (Fischer, U. et al. (2006). Curr. Biol. 16, 2143–2149). Antibody dilutions: rabbit anti-sec21p (Agrisera, AS08 327) 1:1000, rabbit anti-Arf1 (Agrisera, AS08 325) 1:1000 and Agrisera Donkey anti-rabbit DY Light 633 1:100. DNA was stained with DAPI. Courtesy: Dr. Anna Gustavsson and Prof. Markus Grebe, Ume Plant Science Centre
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, no reactivity is observed to:non-immunoglobulin rabbit serum proteinsIgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat.Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.
UCP (uncoupling protein) is an inner membrane mitochondrial protein that can dissipate the proton gradien before it can be used to provide the energy for oxidative phosphorylation. Synonymes: AtUCP, Uncoupling protein 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Czobor et al. (2019). Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst. PLoS One. 2019 Jan 10;14(1):e0210592. doi: 10.1371/journal.pone.0210592.Tak č et al. (2018). Shot-Gun Proteomic Analysis on Roots of Arabidopsis pldα1 Mutants Suggesting the Involvement of PLDα1 in Mitochondrial Protein Import, Vesicular Trafficking and Glucosinolate Biosynthesis. Int J Mol Sci. 2018 Dec 26;20(1). pii: E82. doi: 10.3390/ijms20010082. (immunolocalization)Garmash et al. (2017). Expression profiles of genes for mitochondrial respiratory energy-dissipating systems and antioxidant enzymes in wheat leaves during de-etiolation. J Plant Physiol. 2017 Aug;215:110-121. doi: 10.1016/j.jplph.2017.05.023.Florez-Sarasa et al. (2016). Impaired cyclic electron flow around Photosystem I disturbs high-light respiratory metabolism. Plant Physiol. 2016 Oct 19. pii: pp.01025.2016.Liu et al. (2015). Silencing of mitochondrial uncoupling protein gene aggravates chilling stress by altering mitochondrial respiration and electron transport in tomato. Acta Physiologiae Plantarum November 2015, 37:223.Long et al. (2015). Contributions of photosynthetic and non-photosynthetic cell types to leaf respiration in Vicia faba L. and their responses to growth temperature. Plant Cell Environ. 2015 Apr 1. doi: 10.1111/pce.12544.Grabelnych et al. (2014). Mitochondrial Energy Dissipating Systems (Alternative Oxidase, Uncoupling Proteins, and External NADH Dehydrogenase) Are Involved in Development of Frost Resistance of Winter Wheat Seedlings. ISSN 0006 2979, Biochemistry (Moscow), 2014, Vol. 79, No. 6, pp. 506 519. Pleiades Publishing, Ltd., 2014.Barreto et al. (2014). Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response. BMC Plant Biol. 2014 May 28;14(1):144.
Special application note:
Peptide used to elicit this antibody is conserved in both isoforms, UCP1 and UCP2 of Arabidopsis thaliana.
Cytochrome c is located in inner mitochondrial membrane. It is a small heme protein which, unlike other cytochromes, is highly soluble. This protein is an essential component of the electron transport chain, where it undergoes oxidation and reduction without binding oxygen.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles and Store at -80°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
cytc1 and cytc2 from following species: A. theoprasi, Brassica napus, Brassica oleracea, Cannabis sativa, C. maxima, Chlamydomonas reinhardtii (peptide target partially conserved), Lupinus luteus, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Ostreococcus (peptide target partially conserved), P. aurea, Physcomitrium patens, Ricinus communis, S. nigra, Solanum lycopersivum, Vitis vinifera.Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana cytochrome c protein sequence, UniProt:D7KMK0 (C-1) D7LY03 (C-2), TAIR: At1g22840 (Cytc1) and At4g10040 (Cytc2)
The presence of cytochrome c in the cysotol is a marker of PCD (programmed cell death)
Application Details:
1: 100 (IL), 1 : 5000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
12.5 | 14 kDa (for Arabidopsis thaliana)
Not reactive in:
Arabidopsis thaliana CytC6, Chinesecuscuta sp.
Selected references:
Guo et al. (2021) The pentatricopeptide repeat protein GEND1 is required for root development and high temperature tolerance in Arabidopsis thaliana,Biochemical and Biophysical Research Communications,Volume 578,2021,Pages 63-69,ISSN 0006-291X,https://doi.org/10.1016/j.bbrc.2021.09.022.(https://www.sciencedirect.com/science/article/pii/S0006291X21013164)Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .Dai et al. (2020). Pentatricopeptide repeat protein DEK46 is required for multi-sites mitochondrial RNA editing and maize seed development. J Exp Bot. 2020 Jul 25;eraa348.doi: 10.1093/jxb/eraa348. Waltz et al. (2019). Small is big in Arabidopsis mitochondrial ribosome. Nat Plants. 2019 Jan;5(1):106-117. doi: 10.1038/s41477-018-0339-y.Doronina et al. (2019). Structural and Functional Features of the Wheat Embryo Sac’s Antipodal Cells during Differentiation. Russ J Dev Biol 50, 194–208. (immunolocalization)
Cytokeratin 20 (CK20) is expressed in enterocytes and goblet cells of the gastrointestinal (GI) tract. It is also expressed in specific types of simple epithelial cells of the urinary tract. CK20 is useful marker of colorectal carcinoma, gastric, pancreas, urothelium, merkel and biliary system carcinomas.
Cytokeratin 20 (CK20) is expressed in enterocytes and goblet cells of the gastrointestinal (GI) tract. It is also expressed in specific types of simple epithelial cells of the urinary tract. CK20 is useful marker of colorectal carcinoma, gastric, pancreas, urothelium, merkel and biliary system carcinomas.
Rabbit anti-Growth Associated Protein 43 (GAP-43) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
GAP43 is very abundant protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve (1). Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 were independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Rabbit
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
C-terminal peptide 217-227 of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB). A dilution of 1:5,000 - 1:20,000 is recommended. A dilution of 1:500-2,000 is recommended for Immunocytochemistry (ICC). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a 43 kDa band by Western blot on bovine cerebellum homogenate. The molecular weight of the protein recognized can vary (~43-57 kDa) depending on the species and the percentage acrylamide used in the SDS-PAGE gel. It has also been used successfully for immunocytochemistry.
Storage:
Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Tyrosine Kinase Receptor C (TrkC) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
TrkC is a member of the neurotrophic tyrosine receptor kinase family. TrkC is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC is the receptor for neurotrophin-3 (NT-3). Signalling through TrkC leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. Additional isoforms seem to exist. Mutations in TrkC have been associated with medulloblastomas, secretory breast carcinomas and other cancers.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Extracellular domain of glycosylated human TrkC protein produced in CHO cells was used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1:3000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity was demonstrated by immunohistochemistry. This antibody was used to stain cryostat sections of the rat peripheral sensory ganglia. Reacts with human, rat and mouse TrkC. Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
The PD-L1-TEC [IHC451] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC451
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Lung Adenocarcinoma
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Cookies:
X
We use cookies to help personalise and improve your web experience.
By using our website you consent to our use of cookies, some of which may have already been set on your device.
View our Cookie Policy to learn more.
