Iron-hydrogenase HydA2 is catalyzing reversible oxidation of molecular hydrogen. In Chlamydomonas the protein is present in low levels of 1 g/liter of culture.Synonyme: HYD1/1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Ostreococcus sp.Species of your interest not listed? Contact us
Immunogen:
Recombinant, full length Chlamydomonas reinhardtii HydA-2 Q8VZZ0
HydA1 (497aa) has a calculated MW of 53.1 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP has a calculated MW of 47.5 kDa and runs according to its size at about 48 kDa.HydA2 (505aa) has a calculated MW of 53.7 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP can only be estimated, since the cleavage site is known only from in silico analysis. It has a calculated MW of 47.3 kDa and should run in the gel also according to its size.This antibody is binding recombinant HydA1/2 protein.
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
53,7 | 48 kDa (after transit peptide is cleaved)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Jokel et al. (2020). Elimination of the flavodiiron electron sink facilitates long-term H2 photoproduction in green algae. Biotechnol Biofuels. 2019 Dec 5;12:280. doi: 10.1186/s13068-019-1618-1.Lindblad et al. (2019). CyanoFactory, a European consortium to develop technologies needed to advance cyanobacteria as chassis for production of chemicals and fuels. Algal Research, Volume 41, August 2019, 101510.Weiner at al (2018), Overcoming the expression barrier of the ferredoxin‑hydrogenase chimera in Chlamydomonas reinhardtii supports a linear increment in photosynthetic hydrogen output. Algal Research Volume 33, July 2018, Pages 310-315Wei et al. (2017). Light Intensity is Important for Hydrogen Production in NaHSO3-Treated Chlamydomonas reinhardtii. Plant Cell Physiol. 2017 Mar 1;58(3):451-457. doi: 10.1093/pcp/pcw216.Eilenberg et al. (2016). The dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux towards hydrogen production and elevated oxygen tolerance. Biotechnol Biofuels. 2016 Aug 30;9(1):182. doi: 10.1186/s13068-016-0601-3. eCollection 2016.Liran et al. (2016). Microoxic Niches within the Thylakoid Stroma of Air-Grown Chlamydomonas reinhardtii Protect [FeFe]-Hydrogenase and Support Hydrogen Production under Fully Aerobic Environment. Plant Physiol. 2016 Sep;172(1):264-71. doi: 10.1104/pp.16.01063. Epub 2016 Jul 21.Reifschneider-Wegner et al. (2014). Expression of the [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii. Int J of Hydrogen Energy, Volume 39, Issue 8, 6 March 2014, Pages 3657–3665.Pinto et al. (2013). Rubisco mutants of Chlamydomonas reinhardtii enhance photosynthetic hydrogen production. Appl Microbiol Biotechnol. May 7.Magneschi et al. (2012). A Mutant in the ADH1 Gene of Chlamydomonas reinhardtii Elicits Metabolic 2 Restructuring during Anaerobiosis. Plant Physiol. January 23 (ahead of print).
Special application note:
In Chlamydomonas HydA is present in low levels of 1 g/liter of culture. Therefore, an induction of cells by anaerobic adaptation or sulfur depravation (10 x higher amount than with anaerobic adaptation) is necessary for successful detection using this antibody. Methods of HydA induction are described in Hemschemeier et al. 2009.To detect HydA1/A2 in Chlamydomonas extracts amount loaded per well corresponds to 2 g of chlorophyll for sulfur deprived cells, where relatively much HydA1 is synthesized or corresponds to 2-4 g of artificially anaerobic induced cultures, where the HydA1 protein level is lower. This antibody is recognizing 1 ng of recombinant HydA protein.
Chlorophylls are one of the most abundant classes of natural pigments and have an essential role in radiant energy absorptions during photosynthesis in bacteria, algae, and higher plants. Chlorophylls occur as noncovalently bound components of pigment-protein complexes, chloroplast-localized light-harvesting antennas LHC and photosynthetic reaction centers of PSI and PSII. Upon illumination the arrest in chlorophyll biosynthesis is ended and the etiolated parts of the plant and enzymatic photoreduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide), catalysed by POR enzyme.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
POR is present in high amounts in chloroplasts not exposed to light (etioplasts),
Application Details:
1: 500 (IL), 1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
36-37 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Lee et al (2021). Chaperone-like protein DAY plays critical roles in photomorphogenesis. Nat Commun. 2021 Jul 7;12(1):4194. doi: 10.1038/s41467-021-24446-5. PMID: 34234144; PMCID: PMC8263706.Floris & K hlbrandt. (2021). Molecular landscape of etioplast inner membranes in higher plants. Nat Plants. 2021 Apr;7(4):514-523. doi: 10.1038/s41477-021-00896-z. Epub 2021 Apr 19. PMID: 33875833.Dogra et al. (2019). Oxidative post-translational modification of EXECUTER1 is required for singlet oxygen sensing in plastids. Nat Commun. 2019 Jun 27;10(1):2834. doi: 10.1038/s41467-019-10760-6. Zhang et al. (2018). Nitric oxide regulates chlorophyllide biosynthesis and singlet oxygen generation differently between Arabidopsis and barley. Nitric Oxide. 2018 Mar 3;76:6-15. doi: 10.1016/j.niox.2018.03.001.Han et al. (2015). A nuclear-encoded chloroplast-targeted S1 RNA-binding domain protein affects chloroplast rRNA processing and is crucial for the normal growth of Arabidopsis thaliana. Plant J. 2015 Jul;83(2):277-89. doi: 10.1111/tpj.12889. Epub 2015 Jun 15.
Chicken anti-Neurotrophin-4/5 (NT-4/5) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse
Immunogen:
Mixture of two human NT4/NT5 peptides (104-118 and 198-210 aa).
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA at suggested dilution of 1:500-1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Ribosomal protein L-30 is a part of 50S ribosomal large subunit, synthesized in chloroplast. Schmidt et al. (1983) Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas. J Cell Biol 96(5):1451-1463
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 %
Immunogen:
Purified native Chlamydomonas reinhardtii L-30 protein eluted from a gel piece
Cross react with L2 and L26 proteins of Chlamydomonas reinhardtii. L7/L12 is very acidic, may not bind well to nitrocellulose membrane and can have abberant mobility depending upon conditions.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Name of this antibody has been changed from L-30 | 50S ribosomal protein L30 to L7/L12 based on the following reference: Randolph-Anderson et al. (1989). Electrophoretic and immunological comparisons of chloroplast and prokaryotic ribosomal proteins reveal that certain families of large subunit proteins are evolutionarily conserved. J Mol Evol. 1989 Jul;29(1):68-88.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
OVTL12/30
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Ramaekers, F. et al. 1990 Am J Pathol 136, 641-55
References 2:
van Niekerk, CC et al. 1991 J Pathol 165, 145-52
References 3:
van de Molengraft, F.J et al. 1993 Histopathology 22, 35-8
References 4:
van Niekerk, CC et al. 1997 Cancer Detect Prev 21, 247-57
The antibody does not stain non-cornifying squamous epithelium and transitional epithelium of the bladder. In immunoblotting experiments the antibody recognizes only keratin no. 13 (54 kDa).
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
2D7
Concentration:
15 ug/ml
Storage buffer:
Culture medium with 0.01M Sodium Azide
Storage:
2-8°C
References 1:
Muijen GNP et al. (1986) Exp Cell Res 162, 97-113
References 2:
Niekerk CC van et al. (1991) J Pathol 165, 145
References 3:
Ramaekers F (1990) Am J Pathol 136, 641
References 4:
Niekerk CC van et al. (1989) Int J Cancer 43, 1065
Neuropeptide Y (NPY) is a member of a regulatory peptide family and has a marked sequence homology with pancreatic polypeptide (PP) and peptide YY (PYY), which are other members of the family. In the rat central nervous system, immunohistochemistry has found NPY-like cell bodies in the cortex, caudate-putamen, hypothalamus (arcuate nucleus), hippocampus, anterior olfactory bulb, nucleus accumbens, amygdaloid complex and periaqueductal grey. NPY-like fibers and terminals are detected in high numbers in the bed nucleus of the stria terminalis, the peri- and paraventricular regions of the hypothalamus and thalamus and in discrete hypothalamic nuclei, particularly the suprachiasmatic nucleus. It has been used to detect NPY in a wide range of species including rat, mouse, human (1-7), fish, cat, bird, guinea pig, zebrafish, squirrels, frog, and newt.
HE-10 preferably reacts with determinants of chain A type 3 and 4 and chain H type 3 and 4, but not with type 1 and 2 chain structures. It is not reactive with immunodominant A trisaccharide. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas. HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues in these individuals. HE-10 is applicable for red cell agglutination, tissue staining and immunofluorescence tests.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
HE-10
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
N?mec M. et al. Vox Sang 52:125-8 (1987)
References 2:
Vanák J. et al. Neoplasma 36(4): 479-487 (1989)
References 3:
Tichý, M. et al. Neoplasma 37(4): 451-459 (1990)
References 4:
Tichý, M. et al. Acta Univ Palacki Olomuc Fac Med. 126: 57-69 (1990)
CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Human platelets
Applications:
FC
Additional Info:
The mouse monoclonal antibody AK2 recognizes an extracellular epitope of CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d.
Clone number:
AK2
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Human platelets
Applications:
FC
Additional Info:
The mouse monoclonal antibody AK2 recognizes an extracellular epitope of CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d.
Clone number:
AK2
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Human platelets
Applications:
FC
Additional Info:
The mouse monoclonal antibody AK2 recognizes an extracellular epitope of CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d.
Clone number:
AK2
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson's disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
PBS pH 7.4, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human MANF protein produced using CHO-based cell line. Immunogen is purified from cell culture supernatant.
Applications:
WB
Antibody Isotype:
IgG1
Application Details:
Western blot (WB) at a suggested concentration of 0.5-2.0 µg/mL, Immunofluorescence 1-10 µg/mL and indirect ELISA 0.1-0.2 µg/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Store at -20°C or -70°C upon receipt. After opening maintain undiluted in smaller aliquots at -20°C or -70°C for up to 6 months. Avoid multiple freeze-thaw cycles.
MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson's disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Concentrated ammonium sulfate in PBS pH 7.4 containing no preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant human MANF protein produced using CHO-based cell line. Immunogen is purified from cell culture supernatant.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western blot (WB) at a suggested dilution of 1:2,000 - 1:6,000. Immunohistochemistry (IHC) and Immunofluorescence (IF). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
ARMET, ARP
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
MANF (Mesencephalic astrocyte-derived neurotrophic factor). No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.
Storage:
Store antibody stock solution at 2-8°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. See reconstitution instructions for more information.
CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Peripheral blood mononuclear cells of a patient suffering with CLL.
Applications:
FC,IHC
Additional Info:
The antibody HIP1 reacts with an extracellular epitope of CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d.
CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human platelets
Applications:
FC,IP,WB,ICC,ELISA
Additional Info:
The mouse monoclonal antibody AK2 recognizes an extracellular epitope of CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d.
CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human platelets
Applications:
FC,IP,WB,ICC,ELISA
Additional Info:
The mouse monoclonal antibody AK2 recognizes an extracellular epitope of CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d.
Clone number:
AK2
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: Recommended dilution: 1-4 ?g/ml.
Biosite Brand:
BioSite Flow
Conjugation:
Unconjugated
Concentration:
1 mg/ml
Species Reactivity:
human
EntrezGene ID:
2811
UniProt No:
P07359
Cookies:
X
We use cookies to help personalise and improve your web experience.
By using our website you consent to our use of cookies, some of which may have already been set on your device.
View our Cookie Policy to learn more.