MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson's disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
PBS pH 7.4, with 0.1% sodium azide.
Storage Temp:
Store at 20 °C or -70 °C upon receipt. After opening maintain undiluted in smaller aliquots at 20 °C or -70 °C for up to 6 months. Avoid multiple freeze-thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human MANF protein produced using CHO-based cell line. Immunogen is purified from cell culture supernatant.
Western blot (WB) at a suggested dilution of 1:2,000 1:4,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson's disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Concentrated ammonium sulfate in PBS pH 7.4 containing no preservatives.
Storage Temp:
Store antibody stock solution at +4 °C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. See reconstitution instructions for more information.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant human MANF protein produced using CHO-based cell line. Immunogen is purified from cell culture supernatant.
Western blot (WB) at a suggested dilution of 1:2,000 1:6,000. Immunohistochemistry (IHC) and Immunofluorescence (IF). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
ARMET, ARP
Accession Number:
P55145
Reconstitution:
Store antibody stock solution at +4°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. To use, mix well by pipetting or vortexing gently prior use, then withdraw the desired amount of antibody stock solution and dilute into your antibody dilution buffer and use normally. Alternatively, material can be centrifuged at 10K x g for 5 minutes and the liquid sulfate solution carefully removed. Then precipitated antibody can be resuspended in 0.1M P, 250mM NaCL, pH 7.4 solution. The solution should then be divided into working aliquots and stored at -80C until used. Prevent multiple freeze thaw cycles of aliquots for best product life.
Shelf Life:
12 months after purchase
Specificity:
MANF (Mesencephalic astrocyte-derived neurotrophic factor). No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB. | No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.
Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 %
Immunogen:
Purified native Chlamydomonas reinhardtii L-30 protein eluted from a gel piece
Cross react with L2 and L26 proteins of Chlamydomonas reinhardtii. L7/L12 is very acidic, may not bind well to nitrocellulose membrane and can have abberant mobility depending upon conditions.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 µl of sterile water.
Related products:
AS13 2650 | Anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies AS09 478| Anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies AS11 1738| Anti-Rpl1 | 50S ribosomal protein L1, rabbit antibodies AS15 2876 | Anti-RPL2 | ribosomal protein L2 (chloroplastic), rabbit antibodies AS14 2780 | Anti-RPL33 | 50S ribosomal protein L33, chloroplastic, rabbit antibodies AS14 2781 | Anti-RPL36 | 50S ribosomal protein L36, chloroplastic, rabbit antibodiesAS12 2115 | Anti-RPL37 | ribosomal protein L37, cytoplasmic, rabbit antibodiesAS15 2875 | Anti-RPS1 | ribosomal protein S1 (chloroplastic), rabbit antibodiesAS12 2114 | Anti-RPS12 | ribosomal protein S12, chloroplastic, rabbit antibodiesAS14 2779 | Anti-RPS15 | 30S ribosomal protein S15, chloroplastic, rabbit antibodiesAS10 719 | Anti-Rps2 | anti-ribosomal subunit 2 (cytoplasmic), rabbit antibodiesAS08 309 | Anti-S1 | anti-30S ribosomal protein S1, rabbit antibodiesAS12 2111 | Anti-S14 | 40S ribosomal protein S14-1 , rabbit antibodiesAS15 3068 | anti-S4 | mitochondrial ribosomal small subunit protein S4, rabbit antibodiesAS15 3067 | anti-S10 | mitochondrial ribosomal small subunit protein S10, rabbit antibodiesAS15 3069 | anti-L16 mitochondrial ribosomal large subunit protein L16, rabbit antibodiesCollection of antibodies to Chlamydomonas proteins
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Li et al. (2014). The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytol. 2014 Sep 25. doi: 10.1111/nph.13037.Wu et al. (2014). Large centric diatoms allocate more cellular nitrogen to photosynthesis to counter slower RUBISCO turnover rates. Front. Mar. Sci., 09 December 2014 | doi: 10.3389/fmars.2014.00068.
Special application note:
Ribosomal protein L-30 is a part of 50S ribosomal large subunit, synthesized in chloroplast. Schmidt et al. (1983) Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas. J Cell Biol 96(5):1451-1463
Jokel et al. (2020). Elimination of the flavodiiron electron sink facilitates long-term H2 photoproduction in green algae. Biotechnol Biofuels. 2019 Dec 5;12:280. doi: 10.1186/s13068-019-1618-1.Lindblad et al. (2019). CyanoFactory, a European consortium to develop technologies needed to advance cyanobacteria as chassis for production of chemicals and fuels. Algal Research, Volume 41, August 2019, 101510.Weiner at al (2018), Overcoming the expression barrier of the ferredoxin\x{2011}hydrogenase chimera in Chlamydomonas reinhardtii supports a linear increment in photosynthetic hydrogen output. Algal Research Volume 33, July 2018, Pages 310-315Wei et al. (2017). Light Intensity is Important for Hydrogen Production in NaHSO3-Treated Chlamydomonas reinhardtii. Plant Cell Physiol. 2017 Mar 1;58(3):451-457. doi: 10.1093/pcp/pcw216.Eilenberg et al. (2016). The dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux towards hydrogen production and elevated oxygen tolerance. Biotechnol Biofuels. 2016 Aug 30;9(1):182. doi: 10.1186/s13068-016-0601-3. eCollection 2016.Liran et al. (2016). Microoxic Niches within the Thylakoid Stroma of Air-Grown Chlamydomonas reinhardtii Protect [FeFe]-Hydrogenase and Support Hydrogen Production under Fully Aerobic Environment. Plant Physiol. 2016 Sep;172(1):264-71. doi: 10.1104/pp.16.01063. Epub 2016 Jul 21.Reifschneider-Wegner et al. (2014). Expression of the [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii. Int J of Hydrogen Energy, Volume 39, Issue 8, 6 March 2014, Pages 3657\x{2013}3665.Pinto et al. (2013). Rubisco mutants of Chlamydomonas reinhardtii enhance photosynthetic hydrogen production. Appl Microbiol Biotechnol. May 7.Magneschi et al. (2012). A Mutant in the ADH1 Gene of Chlamydomonas reinhardtii Elicits Metabolic 2 Restructuring during Anaerobiosis. Plant Physiol. January 23 (ahead of print).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Ostreococcus sp.
Immunogen:
Recombinant, full length Chlamydomonas reinhardtii HydA-2 Q8VZZ0
HydA1 (497aa) has a calculated MW of 53.1 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP has a calculated MW of 47.5 kDa and runs according to its size at about 48 kDa.HydA2 (505aa) has a calculated MW of 53.7 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP can only be estimated, since the cleavage site is known only from in silico analysis. It has a calculated MW of 47.3 kDa and should run in the gel also according to its size.This antibody is binding recombinant HydA1/2 protein.
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
AS09 600 | Anti-HydA | Iron-hydrogenase HydA2 rabbit antibodiesCollection of antibodies to Chlamydomonas reinhardtiiAlgal protein extraction bufferSecondary antibodies
Molecular Weight:
53.7 | 48 kDa (after transit peptide is cleaved)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
Iron-hydrogenase HydA2 is catalyzing reversible oxidation of molecular hydrogen. In Chlamydomonas the protein is present in low levels of 1 µg/liter of culture.Synonyme: HYD1/1
Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.Please do not re-use this primary antibody solution. In case of cyanobacterial samples there will be no signal in your second incubation.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, At4g32650
In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).
Application Details:
1 : 50 with 125I (WB)
Purity:
Affinity purified serum in PBS pH 7.4
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
AS09 516 | Anti-AKT1 | potassium channel AKT1, rabbit antibodiescollection of antibodies to membrane transport systemPlant protein extraction bufferSecondary antibodies
Molecular Weight:
75.5 | 75 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Lindblad et al. (2019). CyanoFactory, a European consortium to develop technologies needed to advance cyanobacteria as chassis for production of chemicals and fuels. Algal Research, Volume 41, August 2019, 101510.Weiner at al (2018), Overcoming the expression barrier of the ferredoxin\x{2011}hydrogenase chimera in Chlamydomonas reinhardtii supports a linear increment in photosynthetic hydrogen output. Algal Research Volume 33, July 2018, Pages 310-315Wei et al. (2017). Light Intensity is Important for Hydrogen Production in NaHSO3-Treated Chlamydomonas reinhardtii. Plant Cell Physiol. 2017 Mar 1;58(3):451-457. doi: 10.1093/pcp/pcw216.Eilenberg et al. (2016). The dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux towards hydrogen production and elevated oxygen tolerance. Biotechnol Biofuels. 2016 Aug 30;9(1):182. doi: 10.1186/s13068-016-0601-3. eCollection 2016.Liran et al. (2016). Microoxic Niches within the Thylakoid Stroma of Air-Grown Chlamydomonas reinhardtii Protect [FeFe]-Hydrogenase and Support Hydrogen Production under Fully Aerobic Environment. Plant Physiol. 2016 Sep;172(1):264-71. doi: 10.1104/pp.16.01063. Epub 2016 Jul 21.Reifschneider-Wegner et al. (2014). Expression of the [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii. Int J of Hydrogen Energy, Volume 39, Issue 8, 6 March 2014, Pages 3657\x{2013}3665.Pinto et al. (2013). Rubisco mutants of Chlamydomonas reinhardtii enhance photosynthetic hydrogen production. Appl Microbiol Biotechnol. May 7.Magneschi et al. (2012). A Mutant in the ADH1 Gene of Chlamydomonas reinhardtii Elicits Metabolic 2 Restructuring during Anaerobiosis. Plant Physiol. January 23 (ahead of print).
Special application note:
KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC
Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150kDa, 125kDa, 105kDa) associated with hairy cell leukaemia. Br. J. Haem. 76: 451-459
Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150kDa, 125kDa, 105kDa) associated with hairy cell leukaemia. Br. J. Haem. 76: 451-459
Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150kDa, 125kDa, 105kDa) associated with hairy cell leukaemia. Br. J. Haem. 76: 451-459
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