Iron-hydrogenase HydA2 is catalyzing reversible oxidation of molecular hydrogen. In Chlamydomonas the protein is present in low levels of 1 µg/liter of culture.Synonyme: HYD1/1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Ostreococcus sp.
Immunogen:
Recombinant, full length Chlamydomonas reinhardtii HydA-2 Q8VZZ0
HydA1 (497aa) has a calculated MW of 53.1 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP has a calculated MW of 47.5 kDa and runs according to its size at about 48 kDa.HydA2 (505aa) has a calculated MW of 53.7 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP can only be estimated, since the cleavage site is known only from in silico analysis. It has a calculated MW of 47.3 kDa and should run in the gel also according to its size.This antibody is binding recombinant HydA1/2 protein.
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
AS09 600 | Anti-HydA | Iron-hydrogenase HydA2 rabbit antibodiesCollection of antibodies to Chlamydomonas reinhardtiiAlgal protein extraction bufferSecondary antibodies
Molecular Weight:
53.7 | 48 kDa (after transit peptide is cleaved)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Lindblad et al. (2019). CyanoFactory, a European consortium to develop technologies needed to advance cyanobacteria as chassis for production of chemicals and fuels. Algal Research, Volume 41, August 2019, 101510.Weiner at al (2018), Overcoming the expression barrier of the ferredoxin\x{2011}hydrogenase chimera in Chlamydomonas reinhardtii supports a linear increment in photosynthetic hydrogen output. Algal Research Volume 33, July 2018, Pages 310-315Wei et al. (2017). Light Intensity is Important for Hydrogen Production in NaHSO3-Treated Chlamydomonas reinhardtii. Plant Cell Physiol. 2017 Mar 1;58(3):451-457. doi: 10.1093/pcp/pcw216.Eilenberg et al. (2016). The dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux towards hydrogen production and elevated oxygen tolerance. Biotechnol Biofuels. 2016 Aug 30;9(1):182. doi: 10.1186/s13068-016-0601-3. eCollection 2016.Liran et al. (2016). Microoxic Niches within the Thylakoid Stroma of Air-Grown Chlamydomonas reinhardtii Protect [FeFe]-Hydrogenase and Support Hydrogen Production under Fully Aerobic Environment. Plant Physiol. 2016 Sep;172(1):264-71. doi: 10.1104/pp.16.01063. Epub 2016 Jul 21.Reifschneider-Wegner et al. (2014). Expression of the [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii. Int J of Hydrogen Energy, Volume 39, Issue 8, 6 March 2014, Pages 3657\x{2013}3665.Pinto et al. (2013). Rubisco mutants of Chlamydomonas reinhardtii enhance photosynthetic hydrogen production. Appl Microbiol Biotechnol. May 7.Magneschi et al. (2012). A Mutant in the ADH1 Gene of Chlamydomonas reinhardtii Elicits Metabolic 2 Restructuring during Anaerobiosis. Plant Physiol. January 23 (ahead of print).
Special application note:
In Chlamydomonas HydA is present in low levels of 1 µg/liter of culture. Therefore, an induction of cells by anaerobic adaptation or sulfur depravation (10 x higher amount than with anaerobic adaptation) is necessary for successful detection using this antibody. Methods of HydA induction are described in Hemschemeier et al. 2009.To detect HydA1/A2 in Chlamydomonas extracts amount loaded per well corresponds to 2 µg of chlorophyll for sulfur deprived cells, where relatively much HydA1 is synthesized or corresponds to 2-4 µg of artificially anaerobic induced cultures, where the HydA1 protein level is lower. This antibody is recognizing 1 ng of recombinant HydA protein.
Ribosomal protein L-30 is a part of 50S ribosomal large subunit, synthesized in chloroplast. Schmidt et al. (1983) Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas. J Cell Biol 96(5):1451-1463
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 %
Immunogen:
Purified native Chlamydomonas reinhardtii L-30 protein eluted from a gel piece
Cross react with L2 and L26 proteins of Chlamydomonas reinhardtii. L7/L12 is very acidic, may not bind well to nitrocellulose membrane and can have abberant mobility depending upon conditions.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 µl of sterile water.
Related products:
AS13 2650 | Anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies AS09 478| Anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies AS11 1738| Anti-Rpl1 | 50S ribosomal protein L1, rabbit antibodies AS15 2876 | Anti-RPL2 | ribosomal protein L2 (chloroplastic), rabbit antibodies AS14 2780 | Anti-RPL33 | 50S ribosomal protein L33, chloroplastic, rabbit antibodies AS14 2781 | Anti-RPL36 | 50S ribosomal protein L36, chloroplastic, rabbit antibodiesAS12 2115 | Anti-RPL37 | ribosomal protein L37, cytoplasmic, rabbit antibodiesAS15 2875 | Anti-RPS1 | ribosomal protein S1 (chloroplastic), rabbit antibodiesAS12 2114 | Anti-RPS12 | ribosomal protein S12, chloroplastic, rabbit antibodiesAS14 2779 | Anti-RPS15 | 30S ribosomal protein S15, chloroplastic, rabbit antibodiesAS10 719 | Anti-Rps2 | anti-ribosomal subunit 2 (cytoplasmic), rabbit antibodiesAS08 309 | Anti-S1 | anti-30S ribosomal protein S1, rabbit antibodiesAS12 2111 | Anti-S14 | 40S ribosomal protein S14-1 , rabbit antibodiesAS15 3068 | anti-S4 | mitochondrial ribosomal small subunit protein S4, rabbit antibodiesAS15 3067 | anti-S10 | mitochondrial ribosomal small subunit protein S10, rabbit antibodiesAS15 3069 | anti-L16 mitochondrial ribosomal large subunit protein L16, rabbit antibodiesCollection of antibodies to Chlamydomonas proteins
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
Name of this antibody has been changed from L-30 | 50S ribosomal protein L30 to L7/L12 based on the following reference: Randolph-Anderson et al. (1989). Electrophoretic and immunological comparisons of chloroplast and prokaryotic ribosomal proteins reveal that certain families of large subunit proteins are evolutionarily conserved. J Mol Evol. 1989 Jul;29(1):68-88.
Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150kDa, 125kDa, 105kDa) associated with hairy cell leukaemia. Br. J. Haem. 76: 451-459
Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150kDa, 125kDa, 105kDa) associated with hairy cell leukaemia. Br. J. Haem. 76: 451-459
Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150kDa, 125kDa, 105kDa) associated with hairy cell leukaemia. Br. J. Haem. 76: 451-459
Cookies:
X
We use cookies to help personalise and improve your web experience.
By using our website you consent to our use of cookies, some of which may have already been set on your device.
View our Cookie Policy to learn more.
