Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. CDH23 is required for establishing and/or maintaining the proper organization of the stereocilia bundle of hair cells in the cochlea and the vestibule during late embryonic/early postnatal development. It is part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing.
CDH23 Antibody detects endogenous levels of total CDH23
Immunogen:
Fusion protein of human CDH23
Cross Reactivity:
Human,Mouse,Rat
Conjugate:
Unconjugated
purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink(TM) Coupling Resin (Thermo Fisher Scientific).
Concentration:
1mg/ml
Fragment:
Fab fragment
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:12559562, PubMed:15203199). Via association with PRDM14 is involved in regulation of embryonic stem cell (ESC) pluripotency (PubMed:27281218). Involved in primordial germ cell (PCG) formation. Stabilizes PRDM14 and OCT4 on chromatin in a homooligomerization-dependent manner (By similarity). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed:23251453). May function as a complex with the chimeric protein RUNX1/AML1-CBFA2T1/MTG8 (AML1-MTG8/ETO fusion protein) which is produced in acute myeloid leukemia with the chromosomal translocation t(8;21). May thus be involved in the repression of AML1-dependent transcription and the induction of G-CSF/CSF3-dependent cell growth. May be a tumor suppressor gene candidate involved in myeloid tumors with the deletion of the 20q11 region. Through heteromerization with CBFA2T3/MTG16 may be involved in regulation of the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (By similarity). Required for the maintenance of the secretory cell lineage in the small intestine. Can inhibit Notch signaling probably by association with RBPJ and may be involved in GFI1-mediated Paneth cell differentiation (By similarity).
CBFA2T2 Antibody detects endogenous levels of CBFA2T2.
Immunogen:
A synthesized peptide derived from human CBFA2T2.
Cross Reactivity:
Human,Mouse,Rat
Conjugate:
Unconjugated
purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Concentration:
1mg/ml
Fragment:
Fab fragment
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation.
FLII Antibody detects endogenous levels of total FLII
Immunogen:
A synthesized peptide derived from human FLII
Cross Reactivity:
Human
Conjugate:
Unconjugated
purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink(TM) Coupling Resin (Thermo Fisher Scientific).
Concentration:
1mg/ml
Fragment:
Fab fragment
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1 (PubMed:23251453).
ZBTB33 Antibody detects endogenous levels of total ZBTB33
Immunogen:
A synthesized peptide derived from human ZBTB33
Cross Reactivity:
Human,Mouse,Rat
Conjugate:
Unconjugated
purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink(TM) Coupling Resin (Thermo Fisher Scientific).
Concentration:
1mg/ml
Fragment:
Fab fragment
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.
DUSP3 Antibody detects endogenous levels of total DUSP3
Immunogen:
A synthesized peptide derived from human DUSP3
Cross Reactivity:
Human,Mouse
Conjugate:
Unconjugated
purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink(TM) Coupling Resin (Thermo Fisher Scientific).
Concentration:
1mg/ml
Fragment:
Fab fragment
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
PRRX2 Antibody detects endogenous levels of total PRRX2
Immunogen:
A synthesized peptide derived from human PRRX2
Cross Reactivity:
Human,Mouse,Rat
Conjugate:
Unconjugated
purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink(TM) Coupling Resin (Thermo Fisher Scientific).
Concentration:
1mg/ml
Fragment:
Fab fragment
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info:
Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
Product Type:
Antibodies
Format:
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage Temp:
-20ºC
Host Animal:
Rabbit
Species Reactivity:
Hu | Ms | Rt | Bv | Mk | Ck | Dg | GP | Hm | Pg | Qu | Rb | Sh | Dr | Xe
Immunogen:
Dog calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Applications:
WB | ICC/IF | IHC
Application Details:
WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Research Area(s):
Cell Signaling | Tags and Cell Markers | Organelle Markers
Target:
Calnexin
Conjugate:
Unconjugated
Alternative Name(s):
Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, P90 antibody
1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry 33: 3229. 3. Schrag J. et al. (2001) Molecular Cell 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.
This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info:
Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
Product Type:
Antibodies
Format:
Rabbit Antiserum
Storage Temp:
-20ºC
Host Animal:
Rabbit
Species Reactivity:
Hu | Ms | Rt | Bv | Ck | Dg | GP | Hm | Pg | Mk | Rb | Sh | Xe
Immunogen:
A 19 residue synthetic peptide based on dog calnexin and the peptide coupled to KLH
Applications:
WB | IHC | ICC/IF | IP
Application Details:
WB (1:5000), IHC (1:100), ICC/IF (1:100), IP (1:100); optimal dilutions for assays should be determined by the user.
Research Area(s):
Cell Signaling | Tags and Cell Markers | Organelle Markers
Target:
Calnexin
Conjugate:
Unconjugated
Alternative Name(s):
Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, P90 antibody
1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry 33:3229. 3. Schrag J. et al. (2001) Molecular Cell 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49):40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6. 8. Raggo C., et al. (2002) Mol Cell Biol. 22: 5639-5649. 9. Rubio M.E., and Wenthold R.J. (1999) J Neurochem. 73: 942-948.
This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info:
Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
Product Type:
Antibodies
Format:
PBS pH7.2, 50% glycerol, 0.09% sodium azide
Storage Temp:
-20ºC
Host Animal:
Rabbit
Species Reactivity:
Hu | Ms | Rt | Bv | Mk | Ck | Dg | GP | Hm | Pg | Qu | Rb | Sh | Dr | Xe
Immunogen:
Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Applications:
WB | IHC | ICC/IF | IP | FCM
Application Details:
WB (1:2000), ICC/IF (1:100), IHC (1:100); optimal dilutions for assays should be determined by the user.
Research Area(s):
Cell Signaling | Tags and Cell Markers | Organelle Markers
Target:
Calnexin
Conjugate:
Unconjugated
Alternative Name(s):
Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, P90 antibody
1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science. 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry. 33: 3229. 3. Schrag J. et al. (2001) Molecular Cell. 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.
The Glutathione S-Transferase (GST) family of isozymes function to detoxify and neutralize a wide variety of electrophilic molecules by mediating their conjugation with reduced glutathione (1). Human GSTs are encoded by 5 gene families, expressing in almost all tissues as four cytosolic and one microsomal forms. Dividing the family by isoelectric points, the basic alpha (pI 811), the neutral mu (pI 57) and acidic pi (pH<5) classes are populated by additional subclasses, each isozyme displaying differential specificity for given electrophilic molecules (2). Given its pivotal role in ameliorating oxidative stress/damage, GST activity has been repeatedly investigated as a biomarker for arthritis, asthma, COPD, and multiple forms of cancer, as well as an environmental marker (3-7). Examination of GST isoforms and activity in human cancers, tumors and tumor cell lines has revealed the predominance of the acidic pi class. Furthermore, this activity is thought to substantially contribute to the innate or acquired resistance of specific neoplasms to anticancer therapy (8,9).
Product Type:
Assay Kits
Storage Temp:
4ºC
Species Reactivity:
Hu | Mlsk | Fs
Applications:
Fluorometric assay used to quantitatively measure the activity of glutathione S-transferase in samples.
Human | Mollusk | Fish | Toadfish (Batrachoididae) | Oyster
References:
1. Habig, W, et al. J. Biol.Chem. 1974 249(22):7130-7139. 2. Cook, JA, et al. Cancer Res. 1991 51:1606-1612. 3. Dalle-Donne, I, et al. 2006 52(4):601-623. 4. Surapneni, KM & VSC Gopan. Ind.J.Clin.Biochem. 2008 23(1):41.44. 5. Mohan, SK & O Venkataramana. Ind.J.Med. Sci. 2007 61:9-14. 6. Ferrandina, G., et al. Ann. Oncol. 1997 8:343-350. 7. Otitoju, O & Onwarah, INE. African J. Biotech. 2007 6(12)1455-1459. 8. Shea, TC, et al. Cancer Res. 1988 48:527-533. 9. Shea, TC, et al. Cancer Res. 1990 50:6848-6853.
The Glutathione S-Transferase Activity kit is designed to quantitatively measure the activity of GST present in a variety of samples. A GST standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. The kit utilizes a non-fluorescent molecule that is a substrate for the GST enzyme which covalently attaches to glutathione (GSH) to yield a highly fluorescent product. Mixing the sample or standard with the supplied Detection Reagent and GSH and incubating at room temperature for 30 minutes yields a fluorescent product which is read at 460 nm in a fluorescent plate reader with excitation at 390 nm. The activity of the GST in the sample is calculated, after making a suitable correction for any dilution of the sample, using software available with most fluorescence plate readers. We have provided a 96 well plate for measurement but this assay is adaptable for higher density plate formats. The end user should ensure that their HTS black plate is suitable for use with these reagents prior to running samples.
Kit Components:
Black Half Area 96 Well Plate | Glutathione S-Transferase Standard | GST Detection Reagent | Dry DMSO | Assay Buffer | Glutathione (GSH)
Precision:
Intra Assay Precision:Four serum samples were diluted 1:2 with Assay Buffer and run in replicates of 16 in an assay. The mean and precision of the calculated GST activities were: Sample 1- 315.9 mU/mL, 4.6% CV Sample 2- 221.2 mU/mL, 5.6% CV Sample 3- 88.2 mU/mL, 4.2% CV Sample 4- 22.7 mU/mL, 6.6% CV Inter Assay Precision: Four serum samples were diluted 1:2 with Assay Buffer and run in duplicates in twenty assays over multiple days by four operators. The mean and precision of the calculated GST activities were: Sample 1- 291.7 mU/mL, 12.6% CV Sample 2- 218.5 mU/mL, 11.0% CV Sample 3- 89.6 mU/mL, 10.4% CV Sample 4- 23.0 mU/mL, 15.9% CV
Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207'. Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation. Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose.
BLK (Phospho-Tyr389) Antibody detects endogenous levels of BLK only when phosphorylated at Tyr389
Epitope:
Phospho Tyr389
Immunogen:
A synthesized peptide derived from human BLK (Phospho-Tyr389)
Cross Reactivity:
Human,Mouse,Rat
Conjugate:
Unconjugated
purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Concentration:
1mg/ml
Fragment:
Fab fragment
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207'. Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation. Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose.
BLK antibody detects endogenous levels of total BLK.
Immunogen:
Purified recombinant fragment of human BLK expressed in E. Coli.
Cross Reactivity:
Human
Conjugate:
Unconjugated
purification:
Affinity-chromatography.
Concentration:
1mg/ml
Fragment:
Fab fragment
Buffer:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Ribosomal protein L-30 is a part of 50S ribosomal large subunit, synthesized in chloroplast. Schmidt et al. (1983) Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas. J Cell Biol 96(5):1451-1463
Product Type:
Protein standard
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 %
Immunogen:
Purified native Chlamydomonas reinhardtii L-30 protein eluted from a gel piece
Cross react with L2 and L26 proteins of Chlamydomonas reinhardtii. L7/L12 is very acidic, may not bind well to nitrocellulose membrane and can have abberant mobility depending upon conditions.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 µl of sterile water.
Related products:
AS13 2650 | Anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies AS09 478| Anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies AS11 1738| Anti-Rpl1 | 50S ribosomal protein L1, rabbit antibodies AS15 2876 | Anti-RPL2 | ribosomal protein L2 (chloroplastic), rabbit antibodies AS14 2780 | Anti-RPL33 | 50S ribosomal protein L33, chloroplastic, rabbit antibodies AS14 2781 | Anti-RPL36 | 50S ribosomal protein L36, chloroplastic, rabbit antibodiesAS12 2115 | Anti-RPL37 | ribosomal protein L37, cytoplasmic, rabbit antibodiesAS15 2875 | Anti-RPS1 | ribosomal protein S1 (chloroplastic), rabbit antibodiesAS12 2114 | Anti-RPS12 | ribosomal protein S12, chloroplastic, rabbit antibodiesAS14 2779 | Anti-RPS15 | 30S ribosomal protein S15, chloroplastic, rabbit antibodiesAS10 719 | Anti-Rps2 | anti-ribosomal subunit 2 (cytoplasmic), rabbit antibodiesAS08 309 | Anti-S1 | anti-30S ribosomal protein S1, rabbit antibodiesAS12 2111 | Anti-S14 | 40S ribosomal protein S14-1 , rabbit antibodiesAS15 3068 | anti-S4 | mitochondrial ribosomal small subunit protein S4, rabbit antibodiesAS15 3067 | anti-S10 | mitochondrial ribosomal small subunit protein S10, rabbit antibodiesAS15 3069 | anti-L16 mitochondrial ribosomal large subunit protein L16, rabbit antibodiesCollection of antibodies to Chlamydomonas proteins
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
Name of this antibody has been changed from L-30 | 50S ribosomal protein L30 to L7/L12 based on the following reference: Randolph-Anderson et al. (1989). Electrophoretic and immunological comparisons of chloroplast and prokaryotic ribosomal proteins reveal that certain families of large subunit proteins are evolutionarily conserved. J Mol Evol. 1989 Jul;29(1):68-88.
Iron-hydrogenase HydA2 is catalyzing reversible oxidation of molecular hydrogen. In Chlamydomonas the protein is present in low levels of 1 µg/liter of culture.Synonyme: HYD1/1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Ostreococcus sp.
Immunogen:
Recombinant, full length Chlamydomonas reinhardtii HydA-2 Q8VZZ0
HydA1 (497aa) has a calculated MW of 53.1 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP has a calculated MW of 47.5 kDa and runs according to its size at about 48 kDa.HydA2 (505aa) has a calculated MW of 53.7 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP can only be estimated, since the cleavage site is known only from in silico analysis. It has a calculated MW of 47.3 kDa and should run in the gel also according to its size.This antibody is binding recombinant HydA1/2 protein.
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
AS09 600 | Anti-HydA | Iron-hydrogenase HydA2 rabbit antibodiesCollection of antibodies to Chlamydomonas reinhardtiiAlgal protein extraction buffer Secondary antibodies
Molecular Weight:
53.7 | 48 kDa (after transit peptide is cleaved)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Lindblad et al. (2019). CyanoFactory, a European consortium to develop technologies needed to advance cyanobacteria as chassis for production of chemicals and fuels. Algal Research, Volume 41, August 2019, 101510. Weiner at al (2018), Overcoming the expression barrier of the ferredoxinâhydrogenase chimera in Chlamydomonas reinhardtii supports a linear increment in photosynthetic hydrogen output. Algal Research Volume 33, July 2018, Pages 310-315 Wei et al. (2017). Light Intensity is Important for Hydrogen Production in NaHSO3-Treated Chlamydomonas reinhardtii. Plant Cell Physiol. 2017 Mar 1;58(3):451-457. doi: 10.1093/pcp/pcw216. Eilenberg et al. (2016). The dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux towards hydrogen production and elevated oxygen tolerance. Biotechnol Biofuels. 2016 Aug 30;9(1):182. doi: 10.1186/s13068-016-0601-3. eCollection 2016.Liran et al. (2016). Microoxic Niches within the Thylakoid Stroma of Air-Grown Chlamydomonas reinhardtii Protect [FeFe]-Hydrogenase and Support Hydrogen Production under Fully Aerobic Environment. Plant Physiol. 2016 Sep;172(1):264-71. doi: 10.1104/pp.16.01063. Epub 2016 Jul 21.Reifschneider-Wegner et al. (2014). Expression of the [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii. Int J of Hydrogen Energy, Volume 39, Issue 8, 6 March 2014, Pages 3657â3665.Pinto et al. (2013). Rubisco mutants of Chlamydomonas reinhardtii enhance photosynthetic hydrogen production. Appl Microbiol Biotechnol. May 7. Magneschi et al. (2012). A Mutant in the ADH1 Gene of Chlamydomonas reinhardtii Elicits Metabolic 2 Restructuring during Anaerobiosis. Plant Physiol. January 23 (ahead of print).
Special application note:
In Chlamydomonas HydA is present in low levels of 1 µg/liter of culture. Therefore, an induction of cells by anaerobic adaptation or sulfur depravation (10 x higher amount than with anaerobic adaptation) is necessary for successful detection using this antibody. Methods of HydA induction are described in Hemschemeier et al. 2009.To detect HydA1/A2 in Chlamydomonas extracts amount loaded per well corresponds to 2 µg of chlorophyll for sulfur deprived cells, where relatively much HydA1 is synthesized or corresponds to 2-4 µg of artificially anaerobic induced cultures, where the HydA1 protein level is lower. This antibody is recognizing 1 ng of recombinant HydA protein.
MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson's disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
PBS pH 7.4, with 0.1% sodium azide.
Storage Temp:
Store at 20 °C or -70 °C upon receipt. After opening maintain undiluted in smaller aliquots at 20 °C or -70 °C for up to 6 months. Avoid multiple freeze-thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human MANF protein produced using CHO-based cell line. Immunogen is purified from cell culture supernatant.
Western blot (WB) at a suggested dilution of 1:2,000 1:4,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson's disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Concentrated ammonium sulfate in PBS pH 7.4 containing no preservatives.
Storage Temp:
Store antibody stock solution at +4 °C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. See reconstitution instructions for more information.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant human MANF protein produced using CHO-based cell line. Immunogen is purified from cell culture supernatant.
Western blot (WB) at a suggested dilution of 1:2,000 1:6,000. Immunohistochemistry (IHC) and Immunofluorescence (IF). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
ARMET, ARP
Accession Number:
P55145
Reconstitution:
Store antibody stock solution at +4°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. To use, mix well by pipetting or vortexing gently prior use, then withdraw the desired amount of antibody stock solution and dilute into your antibody dilution buffer and use normally. Alternatively, material can be centrifuged at 10K x g for 5 minutes and the liquid sulfate solution carefully removed. Then precipitated antibody can be resuspended in 0.1M P, 250mM NaCL, pH 7.4 solution. The solution should then be divided into working aliquots and stored at -80C until used. Prevent multiple freeze thaw cycles of aliquots for best product life.
Shelf Life:
12 months after purchase
Specificity:
MANF (Mesencephalic astrocyte-derived neurotrophic factor). No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB. | No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.
Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150kDa, 125kDa, 105kDa) associated with hairy cell leukaemia. Br. J. Haem. 76: 451-459
Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150kDa, 125kDa, 105kDa) associated with hairy cell leukaemia. Br. J. Haem. 76: 451-459
Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150kDa, 125kDa, 105kDa) associated with hairy cell leukaemia. Br. J. Haem. 76: 451-459
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