Clones AE1 and AE3 are specific for the 56.5, 50, 50', 48 and 40 kD acidic cytokeratins as well as the 65 to 67, 64, 59, 58, 56 and 52 kD basic cytokeratins. The cocktail of clones AE1 and AE3 exhibit broad reactivity with two families of cytokeratin, acidic and basic.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
AE1/AE3
Concentration:
Greater than or equal to 225 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Nadji M, Morales AR. Laboratory Medicine. 1983; 14:767
References 2:
Omata M et al. Am.J.of Clin. Pathol. 1980; 73:626
References 3:
Su T et al. Diagnostic Pathology. 2014; 9: 179
References 4:
Zhao W et al. Int.J. of Clin. and Exp.Pathol. 2014; 7(11): 7951-7956
References 5:
Hammers HJ et al. Molecular Cancer Therapeutics. 2010; 9(6): 1525-1535
Mouse epsilon heavy chain of immunoglobulin (determined by immunodot). Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic) and immunohistochemistry on frozen sections. Available in unconjugated MON5051, FITC conjugated MON5051F, HRP conjugated MON5050P and Biotin conjugated MON5051B.
Mouse epsilon heavy chain of immunoglobulin (determined by immunodot). Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic) and immunohistochemistry on frozen sections. Available in unconjugated MON5051, FITC conjugated MON5051F, HRP conjugated MON5050P and Biotin conjugated MON5051B.
Mouse epsilon heavy chain of immunoglobulin (determined by immunodot). Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic) and immunohistochemistry on frozen sections. Available in unconjugated MON5051, FITC conjugated MON5051F, HRP conjugated MON5050P and Biotin conjugated MON5051B.
Mouse epsilon heavy chain of immunoglobulin (determined by immunodot). Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic) and immunohistochemistry on frozen sections. Available in unconjugated MON5051, FITC conjugated MON5051F, HRP conjugated MON5050P and Biotin conjugated MON5051B.
Mouse alpha heavy chain of immunoglobulin (determined by immunodot). Avidity on IgA: 2.9 * 109 M-1. Available as unconjugated MON5050, Biotin conjugated MON5050B, Peroxidase conjugated MON5050P.
Antibody Isotype:
IgMk
Monosan Range:
MONOSAN
Clone:
LO-MA-7
Concentration:
1 mg/ml
Storage buffer:
PBS with 0.1% sodium azide 50% glycerol
Storage:
-20°C
References 1:
Bazin et al. Pergamon Press Oxford and NY 1982; 29:615-618
Mouse alpha heavy chain of immunoglobulin (determined by immunodot). Avidity on IgA: 4.8 * 109 M-1. Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic) and immunohistochemistry on frozen sections. Available in unconjugated MON5050, HRP conjugated MON5050P, Biotin conjugated MON5050B
Mouse alpha heavy chain of immunoglobulin (determined by immunodot). Avidity on IgA: 4.8 * 109 M-1. Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic) and immunohistochemistry on frozen sections. Available in unconjugated MON5050, HRP conjugated MON5050P, Biotin conjugated MON5050B
Goat anti-chicken IgY (H&L) is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to chicken IgY in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Chicken IgY heavy and light chains (H&L).
Immunogen:
Purified chicken IgY, whole molecule
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Antibody binds to:heavy chains on chicken IgYlight chains on all chicken immunoglobulinsNo reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.Chicken immunoglobulin is often called hen or chicken IgG, however it is derived from egg yolk, therefore IgY.
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Bindari et al. (2020). Methods to prevent PCR amplification of DNA from non-viable virus were not successful for infectious laryngotracheitis virus. PLoS One. 2020 May 22;15(5):e0232571. doi: 10.1371/journal.pone.0232571. PMID: 32442180; PMCID: PMC7244108.Levitan et al. (2019). Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17316-17322. doi: 10.1073/pnas.1906726116.Heard et al. (2015). Identification of Regulatory and Cargo Proteins of Endosomal and Secretory Pathways in Arabidopsis thaliana by Proteomic Dissection. Mol Cell Proteomics. 2015 Jul;14(7):1796-813. doi: 10.1074/mcp.M115.050286. Epub 2015 Apr 21.Huang et al. (2015). Effects of exogenous salicylic acid on the physiological characteristics of Dendrobium officinale under chilling stress. Plant Growth Regulation pp 1-10.
Special application note:
Concentration: 1.0 mg/mlThis HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative.
Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Antibody can be used to check qubiquitination status in the whole plant extracts.Technical note: It is very difficult to detect ubiquitin monomers in total cell extracts due to a great abundance of poly and multi-ubiquitinated proteins. Recommended is size separation of protein extracts before gel electrophoresis focused on good resolution of region between 6-10 kDa.Suggested extraction buffer: 100 mM Tris-HCl, pH 8.0, 0.1% [w/v] SDS, 0.5% [w/v] sodium deoxycholate, 1% [v/v] glycerol, 50 mM sodium metabisulfite, 20���mM N-ethylmaleimide (NEM) and protease inhibitor cocktail (Roche) (Orosa et al. 2018). This buffer will help to stabilize the conjugates and will help to detect any increase or decrease in conjugate accumulation using the antibodies.
Application Details:
1 : 10 000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
8,5 kDa
Not reactive in:
Algae
Selected references:
Jeran et al. (2021) The PUB4 E3 Ubiquitin Ligase Is Responsible for the Variegated Phenotype Observed upon Alteration of Chloroplast Protein Homeostasis in Arabidopsis Cotyledons. Genes (Basel). 2021 Sep 6;12(9):1387. doi: 10.3390/genes12091387. PMID: 34573369; PMCID: PMC8464772.Filippi et al. (2019). Caspase-3-like activity and proteasome degradation in grapevine suspension cell cultures undergoing silver-induced programmed cell death. J Plant Physiol. 2019 Feb;233:42-51. doi: 10.1016/j.jplph.2018.12.003.Krasuska et al. (2016). Nitric oxide-polyamines cross-talk during dormancy release and germination of apple embryos. Nitric Oxide. 2016 Nov 23. pii: S1089-8603(16)30178-1. doi: 10.1016/j.niox.2016.11.003. [Epub ahead of print]
Special application note:
Ubiquitin mutant is lethal, therefore could not be used in validation of this antibody.
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Nannochloropsis gaditana, CyanobacteriaSpecies of your interest not listed? Contact us
Immunogen:
Recombinant maize chloroplast AtpA P05022
Applications:
Immunogold (IG), Immunoprecipitation (IP), Western blot (WB)
Pao et al. (2018). Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning. J Plant Res. 2018 Mar 2. doi: 10.1007/s10265-018-1020-2. (ImmunoGold)Zhang et al. (2017). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.Jeon et al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y.Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016.Camejo et al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370.Yang et al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006.
Special application note:
Sequence of the protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757
This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.;
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Host Animal:
mouse
Immunogen:
Purified recombinant fragment of human NOX4 (AA: 210-310) expressed in E. Coli.
This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.;
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Host Animal:
mouse
Immunogen:
Purified recombinant fragment of human NOX4 (AA: 210-310) expressed in E. Coli.
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