Product Detail: |
Quantitative colorimetric measurement of urea nitrogen |
Background Info: |
Urea is a by-product of protein metabolism by the liver, and is therefore removed from the blood by the kidneys. Urea freely filters through the glomerulous, but is reabsorbed by the renal tubules in a flow-dependent fashion. The higher the flow rate, the greater amount of urea nitrogen is cleared from circulation and eliminated through the kidneys. As a result, the level of circulating urea nitrogen, along with serum creatinine, serves as a primary measure of kidney function. Normal adult Blood Urea Nitrogen (BUN) levels should be between 7 and 21 mg urea nitrogen per 100 mL blood (mg/dL) (1). Azotemia, poor kidney function, will cause elevated BUN levels (? 50 mg/dL) and is associated with acute kidney failure or injury, severe acute pancreatitis, congestive heart failure or gastrointestinal bleeding (2-5). Azotemia also can occur with dehydration, as a result of alcohol abuse, or high protein diets. Lower than expected BUN levels are usually not clinically predictive, but are primarily associated with liver disease or malnutrition, including malabsorption and low protein diets (6). Urine and saliva are considered to be acceptable non-invasive samples for measurement of urea nitrogen (7). Serum creatinine is another metabolic waste product freely filtered by the glumerulous, but does not undergo tubular reabsorption. Its steady rate of elimination is frequently used to generate an index or ratio with BUN values for normalized evaluations. Creatinine Urinary Detection Kit (SKT-200) and Creatinine Serum Detection Kit (SKT-217) are also available from StressMarq Biosciences. |
Product Type: |
Detection Kits |
Storage Temp: |
4ºC |
Species Reactivity: |
ALL |
Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
Target: |
Blood Urea Nitrogen |
Research Area(s): |
Cell Signaling | Cardiovascular System |
Alternative Name(s): |
Blood urea nitrogen Detection Kit |
Category: |
Assay Kits |
Species Reactivity Full Name: |
Species Independent |
Shipping Temperature: |
Blue Ice |
References: |
1. Laboratory reference values. Urea nitrogen (BUN). Rochester, Minn.: Mayo Foundation for Medical Education and Research; Nov. 2010. 2. Waiker, SS and JV Bonventre. (2008) Nephron Clin. Pract. 109:c192-c197. 3. Al Mofleh, IA. World J. Gastroent. (2008) Congestive heart failure. 14(5):675-684. 4. Iglesiase, J. et al. (2006) Nephrol. Dial. Transplant. 21:3458-3465. 5. Mayo Clinic. Blood urea nitrogen (BUN) tests. http://www.mayoclinic.com/health/blood-urea-nitrogen/MY00373/DSECTION=results 6. Lum, G and S Leal-Khouri. (1989) Clin. Chem. 35(4):639-640. 7. Akai, T, et al. (1983) Clin. Chem. 1983. 29(10):1825-1827. |
Launch Date: |
17/Sep/2014 |
Tarriff Code: |
3822.00.1090 |
ADR Code: |
Non-hazardous |
UN Code for transport: |
Non-hazardous |
Country of Origin: |
Canada |
Assay Type: |
Direct Quantitative Assay |
Assay Type Abbreviation: |
Direct |
Platform: |
Microplate |
Utility: |
Colorimetric assay used to quantitatively measure urea nitrogen in a variety of samples. |
Assay Overview: |
The Blood Urea Nitrogen (BUN) Detection Kit is designed to quantitatively measure urea nitrogen in a variety of samples. A urea nitrogen standard calibrated to NIST reference materials is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with Color Reagents A and B and incubated at room temperature for 30 minutes. The colored product is read at 450 nm. The concentration of urea nitrogen in the sample is calculated, after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of mg/dL urea nitrogen. If samples are to be expressed in terms of mg/dL urea, the data can be converted using the multiplier 2.14. |
Kit Components: |
Clear 96 Well Plates | Urea Nitrogen Standard | Color Reagent A | Color Reagent B |
Precision: |
Inter Assay Precision: Three human samples were further diluted in water and run in duplicates in twenty-eight assays run over multiple days by five operators. The mean and precision of the calculated concentrations were: Sample 1- 1.29 mg/dL, 3.1% CV Sample 2- 2.35 mg/dL, 4.3% CV Sample 3- 5.18 mg/dL, 3.3% CV |
Sample Type(s): |
Serum | Plasma | Urine | Saliva | Tissue Culture Media |
Number of Samples: |
88 samples in duplicate |
Incubation Time: |
30 Minutes |
Sensitivity: |
0.030 mg/dl |
Assay Range: |
0.156 - 10 mg/dl |
Cite this Product: |
Blood Urea Nitrogen Detection Kit (StressMarq Biosciences, Canada, Cat # SKT-213-192) |
PubChem CID: |
28396669 |
Trademark: |
StressXpress® |