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ImmunoStar 20064 100uL £275.00
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Product Detail: Substance P Antibody
Background Info: Raised to Substance P coupled to KLH with carbodiimide .
Product Type: Antibody - Antibodies
Antibody Type: polyclonal
Format: Lyophilised
Host Animal: Rabbit
Species Reactivity: Rat
Immunogen: Substance P
Applications: Immunohistochemistry, Immunocytochemistry, immunofluorescence
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
Additional Info: The Substance P antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat substantia nigra and spinal cord using biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500-1/1000 in PBS/0.3% Triton X-100 - Cy3 Fluorchrome and 1/6000-1/8000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique.

The specificity of the antiserum for Substance P was demonstrated using soluble pre-adsorption with the peptides in question at a final concentration of 10 µg of peptide per mL of diluted antiserum. Substance P immunolabeling was completely abolished by pre-adsorption with Substance P. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: neurokinin A, neurokinin B, somatostatin and neuropeptide K.
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