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NEXTflex Small RNA-Seq Kit v3 Library Prep Kit


The NEXTflex™™ Small RNA-Seq Kit v3 uses patented technology to provide a significantly reduced-bias small RNA library prep for Illumina sequencing platforms with both gel-free and low-input options.

 

Bioo Scientific'’s approach to reducing ligation-associated bias uses adapters with randomized bases at the ligation junctions, resulting in greatly decreased bias compared to standard protocols. This reduction in bias results in data that more accurately represents abundances of the small RNAs in the starting material.

 

In addition, this reduction of bias allows more miRNAs to be detected with fewer total reads, increasing efficiency and reducing cost for small RNA sequencing.

 

PAGE purification, required for traditional small RNA library prep, is tedious, time consuming, limits throughput, and prevents start-to-finish automation. The NEXTflex Small RNA-Seq Kit v3 allows for gel-free small RNA library prep.

 

This is possible thanks to the dual approach used for adapter-dimer reduction. Unprecedented reduction of adapter-dimer formation allows completely gel-free small RNA library prep when starting with ≥200 ng of total RNA.

 

Libraries prepared with the NEXTflex Small RNA-Seq kit v3 have a higher proportion of reads mapping to miRNAs

 

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Watch our slideshare presentation below and download a copy of the manual here.

 

 




Small rna seq

Reduced Bias Small RNA Library Prep