Affinity Biosciences - Research Together



Affinity Biosciences - Research Together

 

Affinity Biosciences is a provider of innovative tools for signal transduction research with a focus on well validated phospho-specific antibodies.

 

Use our search to find your antibody (e.g. Ser724, IRE1 or Phospho-IRE1 etc).

 

Well Validated Phospho-specific Antibodies

Validation is important for any antibody, but for Phospho-specific antibodies it is even more important as specificity is truly key to their use.

 

Affinity antibody validation begins with Mass Spec (MS) analysis of the blocking peptide to ensure the peptide sequence is accurate, followed by checking that the antibody and the blocking peptide correspond correctly, with the peptide blocking the antibody in Western Blotting of research samples showing blocking and control lanes without the peptide to show binding of the target.

 

Images are provided on the datasheets of Western blots and use in other applications such as Immunohistochemistry as appropriate.

 

As an example, details of the validation of our Phospho-IRE1 (Ser724) - AF7150 antibody have been provided below:

 

Western Blot, IHC, Immunofluorescence and Peptide-ELISA for Anti Phospho-IRE1 (Ser724).

 

pIRE1 WB  pIRE1 IHC

pIRE1 Immunofluorescence  pIRE1 ELISA

Phospho-IRE1 (Ser724) - AF7150 Datasheet

 

Affinity also produce more than 500 inhibitors and SiRNAs.

 

Additionally all affinity products are covered by our 100% antibody guarantee, so you can try them without worrying whether they will work. We know they work well, but we understand you may be sceptical having tried other antibodies in the past, so now you can try them without worrying.

If they don't work, we'll give you your money back.

 

 

Other Highlighted Antibodies:

 

Anti-Phospho-ERK1/2 Antibody (Thr202/Tyr204) - AF1015

29 Citations

 

Anti-Phospho-Akt Antibody (Ser473) - AF0016

28 Citations

 

Phospho-mTOR Antibody (Ser2448) - AF3308

14 Citations 

 

Phospho-p70 S6 Kinase Antibody (Thr389/412) - AF3228

9 Citations

 

Use our search at the top of the page to find your antibody

(e.g. Ser724, IRE1 or Phospho-IRE1 etc).

 

Questions? Email us at tech@nktscientific.com.