Next Generation Sequencing


NGS Library prep kits and barcodes for DNA-Seq, RNA-Seq, qRNA-Seq, directional RNA-Seq, Small RNA-Seq, Amplicon-Seq and more


Bioo Scientific NEXTflex™ Sequencing Kits & Barcodes have the best ligation efficiency available in a library preparation kit (as shown in these white papers) ensuring you maximise your library diversity and see more of your sample in sequencing.

Share on Facebook  Share on Twitter  Share on LinkedIn

 


 

Nextflex Rapid Kits (DNA-Seq and RNA-Seq) offer streamlined protocols with minimal hands on time, and have validated automation protocols for PerkinElmer's Sciclone NGS Workstation. Protocols are available for NEXTflex DNA-Seq, RNA-Seq and Metagenomics library prep kits.

Nkt nextflex features apr14

 

Follow us on Twitter: @NKTSciNGS

 


 

FEATURED KIT: NEXTflex Small RNA-Seq Kit v3

 

The NEXTflex™™ Small RNA-Seq Kit v3 uses patented technology to provide a reduced-bias small RNA library prep for Illumina sequencing with both gel-free and low-input options. This reduction in bias provides data that better represents true abundances of small RNAs in the starting material and allows more miRNAs to be detected with fewer total reads, increasing efficiency and reducing cost for small RNA sequencing. Click here to read more...

 

Bioo Small RNA Seq

 

Share on Facebook  Share on Twitter  Share on LinkedIn

 



Illumina Compatible Library Prep Sequencing Kits

 

Illumina compatible DNA sequencing kits

NEXTflex™ Rapid DNA-Seq kits and barcodes

NEXTflex™ Rapid DNA-Seq Kit BUNDLE with DNA barcodes

NEXTflex™ ChIP-Seq kits and barcodes

NEXTflex™ PCR-Free DNA-Seq kits and barcodes

NEXTflex™ Cell Free DNA-Seq kit and barcodes

NextPrep-Mag™ cfDNA Isolation Kits

Extract cfDNA from human plasma/serum or urine for cell free DNA-Seq

NEXTflex™ mitochondrial DNA-Seq kit and barcodes

 

Illumina compatible disease panel sequencing kits

Illumina compatible BRCA1 and BRCA2 amplicon panels (for FFPE and non FFPE samples) 

Illumina compatible Mediterranean Fever amplicon panel (for fresh or frozen samples) 

Illumina compatible Diabetes-4 amplicon panel

Illumina compatible HBOC-3 amplicon panel

Illumina compatible CEBPA amplicon panel

Illumina compatible Phenylketonuria amplicon panel

Illumina compatible TP53 amplicon panel

Illumina compatible Nephrotic Syndrome-1 amplicon panel

Illumina compatible Periodic Fever-2 amplicon panel

Illumina compatible TP53 amplicon Panel for FFPE

Illumina compatible Cystic Fibrosis amplicon panel

Illumina compatible MODY-1 amplicon panel

Illumina compatible MODY-2 amplicon Panel

 

Illumina compatible Target Capture sequencing kits

NEXTflex™ Rapid Pre-Capture Combo kit (NimbleGen SeqCap EZ Library Compatible)

NEXTflex™ Pre-Capture Combo kit (NimbleGen SeqCap v3 Compatible)

 

NEXTflex™ Pre- and Post- Capture Combo kit (Agilent SureSelect XT and XT2 compatible)

 

NEXTflex™ DNA barcodes and barcode blockers 

 

Illumina compatible Metagenomics sequencing kits

NEXTflex™ 16S V1-V3 Amplicon-Seq kits and barcodes

NEXTflex™ 16S V4 Amplicon-Seq kits and barcodes

NEXTflex™ 18S ITS Amplicon-Seq kits

 

Sciclone NGS Workstation Automation Guide for NEXTflex Metagenomics Kits

 

Illumina compatible Epigenetics sequencing kits

NEXTflex™ Methyl-Seq kits and barcodes

NEXTflex™ Bisulfite Seq kits and barcodes

 

Illumina compatible RNA, qRNA and Small RNA sequencing kits

NEXTflex™ Rapid RNA-Seq kits and barcodes

NEXTflex™ Rapid Directional RNA-Seq kits and barcodes

 

NEXTflex™ qRNA-Seq kits and barcodes

NEXTflex™ Rapid Directional qRNA-Seq kits and barcodes

 

NEXTflex™ Rapid Directional mRNA-Seq Kit BUNDLE with barcodes and beads

NEXTflex™ Poly(A) beads for batch purification of mRNA from total RNA.

 

NEXTflex™ Small-RNA Seq kits and barcodes with randomised adapters

 


 

Ion Torrent-Based Compatible Sequencing Kits

 

Ion Torrent compatible DNA sequencing kits

NEXTflex™ DNA-Seq Kits and Barcodes for Ion Systems

NEXTflex™ Cell Free DNA-Seq Kits and Barcodes for Ion System

 

Ion Torrent compatible Amplicon Panel sequencing kits

Ion compatible BRCA1 and BRCA2 Amplicon Panels (for FFPE and non FFPE Samples)

 


 

NGS Blog and White Papers

 

For NGS tech tips, news and exciting developments visit the Bioo Scientific NGS Blog:

The "What's Next in NGS?" Blog

 

And read our application notes and white papers:

 

  Share on Facebook  Share on Twitter  Share on LinkedIn

 

DNA:

Tools for Enhancing Sequence Diversity and Reducing Bias in DNA-seq Next Generation Sequencing (NGS) Library Preparation

Achieving High Coverage and Yield from GC and AT Rich Genomes in NGS Library Preparation

Comparison of Yield and Size Distribution of cfDNA Extracted from Human Plasma

 

Small RNA:

Randomized Adapters for Reducing Bias in Small RNA-Seq Libraries 

RNA Extraction and Small RNA Library Production for Next Generation Sequencing (NGS)

 

RNA:

Quantitative, Directional and Standard RNA-Seq

Nature Methods Application Note: "Molecular indexing for improved RNA-Seq analysis in NGS"

Directional qRNA-Seq Combining the Power of Stranded RNA-Seq with the Quantitative Precision of Molecular Labels

 

Metagenomics:

16S rRNA Amplicon Sequencing Offers Enhanced Metagenomic Detection