Immunohistochemistry


Antibodies, stains and detection kits for immunohistochemistry (IHC)


Newmarket Scientific provides reagents used in Immunohistochemistry including:

Antibodies 

Detection kits

Buffers 

PAP pens

 

Please use the search facility above to find the product you are looking for or if you are having difficulty locating it or have any questions, simply email us at tech@nktscientific.com or use our contact form and we'll look for you.

 



Optibodies from Nordic Biosite

 

You have most likely been using antibodies in Immunohistochemistry for some time and maybe you are getting good results, but Optibodies are on another level.

 

In producing the Optibodies range we ensure that every antibody provides beautifully optimised staining. This is the key aim of the Optibodies range and any antibody that does not perform to this standard simply does not make it in to the range.

 

Optibody™ optimisation involves a great number of tests in order to find the optimal staining conditions.

 

Antigen Retrieval

Each antigen has a preferred method of antigen retrieval such as heat induced epitope retrieval using citrate buffer, as well as an enzymatic retrieval process, but the majority of antigens need alkaline antigen retrieval conditions for truly optimal staining. For this reason, Optibodies are all selected according to the performance in alkaline antigen retrieval.

 

Concentration

Antibodies generally have an optimal concentration for use, depending on the affinity of the antibody for its target and expression level of the antigen. Antibodies optimised with tissues expressing high levels of antigen may prove inadequate when staining tissues with low antigen expression. So Optibodies are optimised on a variety of tissue types and antigen expression levels to verify the performance of the Optibody in a range of situations.

 

Internal and External QC

To ensure the high quality of every Optibody, as well as our own high level of internal quality control, we also take part in external quality assurance programs to ensure our antibodies work optimally in the hands of others too.

 

Other key criteria

Another key target for each Optibody is specificity and high affinity towards the target. They are also tailored to be flexible and reliable in use, with high consistency between lots and excellent stability.

 

More information on the full Optibodies range is available on this link.

 



Neuroscience Antibodies

 

The ImmunoStar range of antibodies for neuroscience research are put through extensive testing before release to ensure both high quality and high titer. This provides excellent reliability and lot-to-lot consistency. The antibodies have also been specifically tested for use in immunohistochemistry.

 

This range of primary antibodies was originally provided by DiaSorin (formerly INCSTAR), but in 2001 the range became Immunostar. As these antibodies have been available for many years, there are multiple references citing their use.

 

 

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Details of the full range of 60+ well referenced neuroscience antibodies are available on this link.

 



Ultra Sensitive Immunohistochemistry

Monosan IHC detection kits have been designed for the detection of antigens in fixed paraffin-embedded tissue sections, frozen tissue sections and cytological samples.

 

The kits have been developed for use in combination with monoclonal and polyclonal primary antibodies and sera from mouse, rabbit, rat, and guinea pig

 

Monosan Plus AP Polymer Kitis are highly sensitive detection kits intended for use in immunohistochemistry and immunocytochemistry. The enzyme polymer in this kit consists of several molecules of secondary antibodies covalently bound to several molecules of alkaline phosphatase (AP). 

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Visualisation occurs via an enzyme-substrate reaction in the presence of a colourising reagent which permits microscopical analysis. 

 

These kits provide very clear staining, but have been produced to be low cost, making them the perfect kit for all IHC staining - manual, automated, high sensitivity and every day use.

 

Monosan Plus kits are available to a wide range of primary antibody species including mouse, rabbit, goat, armenian hamster, guinea pig, chicken, sheep, rat specific (no cross reaction with mouse), and mouse specific (no cross reaction with rat) antibodies.

 

More information is available on the links below below or please feel free to use our contact form or email tech@nktscientific.com should you have further questions.