Bioo Scientific

Newmarket Scientific is the exclusive UK and Ireland Distributor for Bioo Scientific.

NGS Library Prep Kits - NEXTflex

Bioo Scientific offers a wide range of reagents for life science with a focus on Next Generation Sequencing Library Prep Kits, this rapidly expanding line of life science kits and reagents includes kits for a wide range of NGS sample types as well as purification kits.

NEXTflex Rapid DNA-Seq Kit 2.0

The NEXTflex™™ Rapid DNA-Seq Kit 2.0 is designed to prepare multiplexed libraries for single or paired-end sequencing using Illumina® platforms. This enhanced NEXTflex® kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time-consuming steps in library preparation.

Libraries prepared with NEXTflex® rapid DNA-Seq kit 2.0 show higher yield than libraries prepared with Competitor K's kit using the same number of PCR cycles from a 250 ng and 1 ng input .

For labs that routinely run DNA sequencing, the NEXTFLEX® rapid DNA-seq kit 2.0 that boasts high yields of quality sequencing libraries from fragmented genomic DNA in the form of a convenient, all-inclusive kit that includes the library prep reagents, barcoded adapters, and cleanup beads. Labs have the option of multiplexing up to 384 samples using the NEXTFLEX-HT™ barcodes or up to 192 samples using the NEXTFLEX® unique dual index barcodes.

 KEY FEATURES

 • Robust yields

 • Quality sequencing data

   » Genome coverage

   » GC bias

   » Mapping rate

   » Mitigation of duplication rate

   » PCR-free compatible workflow

 • Low input, down to 1 ng, compatible workflow

 • Automated on the PE Sciclone® G3 NGS workstation

For more information and pricing follow the link above (NEXTflex™ Rapid DNA-Seq Kit 2.0)

The NEXTflex™™ Small RNA-Seq Kit v3 uses patented technology to provide a significantly reduced-bias small RNA library prep for Illumina sequencing platforms with both gel-free and low-input options.

Bioo Scientific'’s approach to reducing ligation-associated bias uses adapters with randomized bases at the ligation junctions, resulting in greatly decreased bias compared to standard protocols. This reduction in bias results in data that more accurately represents abundances of the small RNAs in the starting material.

In addition, this reduction of bias allows more miRNAs to be detected with fewer total reads, increasing efficiency and reducing cost for small RNA sequencing.

PAGE purification, required for traditional small RNA library prep, is tedious, time consuming, limits throughput, and prevents start-to-finish automation. The NEXTflex Small RNA-Seq Kit v3 allows for gel-free small RNA library prep.

This is possible thanks to the dual approach used for adapter-dimer reduction. Unprecedented reduction of adapter-dimer formation allows completely gel-free small RNA library prep when starting with ≥200 ng of total RNA.

Libraries prepared with the NEXTflex Small RNA-Seq kit v3 have a higher proportion of reads mapping to miRNAs

Watch our slideshare presentation below and download a copy of the manual here.