| Product Detail: |
Mouse anti-JAM-1, clone BV16 (Monoclonal) |
| Antibody Type: |
Monoclonal |
| Host Animal: |
Mouse |
| Species Reactivity: |
human |
| Immunogen: |
Unknown or proprietery to MONOSAN and/or its suppliers |
| Applications: |
IHC-fr,FC,IF,IP,WB |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
The monoclonal antibody BV16 recognizes the human junction adhesion molecule (JAM)-A. Together with JAM-C (JAM-2) and JAM-B (VE-JAM or JAM-3), JAM-A belongs to a family of adhesion proteins with a V-C2 immunoglobulin domain organization and their molecular weight is about 30-40 kDa. JAMs are important for a variety of cellular processes, including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. JAM-A is expressed by endothelial and epithelial cells, platelets, neutrophils, monocytes, lymphocytes and erythrocytes. Like all other JAMs, JAM-A plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-A also plays an important role in leukocyte transmigration. Leukocyte transmigration can be blocked by antibodies and by soluble JAM-A/Fc fusion proteins. Homophilic JAM-A interactions between leukocytes and the endothelium but also heterophilic interactions of JAM-A with the beta2-integrin leukocyte function-associated antigen-1 (LFA-1) are considered to actively guide leukocytes during transmigration. Several studies imply a role for JAM-A in the initiation of atherosclerosis since JAM-A is upregulated on early atherosclerotic endothelium. The adhesion of activated platelets on the activated endothelium is mediated by homophilic interactions of JAM-A. |
| Antibody Isotype: |
IgG1 |
| Monosan Range: |
MONOSAN |
| Clone: |
BV16 |
| Concentration: |
100 ug/ ml |
| Storage buffer: |
PBS with 0.1% BSA and 0.02% sodium azide |
| Storage: |
2-8°C |
| References 1: |
Bazzoni; G et al. J Biol Chem 2000; 275: 20520 |
| References 2: |
Luo, Y et al Invest Ophthalmol Vis Sci 2006, 47: 3644 |
| References 3: |
Faure; V et al. Int Immunol 2006; 18: 1453 |
| References 4: |
Vetrano S et al. Gastroenterol 2008; 135: 173 |
|
| Product Detail: |
Mouse anti-CD19, clone MX016 (Monoclonal) |
| Antibody Type: |
Monoclonal |
| Host Animal: |
Mouse |
| Species Reactivity: |
Human |
| Immunogen: |
Unknown or proprietery to MONOSAN and/or its suppliers |
| Applications: |
IHC-P (1:200-1:400) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
CD 19 is expressed only on B-cells and follicular dendritic cells. It is a specific and sensitive marker of B-cells widely expressed from early pre-B stages, normal B-cells and normal plasma cells (staining is weaker than normal B-cells and a subpopulation may lack expression). It is considered a positive regulator of both intrinsic and stimulus-dependent pathways in B-lymphocytes. CD19 is useful in identification of B-cell lineage of majority of B-cell neoplasms but appears to be less useful in subclassifying of B-cell neoplasms in histological material. It appears to be potentially useful additional marker of follicular dendritic cell tumours. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Tonsil or lymph node. Staining membranous |
| Antibody Isotype: |
IgG |
| Monosan Range: |
MONOSAN |
| Clone: |
MX016 |
| Concentration: |
n/a |
| Format: |
Concentrate |
| Storage buffer: |
Tissue culture supernatant containing 15mM sodium azide |
| Storage: |
2-8°C |
| References 1: |
Kochenderfer J N, Dudley M E, Feldman S A, et al, Blood, 2012, 119(12): 2709-2720. |
| References 2: |
Masir N, Marafioti T, Jones M, et al, Histopathology, 2006, 48(3): 239-246. |
|
| Product Detail: |
Substance P Antibody |
| Background Info: |
Raised to Substance P coupled to KLH with carbodiimide . |
| Product Type: |
Antibody - Antibodies |
| Antibody Type: |
polyclonal |
| Format: |
Lyophilised |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Bird, Bullfrog, Cat, Chick, Chicken, Dog, Dolphin, Doves, Equine, Fish, Frog, Gerbil, Goat, Goldfish, Guinea Pig, Hamster, Horse, Human, Monkey, Mouse, Mudpuppy, Narwhal, Parrot, Pig, Pig, Pigeon, Quail , Rabbit, Ram (Sheep), Rat, Ratfish, Salmon, Scorpaena Porcus (Fish), Sea Lamprey, Sheep, Snail, Sparus Aurata (Fish), Squalus Acanthias (Dogfish), Squirrel, Starfish, Sting Ray, Sturgeon (Fish), Three-Spined Stickleback (Fish), Trout, Turtle, Vole, Zebra Finch (Bird), Zebrafish |
| Immunogen: |
Substance P |
| Applications: |
Immunohistochemistry, Immunocytochemistry, immunofluorescence |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
The Substance P antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat substantia nigra and spinal cord using biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500-1/1000 in PBS/0.3% Triton X-100 - Cy3 Fluorchrome and 1/6000-1/8000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. The specificity of the antiserum for Substance P was demonstrated using soluble pre-adsorption with the peptides in question at a final concentration of 10 µg of peptide per mL of diluted antiserum. Substance P immunolabeling was completely abolished by pre-adsorption with Substance P. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: neurokinin A, neurokinin B, somatostatin and neuropeptide K. |
| Gene Symbol: |
Tacr1 |
| NCBI Gene Aliases: |
Tac1 |
| PubMed IDs: |
16680762, 16131542, 14694537, 11145532, 10571116, 6187783, 2482114, 2445435, 1553558, 25932952 |
| Product category: |
Cancer, Dementia, Dementia (aging), Diabetes, Huntington's, Inflammatory Bowel Disease, Pain, Parathyroid, Parkinson's, Schizophrenia, Stem Cell, Transcription Factor, Tumor Markers, Viral, |
|
| Background Info: |
Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized |
| Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana, Nicotiana tabcum, Salicornia sp., Solanum lycopersicum, Zea mays |
| Expected Species: |
Marchantia polymorpha, Pisum sativum, Riciuns communis, Oryza sativa, Triticum aestivumSpecies of your interest not listed? Contact us |
| Immunogen: |
KLH-conjugated synthetic peptide derived from C-terminal part of Zea mays gamma-ECS Q8W4W3 |
| Applications: |
Immunolocalization (IL), Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
Antibodies has been used in immunolocalization on Arabidopsis thaliana. |
| Application Details: |
1 : 5000 (IL), 1 : 5000 (WB) |
| Purity: |
Total IgG. Protein G purified in PBS pH 7.4. |
| Reconstitution: |
For reconstitution add 100 µl of sterile water |
| Molecular Weight: |
50 kDa |
| Not reactive in: |
No confirmed exceptions from predicted reactivity are currently known |
| Selected references: |
Shull et al. (2019). Anatase TiO2 nanoparticles induce autophagy and chloroplast degradation in thale cress (Arabidopsis thaliana). Environ Sci Technol. 2019 Jul 29. doi: 10.1021/acs.est.9b01648.Bal žov et al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013.Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.Ghanta et al. (2011). Nicotiana tabacum overexpressing γ-ECS exhibits biotic stress tolerance likely through NPR1-dependent salicylic acid-mediated pathway. Planta 233(5):895-910. |
| Special application note: |
Total IgG concentration is 5,2 g/ l |
| UniProt number: |
Q8W4W3 |
| TAIR number: |
AT4G23100 |
| Research area: |
Arabidopsis thaliana antibodies |
| Code: |
Stress20 |
|
