The NSR Prestained Protein Ladder is a three colour protein standard with 13 prestained proteins covering molecular weights from 3.5 to 245 kilodaltons. It has been designed for use in most general applications for a protein ladder with a broad range of protein sizes, key reference bands and excellent Western transfer efficiency (tested with PVDF, nylon and nitrocellulose membranes).
Background Info:
Most of the proteins are covalently coupled with a blue chromophore apart from two reference bands, one stained red at 75 kDa and one stained green at 25 kDa for easy reading of the ladder and to aid in the monitoring of protein separation.
The NSR Prestained Protein Ladder is supplied ready to use in gel loading buffer.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE.
Arrange for our Protein ladder to be in your stores and receive a free vial. Email us for more details - tech@nktscientific.com.
Product Type:
NS Reagents Protein Ladder
Format:
Buffer: 20 mM Tris-phosphate, pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, 15 % (v/v) Glycerol.
Storage Temp:
Store at 25°C, for 2 weeks or 4°C for 3 months. For long-term storage, store at -20°C.
The shelf life is 1 year from receipt when stored frozen, however the NSR Prestained Protein Ladder should be stable for many years if stored frozen and excessive freeze thaw cycles are avoided.
The fragments in our 50bp ladder range from 50-1,500 base pairs, with the 200, 500 and 1,200 base pair bands having increased intensity to act as key reference points. There are 17 bands in total.
The expected mass of DNA in each band (based on a total of 0.56 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE. .
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 50bp ladder is provided ready to use with orange G as the tracking dye.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The fragments in our 50bp ladder range from 50-1,500 base pairs, with the 200, 500 and 1,200 base pair bands having increased intensity to act as key reference points. There are 17 bands in total.
The expected mass of DNA in each band (based on a total of 0.56 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE. .
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 50bp ladder is provided ready to use with orange G as the tracking dye.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The fragments in our 1Kbp ladder range from 250-10,000 base pairs, with the 1K and 3K base pair bands having increased intensity to act as key reference points. There are 13 bands in total.
The expected mass of DNA in each band (based on a total of 0.5 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE. .
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 1Kbp ladder is provided ready to use with bromophenol blue & xylene cyanol FF included as tracking dyes.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The fragments in our 1Kbp ladder range from 250-10,000 base pairs, with the 1K and 3K base pair bands having increased intensity to act as key reference points. There are 13 bands in total.
The expected mass of DNA in each band (based on a total of 0.5 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE.
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 1Kbp ladder is provided ready to use with bromophenol blue & xylene cyanol FF included as tracking dyes.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The fragments in our 100bp ladder range from 100-1,500 base pairs, with the 500 and 1,500 base pair bands having increased intensity to act as key reference points. There are 11 bands in total.
The expected mass of DNA in each band (based on a total of 0.5 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE. .
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 100bp ladder is provided ready to use with orange G & xylene cyanol FF included as tracking dyes.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The fragments in our 100bp ladder range from 100-1,500 base pairs, with the 500 and 1,500 base pair bands having increased intensity to act as key reference points. There are 11 bands in total.
The expected mass of DNA in each band (based on a total of 0.5 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE.
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 100bp ladder is provided ready to use with orange G & xylene cyanol FF included as tracking dyes.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The Blook gel illumination system for the visualisation of DNA gels stained with ethidium bromide or alternatives such as NovelJuice (cat no LD001). This CE marked Blue Light LED system is more reliable than UV lamp transilluminators as the LEDs have a life time of 30,000 hours of continual use. The unit also includes an Amber filter to allow visualisation of the gel whilst protecting the eyes. The Blook has no external powerpack and takes up a minimal amount of bench space. Plus it is so light it can be stored on a standard lab shelf easily when not in use.
[SPECIFICATIONS] Dimensions (mm): 210D X 210W X 30H >> Viewing surface (mm): 120 X 70 >> Wavelength (nm): 470 >> Amber filter: amber filter shield with metal frame >> LED arrangements: matrix for two-side illumination >> LED lifetime:50,000 hours >> Power: No external power supply required >> Compatible with mini gel size (mm): 110 X 60 & 55 X 60 >> Weight (kg): 2.3 >>
Novel Juice is a non-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon Blue Light or UV illumination of agarose gels. Supplied in GeneDireXs 6X DNA Loading Buffer, Novel Juice can be used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. We believe Novel Juice is the most sensitive stain available for detecting double-stranded DNA (dsDNA). It also contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for visually tracking the DNA migration on your gel without illumination. It is a safe , non-hazardous alternative to Ethidum Bromide, having been independently tested.
Background Info:
[APPLICATION] (1) Vortex Novel Juice for 10 seconds prior to use. (2) Dilute 1 part Novel Juice with 5 parts DNA sample or ladder and mix. (3) Load sample and run according to your standard lab procedures. (4) After electrophoresis, remove the gel and place it on UV or a visible-light transilluminator to visualize the bands. (5) Gels can also be post-stained with Ethidium Bromide if desired.
Gel Stain, Ethidium Bromide Alternative, DNA stain, nucleic acid dye, nucleic acid stain, DNA dye
Additional Info:
Contains Tracking Dyes: Bromophenol Blue, Xylene Cyanol FF, and Orange G. Store at room temperature or at 4°C up to 12 months. For longer periods, store at -20°C. Novel Juice Dye is light sensitive and should be stored protected from light.
Novel Juice is a non-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon Blue Light or UV illumination of agarose gels. Supplied in GeneDireXs 6X DNA Loading Buffer, Novel Juice can be used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. We believe Novel Juice is the most sensitive stain available for detecting double-stranded DNA (dsDNA). It also contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for visually tracking the DNA migration on your gel without illumination. It is a safe , non-hazardous alternative to Ethidum Bromide, having been independently tested.
Background Info:
[APPLICATION] (1) Vortex Novel Juice for 10 seconds prior to use. (2) Dilute 1 part Novel Juice with 5 parts DNA sample or ladder and mix. (3) Load sample and run according to your standard lab procedures. (4) After electrophoresis, remove the gel and place it on UV or a visible-light transilluminator to visualize the bands. (5) Gels can also be post-stained with Ethidium Bromide if desired.
Gel Stain, Ethidium Bromide Alternative, DNA stain, nucleic acid dye, nucleic acid stain, DNA dye
Additional Info:
Contains Tracking Dyes: Bromophenol Blue, Xylene Cyanol FF, and Orange G. Store at room temperature or at 4°C up to 12 months. For longer periods, store at -20°C. Novel Juice Dye is light sensitive and should be stored protected from light.
Novel Juice is a non-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon Blue Light or UV illumination of agarose gels. Supplied in GeneDireXs 6X DNA Loading Buffer, Novel Juice can be used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. We believe Novel Juice is the most sensitive stain available for detecting double-stranded DNA (dsDNA). It also contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for visually tracking the DNA migration on your gel without illumination. It is a safe , non-hazardous alternative to Ethidum Bromide, having been independently tested.
Background Info:
[APPLICATION] (1) Vortex Novel Juice for 10 seconds prior to use. (2) Dilute 1 part Novel Juice with 5 parts DNA sample or ladder and mix. (3) Load sample and run according to your standard lab procedures. (4) After electrophoresis, remove the gel and place it on UV or a visible-light transilluminator to visualize the bands. (5) Gels can also be post-stained with Ethidium Bromide if desired.
Gel Stain, Ethidium Bromide Alternative, DNA stain, nucleic acid dye, nucleic acid stain, DNA dye
Additional Info:
Contains Tracking Dyes: Bromophenol Blue, Xylene Cyanol FF, and Orange G. Store at room temperature or at 4°C up to 12 months. For longer periods, store at -20°C. Novel Juice Dye is light sensitive and should be stored protected from light.
Trx (Thioredoxin 1) is a redox protein with a primary domain conserved across a number of Trx family members. The protein contains a conserved catalytic site Cys-Gly-Pro-Cys. The protein is used as a fusion tag in a number of molecular biology applications.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Rad22 protein of Schizosaccharomyces pombe (469 aa, 52 kDa) is a functional and structural homologue of Saccharomyces cerevisiae and human Rad52 proteins, which play a major role together with Rhp51 in genetic recombination and recombination repair, by mediating strand annealing reaction between homologous DNA strands.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Saccharomyces pombe
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Purified, full length, recombinant Rad22 protein from Saccharomyces cerevisiae, UniProt: P36592 overexpressed in E. coli
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunoprecipiatation (IP), Western blot (WB)
Lehmann (1996). Molecular biology of DNA repair in the fission yeast Schizosaccharomyces pombe. Mutat Res. 1996 Aug 8;363(3):147-61. doi: 10.1016/0921-8777(96)00017-1. PMID: 8765156.
Beta-galactosidase is an exoglycosidase which hydrolyzes the β-glycosidic bond formed between agalactose and its organic moiety. It may also cleave fucosides and arabinosides but with much lower efficiency. It is an essential enzyme in the human body. Deficiencies in the protein can result ingalactosialidosis or Morquio B syndrome. In E. coli , the gene of β-galactosidase, the lacZ gene, is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low. It is commonly used in molecular biology as a reporter marker to monitor gene expression. It also exhibits a phenomenon called α-complementation which forms the basis for the blue/white screening of recombinant clones. This enzyme can be split in two peptides, LacZα and LacZΩ, neither of which is active by itself but when both are present together, spontaneously reassemble into a functional enzyme. This property is exploited in many cloning vectors where the presence of the lacZα gene in a plasmid can complement in trans another mutant gene encoding the LacZΩ in specific laboratory strains of E. coli . However, when DNA fragments are inserted in the vector, the production of LacZα is disrupted, the cells therefore show no β-galactosidase activity. The presence or absence of an active β-galactosidase may be detected by X-gal, which produces a characteristic blue dye when cleaved by β-galactosidase, thereby providing an easy means of distinguishing the presence or absence of cloned product in a plasmid. E. coli β-Galactosidase consists of 1,024 amino acids with molecular mass of 116 kDa and functional form is a homotetramer.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. , Do not store this antibody below -20 °C
Host Animal:
Rabbit
Species Reactivity:
Beta galactosidase (E.coli) and beta galactosidase tagged proteins
Immunogen:
Full length Beta galactosidase from E.coli UniProt: B7UJI9
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
OEP 75 or Toc75; Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded -barrel (EMBO J. (1995) 14:11, 2436-2446); In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87; Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane; YaeT; outer membrane protein assembly complex, (TIGR03303); FhaC; Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum, Physcomitrium patens, Pisum sativum, Spinacia oleracea, Thellungiella salsuginea, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus sp.
Expected Species:
Aegilops tauschii, Ananas comosus, Anthurium amnicola, Capsicum annuum, Catalpa bungei, Cicer arietinum, Cucumis melo, Glycine soja, Gossypium arboreum, Medicago truncatula, Morus notabilis, Nelumbo nucifera, Nicotiana sylvestris, Noccaea caerulescens, Populus trichocarpa, Ricinus communis, Sorghum bicolor, Theobroma cacao, Zostera marina, Vigna radiata, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
Pisum sativum TOC75; Predicted POTRA Domain #3; Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. UniProt: Q43715
Applications:
Flow Cytometry (Flow cyt), Immunolocalization (IL), Western blot (WB)
88 | 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Sasaki and Yamamoto (2015). Arabidopsis LAZY1 is a peripheral membrane protein of which the carboxy-terminal fragment potentially interacts with microtubules. Copyright 2015 The Japanese Society for Plant Cell and Molecular Biology, Plant Biotechnology, 32, 1–6 (2015) DOI: 10.5511/plantbiotechnology.15.0116aVera-Estrella et al. (2014). Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics. 2014 Jun 2. pii: S1874-3919(14)00288-7. doi: 10.1016/j.jprot.2014.05.018.Hsueh et al. (2014). The chloroplast outer envelope protein P39 in Arabidopsis thaliana belongs to the Omp85 protein family. Proteins. 2014 Nov 17. doi: 10.1002/prot.24725.
Apolipoprotein A5 (ApoA5) is fast gaining attention as a key regulator of serum triglyceride concentrations. An ApoA5 mouse knock-out model produced an approximately four fold increase in serum triglycerides, whereas a knock-in model with human ApoA5 produced 5070% lower concentrations of mouse serum triglycerides. In addition, peroxisome proliferator-activated receptor- agonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. Despite these compelling molecular biology data, relatively little is known about ApoA5 protein in human serum. This antibody pair detected recombinant apoa5 protein in sandwich ELISA format and could be potential reagents for the development of clinical diagnostic kits.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Host Animal:
mouse
Immunogen:
Purified recombinant fragment of human Apoa5 expressed in E. Coli.
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